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1.

001-es BibID:BIBFORM075927
Első szerző:Jakab Attila (szülész-nőgyógyász, endokrinológus)
Cím:Sperm biomarkers in male infertility and testicular cancer : genetic, epigenetic and functional examinations / Jakab Attila, Benyó Mátyás, Bazsáné Kassai Zsuzsa, Békési Gyöngyi, Mokánszki Attila, Oláh Éva, Molnár Zsuzsanna
Dátum:2016
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Giornale Italiano di ostetricia e ginecologia. - 38 : 1 (2016), p. 103-106. -
További szerzők:Benyó Mátyás (1982-) (urológus) Bazsáné Kassai Zsuzsa (1946-) (embriológus) Békési Gyöngyi Mokánszki Attila (1983-) (molekuláris biológus Ph.D hallgató) Oláh Éva (1943-2019) (gyermekgyógyász, klinikai genetikus) Molnár Zsuzsanna (1980-) (szülész-nőgyógyász, labor szakorvosjelölt, urológus)
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2.

001-es BibID:BIBFORM061576
Első szerző:Jakab Attila (szülész-nőgyógyász, endokrinológus)
Cím:Correlation between sperm chromatin decondensation, density, aneuploidy and hyaluronic binding capacity / Jakab, A., Benyó, M., Mokanszki, A., Bazsáné, K. Z., Molnár, Z.
Dátum:2015
ISSN:0268-1161
Megjegyzések:Study question: Whether there is relation between sperm chromatin deconden- sation, concentration, hialuronic acid (HA) binding capacity and aneuploidy of semen samples of fertile, normozoospermic and infertile, oligozoospermic men?Summary answer: There are correlations between sperm condensation and the examined biomarkers, irrespective to fertility status. The sperm conden- sation rate, HA bindig capacity related fertility potential and sperm density are strongly related. Also, there is relation between sperm condensation and aneuploidy rate.What is known already: Importance of sperm biomarkers are increasing in male infertility. Beside genetic integrity and fertilization potential tests, epigen- etic studies came to the front. Chromatin decondensation is related to persistent histones detected by aniline blue, while HA binding capacity reflects genetic integrity and maturity related fertilization potential. Paralel evaluation of test results improves the understanding the regulation mechanisms of spermatogen- esis and fertilization, may help in choosing the most effective therapeutic ap- proach and sperm selection for assisted reproduction.Study design, size, duration: This is a cross-sectional study examining semen of 20 infertile, oligozoospermic and 20 fertile, normozoospermic men attend- ing at the Andrology Center and the Sperm Cryopreservation Laboratory. Participants/materials, setting, methods: The ejaculates were assessed as per WHO guidelines. Hyaluronic acid binding capacity test was the func- tional test. To determine the numerical chromosome aberrations we used multicolor fluorescent in situ hybridization (FISH) for chromosomes X, Y and 17. Aniline blue staining was performed as a nuclear decondensation marker test.Main results and the role of chance: In normozoospermic samples mean sperm concentration was 73,4 M/mL, rate of decondensed sperm nuclei detected by aniline blue 16.2%, HA-binding capacity 82% and estimated an- euploidy rate 4.12%. In the oligozoospermic samples mean sperm concentra- tion was 7.13 M/mL, rate of dencondensed nuclei 41.8%, HA binding capacity 47.5% and the estimated aneuploidy rate 6.17%. All examined parameters dif- fer significantly, if samples below and over 20% decondensation compared. There are inverse correlations between sperm decondensation and sperm con- centration (r = -0.876), as well as sperm decondensation and hyaluronic binding capacity (r = -0.876). Sperm decondensation rate is also related to aneuploidy rate (r = 0.559).Limitations, reason for caution: Data on confounders influencing sperm characteristics such as smoking, occupational or environmental hazards, co- morbidities were not collected.Wider implications of the findings: Although chromatin condensation occurs in late phase of spermatogenesis, it shows correlation with cytogenetic abnor- malities and membrane remodelling, irrespective to the fertility status. It can be explained with a common regulation mechanism, thus malfunction of it can result in various sperm defects on different levels. However, since examined biomarkers reflect different sperm defects, they cannot replace each other. Study funding/competing interest(s): Funding by national/international organization(s) ? This research was supported by the European Union and the State of Hungary, co-financed by the European Social Fund in the framework of TÁMOP 4.2.4.A/ 2-11/1-2012-0001Trial registration number: NA.Keywords: sperm function, sperm biomarkers, sperm decondensation, sperm aneuploidy, hyaluronic binding capacity
Tárgyszavak:Orvostudományok Klinikai orvostudományok idézhető absztrakt
sperm function
sperm biomarkers
sperm decondensation
sperm aneuploidy
sperm hyaluronic bindig capacity
Megjelenés:Human Reproduction. - 30 : Suppl (2015). p. i159. -
További szerzők:Benyó Mátyás (1982-) (urológus) Mokánszki Attila (1983-) (molekuláris biológus Ph.D hallgató) Bazsáné Kassai Zsuzsa (1946-) (embriológus) Molnár Zsuzsanna (1980-) (szülész-nőgyógyász, labor szakorvosjelölt, urológus)
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3.

