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001-es BibID:	BIBFORM067950
035-os BibID:	(cikkazonosító)e0174585 (WOS)000399116700034 (Scopus)85016151227
Első szerző:	Chen, Ji-Qing
Cím:	MicroRNA expression profiles identify disease-specific alterations in systemic lupus erythematosus and primary Sjögren's syndrome / Ji-Qing Chen, Gábor Papp, Szilárd Póliska, Krisztina Szabó, Tünde Tarr, Bálint László Bálint, Péter Szodoray, Margit Zeher
Dátum:	2017
ISSN:	1932-6203
Megjegyzések:	The discovery of microRNAs (miRNAs) and their critical role in genetic control opened new avenues in understanding of various biological processes including immune cell lineage commitment, differentiation, proliferation and apoptosis. However, a given miRNA may have hundreds of different mRNA targets and a target might be regulated by multiple miRNAs, thus the characterisation of dysregulated miRNA expression profiles could give a better insight into the development of immunological disturbances in autoimmune diseases. The aim of our study was to examine the changes in miRNA expression profiles in patients with systemic lupus erythematosus (SLE) and primary Sjögren's syndrome (pSS). Eight SLE patients, 8 pSS patients and 7 healthy subjects were enrolled in the investigation. MiRNAs were isolated from peripheral blood mononuclear cells, and expression patterns were determined with Illumina next-generation sequencing technology. Since the immunopathogenesis of pSS and SLE encompasses pronounced B cell hyperactivity along with specific autoantibody production, we paid a special attention on the association between miRNA expression levels and altered peripheral B cell distribution. In SLE patients 135, while in pSS patients 26 miRNAs showed altered expression. Interestingly, the 25 miRNAs including miR-146a, miR-16 and miR-21, which were over-expressed in pSS patients, were found to be elevated in SLE group, as well. On the contrary, we observed the down-regulation of miR-150-5p, which is a novel and unique finding in pSS. Levels of several miRNAs over-expressed in SLE, were not changed in pSS, such as miR-148a-3p, miR-152, miR-155, miR-223, miR-224, miR-326 and miR-342. Expression levels of miR-223-5p, miR-150-5p, miR-155-5p and miR-342-3p, which miRNAs are potentially linked to B cell functions, showed associations with the B cell proportions within peripheral blood mononuclear cells. The observed differences in miRNA expression profiles and the better understanding of immune regulatory mechanisms of miRNAs may help to elucidate the pathogenesis of SLE and pSS.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk MicroRNA systemic lupus erythematosus primary Sjögren's syndrome
Megjelenés:	PloS One. - 12 : 3 (2017), p. 1-32. -
További szerzők:	Papp Gábor (1984-) (belgyógyász) Póliska Szilárd (1978-) (biológus) Szabó Krisztina (1987-) (Molekuláris biológus) Tarr Tünde (1976-) (belgyógyász, allergológus és klinikai immunológus) Bálint Bálint László (1971-) (kutató orvos) Szodoray Péter (1973-) (belgyógyász, orvos) Zeher Margit (1957-2018) (belgyógyász, allergológus és klinikai immunológus, reumatológus)
Pályázati támogatás:	K-101470 OTKA ÚNKP-16-4-III Egyéb
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat DOI
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001-es BibID:	BIBFORM041944
Első szerző:	Pázmándi Kitti Linda (molekuláris biológus, immunológus)
Cím:	Ragweed subpollen particles of respirable size activate human dendritic cells / Pazmandi K., Kumar B. V., Szabo K., Boldogh I., Szoor A., Vereb G., Veres A., Lanyi A., Rajnavolgyi E., Bacsi A.
Dátum:	2012
ISSN:	1932-6203
Megjegyzések:	Ragweed (Ambrosia artemisiifolia) pollen grains, which are generally considered too large to reach the lower respiratory tract, release subpollen particles (SPPs) of respirable size upon hydration. These SPPs contain allergenic proteins and functional NAD(P)H oxidases. In this study, we examined whether exposure to SPPs initiates the activation of human monocyte-derived dendritic cells (moDCs). We found that treatment with freshly isolated ragweed SPPs increased the intracellular levels of reactive oxygen species (ROS) in moDCs. Phagocytosis of SPPs by moDCs, as demonstrated by confocal laser-scanning microscopy, led to an up-regulation of the cell surface expression of CD40, CD80, CD86, and HLA-DQ and an increase in the production of IL-6, TNF-?, IL-8, and IL-10. Furthermore, SPP-treated moDCs had an increased capacity to stimulate the proliferation of naïve T cells. Co-culture of SPP-treated moDCs with allogeneic CD3(+) pan-T cells resulted in increased secretion of IFN-? and IL-17 by T cells of both allergic and non-allergic subjects, but induced the production of IL-4 exclusively from the T cells of allergic individuals. Addition of exogenous NADPH further increased, while heat-inactivation or pre-treatment with diphenyleneiodonium (DPI), an inhibitor of NADPH oxidases, strongly diminished, the ability of SPPs to induce phenotypic and functional changes in moDCs, indicating that these processes were mediated, at least partly, by the intrinsic NAD(P)H oxidase activity of SPPs. Collectively, our data suggest that inhaled ragweed SPPs are fully capable of activating dendritic cells (DCs) in the airways and SPPs' NAD(P)H oxidase activity is involved in initiation of adaptive immune responses against innocuous pollen proteins.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Doktori iskola Molekuláris Medicina Open Access
Megjelenés:	Plos One. - 7 : 12 (2012), p. e52085. -
További szerzők:	Kumar, Brahma V. Szabó Krisztina (1987-) (Molekuláris biológus) Boldogh István Szöőr Árpád (1984-) (orvos) Vereb György (1965-) (biofizikus, orvos) Veres Ágota (laboráns) Lányi Árpád (1962-) (biológus, immunológus) Rajnavölgyi Éva (1950-) (immunológus) Bácsi Attila (1967-) (immunológus)
Pályázati támogatás:	TÁMOP-4.2.2/B-10/1-2010-0024 TÁMOP Molekuláris Sejt- és Immunbiológiai Doktori Iskola TÁMOP-4.2.1/B-09/1/KONV-2010-0007 TÁMOP Az oxidatív DNS károsodások javítása és a gyulladás kialakulásának kapcsolata TÁMOP-4.2.1/B-09/1/KONV-2010-0007 TÁMOP Jelátviteli kapcsolatok ős- és dendritikus sejt altípusokban Bolyai János Ösztöndíj MTA DE OEC Bridging Fund Egyéb K 73347 OTKA NK 101538 OTKA
Internet cím:	DOI Intézményi repozitóriumban (DEA) tárolt változat
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