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1.

001-es BibID:BIBFORM070339
Első szerző:Gangl, Helmut
Cím:Influence of artificial interspecies yeast hybrids and their F1 offspring on the aroma profile of wine / Helmut Gangl, Wolfgang Tiefenbrunner, Walter P. Pfliegler, Matthias Sipiczki, Gerhard Leitner, Gabriele Tscheik, Ksenija Lopandic
Dátum:2017
ISSN:0250-4944
Megjegyzések:In the present study we describe the fermentation characteristics of some novel, artifi cial yeast hybrids of three Saccharomycesspecies, two of them (S. cerevisiae and S. uvarum) adapted to high osmotic, high alcohol conditions. Th enon-commercial parental strains, hybrids (S. cerevisiae x S. uvarum, S. cerevisiae x S. kudriavzevii, S. kudriavzevii x S.uvarum) and meiotic segregants (F1 off spring) were utilized for fermentation of high- and low-osmotic grape juice,the dynamics of which was observed and the aroma profi le of all resulting wines was compared to discover whethersome of the hybrids or segregants have favourable properties concerning wine fl avour and taste. The analyzed artifi cialhybrids of the Saccharomyces yeast strains had promising properties for production of high-quality wine: good glucoseand fructose utilization even under high-alcohol conditions, high fermentation rates and generally a pronounced metabolicpower. Th e latt er is precondition to high-level aroma constituent generation during fermentation. Dependingon osmotic stress aroma profi les were very diverse but hybrids outperformed their parental yeast strains under bothconditions, probably because they could use more metabolic pathways and maybe produced even more enzymes andto a higher quantity. Th is may hold for some of the segregants, too, which reached a high quality level concerning wineproduction. Genetic similarity patt ern of the hybrids was not resembled by the one of wine characters (fermentationdynamics and aroma profi le), the main reason of the discrepancy is the higher metabolic power of hybrids.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Saccharomyces cerevisiae
Saccharomyces uvarum
Saccharomyces kudriavzevii
artifi cial hybrids
segregants
hybrid evolution
juice, wine
aroma profile
Megjelenés:Mitteilungen Klosterneuburg 67 (2017), p. 68-83. -
További szerzők:Tiefenbrunner, Wolfgang Pfliegler Valter Péter (1986-) (molekuláris biológus) Sipiczki Mátyás (1948-) (biológus) Leitner, Gerhard Tscheik, Gabriele Lopandic, Ksenija
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2.

001-es BibID:BIBFORM076273
035-os BibID:(WoS)000456491100101 (Scopus)85057718020
Első szerző:Kállai Zoltán (biológus)
Cím:Preservation of diversity and oenological properties of wine yeasts during long-term laboratory maintenance: A study of strains of a century-old Tokaj wine yeast collection / Kállai Zoltán, Pfliegler Walter P., Mitercsák Judit, Szendei Gergő, Sipiczki Matthias
Dátum:2019
ISSN:0023-6438
Megjegyzések:The effect of long-term laboratory maintenance on the biological diversity and certain oenolological properties of wine yeasts was investigated by the analysis of 17 strains isolated from Tokaj (Hungary) wines more than a century ago and maintained since then in culture collections. The analysis of the D1/D2 domains of the large subunit ribosomal RNA genes and the ITS sequences assigned them all to S. cerevisiae but divided them into two groups. One group showed sequence identity with wine strains occurring mainly in countries of the region. The combined results of the interdelta, RAPD, microsatellite-primed PCR (MSP), mtDNA and karyotype analyses found both groups diverse and defined each strain as unique, demonstrating that the long period of maintenance under identical conditions has not resulted in convergent evolution in their genomes. High diversity was detected also in certain phenotypic traits of oenological relevance (osmotolerance, killer phenotype, production of glycerol, ethanol, H2S and volatile acids). In the microvinification experiments all strains performed better than the laboratory strain S288c and were comparable to the industrial strain used as a control or even surpassed it in certain parameters. Thus, the oenological abilities of wine yeasts can be preserved over long periods of maintenance under laboratory conditions.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Yeast
Wine
Diversity
Fermentation
Taxonomy
Megjelenés:Lwt-Food Science And Technology. - 101 (2019), p. 789-798. -
További szerzők:Pfliegler Valter Péter (1986-) (molekuláris biológus) Mitercsák Judit Szendei Gergő Sipiczki Mátyás (1948-) (biológus)
Pályázati támogatás:124417
OTKA
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3.

001-es BibID:BIBFORM064290
Első szerző:Lopandic, Ksenija
Cím:Genotypic and phenotypic evolution of yeast interspecies hybrids during high-sugar fermentation / Ksenija Lopandic, Walter P. Pfliegler, Wolfgang Tiefenbrunner, Helmut Gangl, Matthias Sipiczki, Katja Sterflinger
Dátum:2016
ISSN:0175-7598
Megjegyzések:The yeasts of the Saccharomyces genus exhibit a low pre-zygotic barrier and readily form interspecies hybrids. Following the hybridization event, the parental genomes undergo gross chromosomal rearrangements and genome modifications that may markedly influence the metabolic activity of descendants. In the present study, two artificially constructed hybrid yeasts (Saccharomyces cerevisiae x Saccharomyces uvarum and S. cerevisiae x Saccharomyces kudriavzevii) were used in order to evaluate the influence of high-sugar wine fermentation on the evolution of their genotypic and phenotypic properties. It was demonstrated that the extent of genomic modifications differs among the hybrids and their progeny, but that stress should not always be a generator of large genomic disturbances. The major genome changes were observed after meiosis in the F1 segregants in the form of the loss of different non-S. cerevisiae chromosomes. Under fermentation condition, each spore clone from a tetrad developed a mixed population characterized by different genotypic and phenotypic properties. The S. cerevisiae x S. uvarum spore clones revealed large modifications at the sequence level of the S. cerevisiae sub-genome, and some of the clones lost a few additional S. cerevisiae and S. uvarum chromosomes. The S. cerevisiae x S. kudriavzevii segregants were subjected to consecutive loss of the S. kudriavzevii markers and chromosomes. Both the hybrid types showed increased ethanol and glycerol production as well as better sugar consumption than their parental strains. The hybrid segregants responded differently to stress and a correlation was found between the observed genotypes and fermentation performances.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Applied Microbiology And Biotechnology. - 100 : 14 (2016), p. 6331-6343. -
További szerzők:Pfliegler Valter Péter (1986-) (molekuláris biológus) Tiefenbrunner, Wolfgang Gangl, Helmut Sipiczki Mátyás (1948-) (biológus) Sterflinger, Katja
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4.