001-es BibID:BIBFORM055584
035-os BibID:Article ID: P-008
Első szerző:Jakab Attila (szülész-nőgyógyász, endokrinológus)
Cím:Sperm fertilization potential and chromosomal anomalies at decreased sperm concentration : differences between testicular cancer patients and infertile men / A. Jakab, A. Mokánszki, A. Varga, M. Benyó, Z. Kassai, E. Oláh, Z. Molnár
Dátum:2013
ISSN:0268-1161
Megjegyzések:Study questionTo compare the sperm hyaluronic acid (HA) binding capacity and aneuploidy frequency of testicular germ cell carcinoma (TGCC) patients and infertile men without testicular cancer, but with decreased sperm concentrations.Summary answerWhile decreased fertilization potential reflected by low HA binding are related to low sperm count in both groups, elevated frequencies of chromosomal abnormalities are detected only in infertile, but not in TGCC patients. Qualitative spermiogenesis seems to be dissimilarly defected in TGCC and in infertile men without testicular cancer.What is known alreadyQuantitative spermatogenesis is known to be defected in men with TGCC, but little is known about the sperm quality and whether oligozoospermia arises from testicular alteration due the surgery or the tumor. Cryopreservation is the standard choice to preserve fertility. Infertile men with low sperm concentrations have higher frequency of aneuploid spermatozoa, which can be transmitted with assisted reproduction. HA binding capacity related to sperm maturity, viability, normal chromatin structure and fertilization potential.Study design, size, durationThis analysis is part of a prospective cohort study recruiting TGCC patients referred for cryopreservation and infertile men referred for genetic and functional sperm analysis in an academic tertiary center from 2010 to 2012. In this analysis patients with sperm count <40M/mL were included before treatment.Participants/materials, setting, methodsTwenty-one TGCC patients and 26 infertile men were examined. Spermiogram, Hyaluronan Binding Assay (HBA) was performed on native samples, HBA scores (%Bound = 100xBound Motile/Total Motile) were calculated. Sperm aneuploidy and diploidy frequency were examined with triple-color FISH scoring ca.5,000 spermatozoa of each individuals, then estimated numerical chromosome aberrations were calculated.Main results and the role of chanceMean sperm concentration was slightly higher (18.7 M/mL, range 6-35) in TGCC patients (mean age 28.7, range 18-37) than in infertile men (12.3 M/mL, range 1-39, mean age 34.4, 27-42) (p < 0.05). HBA score was 54.1% (range 8-86.5) in TGCC patients and even lower, 38.7% (0-83) in the infertile group (p < 0.05). The X/Y ratio were close to 1:1 in both groups. Estimated numerical chromosome aberrations were higher in the infertile than in the TGCC group (6.08% vs. 4.12%, p < 0.001), with elevations of both autosomal aneuploidies (0.11% vs. 0.07% of disomies 17, p < 0.001) and diploidies (0.30% vs. 0.15%, p < 0.001), but not sex chromosome disomies (0.41% vs. 0.40%, p = 0.1628). Samples normality was analysed using Shapiro-Wilk test, homogenity with Barlett test and the significance level with Two-sample t-probe.Limitations, reason for cautionMean sperm concentration is higher in TGCC patients than in the infertile group, since considering the limited amount for cryopreservation only samples < 6 M/mL were used for study purposes in those men.Wider implications of the findingsLow fertilization potential reflected in decreased sperm count and low hyaluronic binding capacity occurs in both TGCC and infertile patients. However, while the sperm aneuploidy in increased in infertile men, TGCC patients show similar rate to normal population. The presence of cancer does not increase the frequency of sperm numerical chromosomal aberrations. Thus, unlike in oligozoospermic infertile men, semen cryopreservation and assisted reproduction in TGCC do not increase risk of transmitting major genetic defects.Study funding/competing interest(s)Study was founded by the participating institutions and by the PhD scholarships. No competing interest declared.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idézhető absztrakt
