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001-es BibID:BIBFORM077455
Első szerző:Mokánszki Attila (molekuláris biológus Ph.D hallgató)
Cím:Altered microRNAs expression levels of sperm and seminal plasma in patients with infertile ejaculates compared with normozoospermic males / Mokánszki Attila, Molnár Zsuzsanna, Varga Tóthné Emese, Bodnár Béla, Jakab Attila, Bálint Bálint L., Balogh István
Dátum:2020
ISSN:1464-7273 1742-8149
Megjegyzések:Approximately 15% of couples are unable to conceive after one year of unprotected intercourse. Because sperm can be accessed with ease, it is reasonable to search for non-invasive biomarkers in semen. MicroRNAs are a family of short single-stranded non-coding RNA molecules that are capable of regulating gene expression and causing mRNA degradation. We studied the most common 11 spermatogenesis-related microRNAs expression levels in sperm and seminal plasma from patients with oligozoospermic or asthenozoospermic ejaculates, and in men with normo- zoospermic ejaculates. Five of these miRNAs were significantly upregulated and three were downregulated in infertile males compared to men with normozoospermic ejaculates. A statistic- ally significant negative correlation was found between the sperm concentration and several microRNA expression level (let-7a, miR-7-1-3p, miR-141, miR-200a, and miR-429, p < 0.0001) both in sperm and in seminal plasma. We also found positive correlation between sperm concentra- tion and some miRNA expression levels (miR-15b, miR-34b, and miR-122, p<0.001) in sperm and in seminal plasma. This is the first study to demonstrate differences between sperm and seminal plasma miRNA expression level and to identify a correlation between the sperm concen- tration and miRNAs expression level. Therefore, these MiRNAs could have the potential be used as non-invasive biomarkers to diagnose males with impaired sperm production.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
male infertility
sperm
microRNA
gene expression
Megjelenés:Human Fertility. - 23 : 4 (2020), p. 246-255. -
További szerzők:Molnár Zsuzsanna (1980-) (szülész-nőgyógyász, labor szakorvosjelölt, urológus) Tóthné Varga Emese Bodnár Béla Jakab Attila (1964-) (szülész-nőgyógyász, endokrinológus) Bálint Bálint László (1971-) (kutató orvos) Balogh István (1972-) (molekuláris biológus, genetikus)
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001-es BibID:BIBFORM055581
Első szerző:Mokánszki Attila (molekuláris biológus Ph.D hallgató)
Cím:Is sperm hyaluronic acid binding ability predictive for clinical success of intracytoplasmic sperm injection : PICSI vs. ICSI? / Attila Mokánszki, Emese Tóthné Varga, Béla Bodnár, Zoltán Tándor, Zsuzsanna Molnár, Attila Jakab, Anikó Ujfalusi, Éva Oláh
Dátum:2014
ISSN:1939-6368 1939-6376
Megjegyzések:Although intracytoplasmic sperm injection (ICSI) is now a widely-used technique, it is still of interest to improve our knowledge as to which is the best spermatozoon to be selected for ICSI. Infertile men have increased risks of producing aneuploid spermatozoa. Using hyaluronic acid (HA)-binding sperm selection may reduce the genetic risks such as chromosomal aberrations of offspring. In the present study we examined the clinical success of ICSI with HA-selected sperm (♭physiologic' ICSI, PICSI) compared to conventional ICSI, as well as the necessity to differentiate patients according to the initial HA-binding assay result (HBA score) and whether the sperm concentration or HBA score can provide additional information. We observed a significantly higher fertilization rate (FR) of the PICSI group with 460% HBA, implantation rate (IR) of the PICSI group with ??60% HBA, and clinical pregnanacy rate (CPR) in every PICSI group compared to the ICSI groups (p50.01). We also observed a significantly higher life birth rate (LBR) in the PICSI group with ??60% HBA compared to ICSI patients with ??60% HBA (p50.001). The pregnancy loss rate (PLR) was significanly lower in PICSI patients compared to the ICSI group (p50.0001). The FR, IR, CPR, and LBR of the PICSI group with 550% HBA were significantly higher and the PLR was lower than in the ICSI group with 550% HBA (p50.01). A statistically significant correlation was found between the sperm concentration and the HA-binding capacity (r1?40.62, p50.001). We found a closer relationship between HBA score and FR (r 1?4 0.53, NS) than between sperm concentration and FR (r 1?4 0.14, NS). HBA could be considered for sperm selection prior to ICSI because of its success and apparant ability to reduce genetic complications. However, this must be extended to a larger study.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Clinical success
hyaluronic acid (HA)-binding capacity (HBA score)
intracytoplasmic sperm injection (ICSI)
♭physiologic' ICSI (PICSI)
sperm concentration
Megjelenés:Systems Biology in Reproductive Medicine 60 : 6 (2014), p. 348-354. -
További szerzők:Tóthné Varga Emese Bodnár Béla Tándor Zoltán (szülész-nőgyógyász) Molnár Zsuzsanna (1980-) (szülész-nőgyógyász, labor szakorvosjelölt, urológus) Jakab Attila (1964-) (szülész-nőgyógyász, endokrinológus) Ujfalusi Anikó (1968-) (gyermekorvos, laboratóriumi szakorvos) Oláh Éva (1943-2019) (gyermekgyógyász, klinikai genetikus)
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