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001-es BibID:BIBFORM058347
Első szerző:Guttmann Miklós
Cím:Comparative glycoprofiling of HIV gp120 immunogens by capillary electrophoresis and MALDI mass spectrometry / Miklós Guttman, Csaba Váradi, Kelly K. Lee, András Guttman
Dátum:2015
ISSN:0173-0835
Megjegyzések:The Human Immunodeficiency Virus (HIV) envelope glycoprotein (Env) is the primary antigenic feature on the surface of the virus and is of key importance in HIV vaccinology. Vaccine trials with the gp120 subunit of Env are ongoing with the recent RV144 trial showing moderate efficacy. gp120 is densely covered with N-linked glycans that are thought to help evade the host's humoral immune response. To assess how the global glycosylation patterns vary between gp120 constructs, the glycan profiles of several gp120s were examined by capillary electrophoresis with laser induced fluorescence detection and MALDI-MS. The glycosylation profiles were found to be similar for chronic vs. transmitter/founder isolates and only varied moderately between gp120s from different clades. This study revealed that the addition of specific tags, such as the gD tag used in the RV144 trial, had significant effects on the overall glycosylation patterns. Such effects are likely to influence the immunogenicity of various Env immunogens and should be considered for future vaccine strategies, emphasizing the importance of the glycosylation analysis approach described in this paper.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
HIV glycosylation
Megjelenés:Electrophoresis 36 : 11-12 (2015), p. 1305-1313. -
További szerzők:Váradi Csaba (1988-) (molekuláris biológus) Lee, Kelly K. Guttman András (1954-) (vegyészmérnök)
Pályázati támogatás:NIH F32-GM097805
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Internet cím:DOI
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