sperm
testicular cancer
infertility
aneuploidy
hyaluronic binding capacity
Megjelenés:Human Reproduction. - 28 : S1 (2013), [2] p. -
További szerzők:Mokánszki Attila (1983-) (molekuláris biológus Ph.D hallgató) Varga Attila (1949-) (urológus, andrológus) Benyó Mátyás (1982-) (urológus) Bazsáné Kassai Zsuzsa (1946-) (embriológus) Oláh Éva (1943-2019) (gyermekgyógyász, klinikai genetikus) Molnár Zsuzsanna (1980-) (szülész-nőgyógyász, labor szakorvosjelölt, urológus)
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4.

001-es BibID:BIBFORM039858
Első szerző:Mokánszki Attila (molekuláris biológus Ph.D hallgató)
Cím:Meiotic segregation study of a novel t(3;6)(q21;q23) in an infertile man using fluorescence in situ hybridization (FISH) / Attila Mokánszki, Anikó Ujfalusi, Erzsébet Balogh, Andrea Sümegi, Péter Antal-Szalmás, Zsuzsa Kassai Bazsáné, Zsuzsanna Molnár, Attila Varga, Tamás Sápy, Attila Jakab, Éva Oláh
Dátum:2012
ISSN:1939-6368
Megjegyzések:Male carriers with balanced reciprocal translocations can produce a variable proportion of unbalanced gametes resulting in reproductive failures. The presence of a structural rearrangement may induce an interchromosomal effect. This is characterized by abnormal bivalents not involved in the reorganization thereby yielding non-disjunction, which would present as aneuploid spermatozoa for these chromosomes. In the present case report segregation analysis of the sperm and investigationof interchromosomal effect were carried out using cytogenetic and fluorescence in situ hybridization (FISH) analysis on blood lymphocytes. The karyotype of the patient was 46,XY,t(3;6)(q21;q23). During sperm segregation analysis a total of 2,002 sperms were evaluated, of which 46.8% showed normal/balanced (alternate segregation mode) and 53.2% of sperm showed an abnormal signal pattern. A significant difference in the frequency of the estimated number of chromosome anomalies was observed in the translocation carrier when compared to the normozoospermic group (P < 0.0001) and the oligozoospermicgroup (P < 0.0001). Meiotic segregation analysisof sperm together with aneuploidy assessment for X, Y, and 17chromosomes using FISH allows for the determination of areproductive prognosis in male balanced translocation carriersand can be used for appropriate genetic counseling.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
balanced reciprocal translocation
interchromosomal effect
meiotic segregation analysis
sperm FISH
egyetemen (Magyarországon) készült közlemény
Megjelenés:Systems Biology in Reproductive Medicine. - 58 : 3 (2012), p. 160-164. -
További szerzők:Ujfalusi Anikó (1968-) (gyermekorvos, laboratóriumi szakorvos) Balogh Erzsébet (1949-) (biológus, citogenetikus) Sümegi Andrea (1969-) (biológus) Antal-Szalmás Péter (1968-) (laboratóriumi szakorvos) Bazsáné Kassai Zsuzsa (1946-) (embriológus) Molnár Zsuzsanna (1980-) (szülész-nőgyógyász, labor szakorvosjelölt, urológus) Varga Attila (1949-) (urológus, andrológus) Sápy Tamás (1970-) (szülész-nőgyógyász) Jakab Attila (1964-) (szülész-nőgyógyász, endokrinológus) Oláh Éva (1943-2019) (gyermekgyógyász, klinikai genetikus)
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5.

001-es BibID:BIBFORM039857
Első szerző:Mokánszki Attila (molekuláris biológus Ph.D hallgató)
Cím:Correlation study between sperm concentration, hyaluronic acid-binding capacity and sperm aneuploidy in Hungarian patients / Attila Mokánszki, Zsuzsanna Molnár, Anikó Ujfalusi, Erzsébet Balogh, Zsuzsa Kassai Bazsáné, Attila Varga, Attila Jakab, Éva Oláh
Dátum:2012
ISSN:1472-6483
Megjegyzések:Infertile men with low sperm concentration and/or less motile spermatozoa have an increased risk of producing aneuploid spermatozoa. Selecting spermatozoa by hyaluronic acid (HA) binding may reduce genetic risks such as chromosomal rearrangements and numerical aberrations. Fluorescence in-situ hybridization (FISH) has been used to evaluate the presence of aneuploidies. This study examined spermatozoa of 10 oligozoospermic, 9 asthenozoospermic, 9 oligoasthenozoospermic and 17 normozoospermic men by HA binding and FISH. Mean percentage of HA-bound spermatozoa in the normozoospermic group was 81%, which was significantly higher than in the oligozoospermic (P<0.001), asthenozoospermic (P<0.001) and oligoasthenozoospermic (P<0.001) groups. Disomy of sex chromosomes (P=0.014) and chromosome 17 (P=0.0019), diploidy (P=0.03) and estimated numerical chromosome aberrations (P=0.004) were significantly higher in the oligoasthenozoospermic group compared with the other groups. There were statistically significant relationships (P<0.001) between sperm concentration and HA binding (r=0.658), between sperm concentration and estimated numerical chromosome aberrations (r=-0.668) and between HA binding and estimated numerical chromosome aberrations (r=-0.682). HA binding and aneuploidy studies of spermatozoa in individual cases allow prediction of reproductive prognosis and provision of appropriate genetic counselling. Infertile men with normal karyotypes and low sperm concentrations and/or less motile spermatozoa have significantly increased risks of producing aneuploid (diminished mature) spermatozoa. Selecting spermatozoa by hyaluronic acid (HA) binding, based on a binding between sperm receptors for zona pellucida and HA, may reduce the potential genetic risks such as chromosomal rearrangements and numerical aberrations. In the present study we examined sperm samples of 45 men with different sperm parameters by HA-binding assay and fluorescence in-situ hybridization (FISH). Mean percentage of HA-bound spermatozoa in the normozoospermic group was significantly higher than the oligozoospermic, the asthenozoospermic and the oligoasthenozoospermic groups. Using FISH, disomy of sex chromosomes and chromosome 17, diploidy and estimated numerical chromosome aberration frequencies were significantly higher in the oligoasthenozoospermic group compared with the three other groups. A significant positive correlation was found between the sperm concentration and the HA-binding capacity, and significant negative correlations between the sperm concentration and the estimated numerical chromosomes aberrations as well as between the HA-binding ability and the estimated numerical chromosome aberrations were identified. We conclude that HA-binding assay and sperm aneuploidy study using FISH may help to predict the reproductive ability of selected infertile male patients and to provide appropriate genetic counselling.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
lissencephaly
subcortical band heterotopia
LIS1 (PAFAH1B1)
DCX
TUBA1A
pachygyria
agyria
egyetemen (Magyarországon) készült közlemény
Megjelenés:Reproductive Biomedicine Online. - 25 : 6 (2012), p. 620-626. -
További szerzők:Molnár Zsuzsanna (1980-) (szülész-nőgyógyász, labor szakorvosjelölt, urológus) Ujfalusi Anikó (1968-) (gyermekorvos, laboratóriumi szakorvos) Balogh Erzsébet (1949-) (biológus, citogenetikus) Bazsáné Kassai Zsuzsa (1946-) (embriológus) Varga Attila (1949-) (urológus, andrológus) Jakab Attila (1964-) (szülész-nőgyógyász, endokrinológus) Oláh Éva (1943-2019) (gyermekgyógyász, klinikai genetikus)
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6.

001-es BibID:BIBFORM075900
Első szerző:Molnár Zsuzsanna (szülész-nőgyógyász, labor szakorvosjelölt, urológus)
Cím:Semen characteristics, fertilization potential, aneuploidy and sperm chromatin decondensation in men with testicular cancer / Molnar Z., Benyo M., Mokanszki A., Kassai Z., Bhattoa H. P., Kiss Z., Jakab A.
Dátum:2015
ISSN:1569-9056
Tárgyszavak:Orvostudományok Klinikai orvostudományok idézhető absztrakt
Megjelenés:European Urology Supplements. - 14 : 2 (2015), p. e298. -
További szerzők:Benyó Mátyás (1982-) (urológus) Mokánszki Attila (1983-) (molekuláris biológus Ph.D hallgató) Bazsáné Kassai Zsuzsa (1946-) (embriológus) Bhattoa Harjit Pal (1973-) (laboratóriumi szakorvos) Kiss Zoltán (1985-) (urológus) Jakab Attila (1964-) (szülész-nőgyógyász, endokrinológus)
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7.

001-es BibID:BIBFORM053794
Első szerző:Molnár Zsuzsanna (szülész-nőgyógyász, labor szakorvosjelölt, urológus)
Cím:Sperm fertilization potential and chromosomal anomalies in testicular cancer before chemotherapy / Z. Molnár, A. Mokánszki, M. Benyó, Z. Bazsáné Kassai, E. Oláh, A. Jakab
Dátum:2011
ISSN:0268-1161
Tárgyszavak:Orvostudományok Klinikai orvostudományok idézhető absztrakt
kemoterápia
spermium genetikai vizsgálat
Megjelenés:Human Reproduction. - 26 (2011), p. i253. -
További szerzők:Mokánszki Attila (1983-) (molekuláris biológus Ph.D hallgató) Benyó Mátyás (1982-) (urológus) Bazsáné Kassai Zsuzsa (1946-) (embriológus) Oláh Éva (1943-2019) (gyermekgyógyász, klinikai genetikus) Jakab Attila (1964-) (szülész-nőgyógyász, endokrinológus)
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8.

001-es BibID:BIBFORM053774
Első szerző:Molnár Zsuzsanna (szülész-nőgyógyász, labor szakorvosjelölt, urológus)
Cím:Sperm concentration, hyaluronic acid binding capacity, aneuploidy and persistent histones in testicular cancer / Z. Molnár, A. Mokanszki, Z. Kassai Bazsáné, H. P. Bhattoa, M. Benyó, É. Oláh, A. Jakab
Dátum:2014
ISSN:0268-1161
Megjegyzések:AbstractStudy question: Are the quantitative and qualitative characteristics of semen samples of patients with testicular cancer (TC), prior to anticancer therapy, different from infertile oligozoospermic and normozoospermic age-matched men?Summary answer: Sperm concentration in TC patients was significantly decreased with no difference in estimated numerical chromosome aberrations and nuclear decondensation compared to normozoospermic men, while the infertile, oligozoospermic men had significantly poorer sperm qualitative characteristics versus the TC group overall and oligozoospermic patients with testicular cancer.What is known already: Spermatogenesis is altered in TC patients at the time of the diagnosis. However, the mechanism responsible for the decreased semen quantity in patients with testicular cancer is not well understood. Anticancer treatment may have gonadotoxic side effects and post treatment fertility cannot be predicted. Before commencing anticancer treatment, cryopreservation may be suggested to preserve fertility but there are no data regarding the risk of genetic aberrations in these sperms.Study design, size, duration: This is a cross-sectional study examining semen from 28 patients with testicular cancer, 20 oligozoospermic and 20 normozoospermic age-matched men attending the Andrology Center and the Sperm Cryopreservation Laboratory of the Medical and Health Science Center, University of Debrecen. Semen samples from patients with testicular cancer were collected after orchidectomy, but prior to anticancer treatment. Semen samples from TC patients recruited over a period of 4 years were studied. Based on their sperm concentration, TC patients were subgrouped into an oligozoospermic TC (TCO) and a normozoospermic TC (TCN) group. For statistical analysis, the normal group (NZ+IO) comprised non-tumorous normozoospermic (NZ) and infertile oligozoospermic (IO) men.Participants/materials/setting, method: The ejaculates were assessed as per World Health Organization guidelines. Hyaluronic acid (HA) binding capacity was the functional test. To determine the numerical chromosome aberrations we used multicolour fluorescence in-situ hybridization. Aniline blue (AB) staining was performed as a nuclear decondensation marker test. Main results and the role of chance: The results did not reveal any significant difference in disomy of sex chromosomes and chromosome 17, diploidy and estimated numerical chromosome aberrations and AB staining results upon comparing the normozoospermic and testicular cancer groups, although the sperm concentration (p<0.001) and HA binding capacity (p<0.001) were lower in the TC group. Estimated numerical chromosome aberrations (p<0.001), AB staining (p<0.001) and HA-binding capacity (p=0.019) was lower in the infertile, oligozoospermic group as compared to the patients with testicular cancer. The TCO group had significantly better results in every examined parameter than the infertile, oligozoospermic group. In the non-tumorous control group (NZ+IO), a significant (p<0.001) correlation (Spearman's rho=r) was found between sperm concentration and aneuploidy rate (r=-0.642), AB staining (r=-0.876) and HA binding (r=0.842); the HA-binding capacity was related to the aneuploidy rate (r=-0.678) and the AB staining (r=-0.811,); and there was significant correlation between aneuploidy and AB staining (r=0.559). In the TC group, apart from the negative correlation between sperm concentration and estimated chromosomal aberrations (r=-0.642), no other correlations were observed.Limitations, reasons for caution: Data on confounders influencing sperm characteristics, such as smoking, occupational or environmental hazards, alcoholism, co-morbidities and other andrological conditions, were not collected. Wider implications of the findings: This is the first study to demonstrate that sperm qualitative characteristics in anticancer therapy naïve oligozoospermic TC patients differ significantly from those in infertile oligozoospermic men and do not differ from those in normozoospermic men. Our results need to be validated in similar groups of men and in other patient groups with cancer where cryopreservation is advisable. Study funding/competing interest(s): This research was supported by the European Union and the State of Hungary, co-financed by the European Social Fund in the framework of TÁMOP-4.2.4.A/2-11/1-2012-0001 ♭National Excellence Program'. The authors have no conflict of interest to declare.Key words: testicular cancer, infertility, hyaluronic acid binding assay, aniline-blue staining, human sperm fluorescence in-situ hybridization
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
testicular cancer
infertility
hyaluronic acid binding assay
aniline-blue staining
human sperm fluorescence in-situ hybridization
Megjelenés:Human Reproduction. - 29 : 9 (2014), p. 1866-1874. -
További szerzők:Mokánszki Attila (1983-) (molekuláris biológus Ph.D hallgató) Bazsáné Kassai Zsuzsa (1946-) (embriológus) Bhattoa Harjit Pal (1973-) (laboratóriumi szakorvos) Benyó Mátyás (1982-) (urológus) Oláh Éva (1943-2019) (gyermekgyógyász, klinikai genetikus) Jakab Attila (1964-) (szülész-nőgyógyász, endokrinológus)
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