001-es BibID:BIBFORM070028
Első szerző:Pfliegler Valter Péter (molekuláris biológus)
Cím:Commercial strain-derived clinical Saccharomyces cerevisiae can evolve new phenotypes without higher pathogenicity / Walter P. Pfliegler, Enikő Boros, Kitti Pázmándi, Ágnes Jakab, Imre Zsuga, Renátó Kovács, Edit Urbán, Zsuzsa Antunovics, Attila Bácsi, Matthias Sipiczki, László Majoros, István Pócsi
Dátum:2017
ISSN:1613-4125
Megjegyzések:Scope: Saccharomyces cerevisiae is one of the most important microbes in food industry, but there is growing evidence on its potential pathogenicity as well. Its status as a member of human mycobiome is still not fully understood.Methods and results: In this study, we characterise clinical S. cerevisiae isolates from Hungarian hospitals along with commercial baking and probiotic strains, and determine their phenotypic parameters, virulence factors, interactions with human macrophages, and pathogenicity. Four of the clinical isolates could be traced back to commercial strains based on genetic fingerprinting. Our observations indicate that the commercial-derived clinical isolates have evolved new phenotypes and show similar, or in two cases, significantly decreased pathogenicity. Furthermore, immunological experiments revealed that the variability in human primary macrophage activation after co-incubation with yeasts is largely donor- and not isolate-dependent.Conclusion: Isolates in this study offer an interesting insight into the potential microevolution of probiotic and food strains in human hosts. These commensal yeasts display various changes in their phenotypes, indicating that the colonization of the host does not necessarily impose a selective pressure towards higher virulence/pathogenicity.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
mycobiom
baking yeast
commensal
microevolution
probiotic
Megjelenés:Molecular Nutrition & Food Research 61 : 11 (2017), p. 1601099-1 - 1601099-8. -
További szerzők:Boros Enikő (1989-) (molekuláris biológus) Pázmándi Kitti Linda (1984-) (molekuláris biológus, immunológus) Jakab Ágnes (1987-) (biológus) Zsuga Imre Kovács Renátó László (1987-) (molekuláris biológus) Urbán Edit Antunovics Zsuzsa (1978-) (molekuláris biológus, genetikus) Bácsi Attila (1967-) (immunológus) Sipiczki Mátyás (1948-) (biológus) Majoros László (1966-) (szakorvos, klinikai mikrobiológus) Pócsi István (1961-) (vegyész)
Pályázati támogatás:MTA posztdoktori program
MTA
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5.

001-es BibID:BIBFORM065870
Első szerző:Pfliegler Valter Péter (molekuláris biológus)
Cím:Does fingerprinting truly represent the diversity of wine yeasts? A case study with interdelta genotyping of S. cerevisiae strains / Walter P. Pfliegler, Matthias Sipiczki
Dátum:2016
ISSN:0266-8254 1472-765X
Megjegyzések:Simple and efficient genotyping methods are widely used to assess the diversity of a large number of microbial strains, e.g. wine yeasts isolated from a specific geographic area or a vintage, etc. Such methods are often also the first to be applied, to decrease the number of strains deemed interesting for a more time-consuming physiological characterization. Here, we aimed to use a physiologically characterized strain collection of 69 S. cerevisiae strains from Hungarian wine regions to determine whether geographic origin or physiological similarity can be recovered by clustering the strains with one or two simultaneously used variations of interdelta genotyping. Our results indicate that although a detailed clustering with high resolution can be achieved with this method, the clustering of strains is largely contrasting when different primer sets are used and it does not recover geographic or physiological groups.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Letters In Applied Microbiology 63 : 6 (2016), p. 406-411. -
További szerzők:Sipiczki Mátyás (1948-) (biológus)
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6.

001-es BibID:BIBFORM062589
Első szerző:Pfliegler Valter Péter (molekuláris biológus)
Cím:First record of the fungus Pringsheimia smilacis E. Müller 1958 (Ascomycota: Dothioraceae) from Hungary / Walter P. Pfliegler, Matthias Sipiczki
Dátum:2013
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény hazai lapban
Megjelenés:e-Acta Naturalia Pannonica. - 5 (2013), p. 33-37. -
További szerzők:Sipiczki Mátyás (1948-) (biológus)
Pályázati támogatás:TÁMOP 4.2.4. A/2-11-1-2012-0001
TÁMOP
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7.

001-es BibID:BIBFORM055415
Első szerző:Pfliegler Valter Péter (molekuláris biológus)
Cím:Generation of new genotypic and phenotypic features in artificial and natural yeast hybrids / Walter P. Pfliegler, Lea Atanasova, Edina Karanyicz, Matthias Sipiczki, Ursula Bond, Irina S. Druzhinina, Katja Sterflinger, Ksenija Lopandic
Dátum:2014
ISSN:1330-9862 1334-2606
Megjegyzések:Evolution and genome stabilization have mostly been studied on the Saccharomyceshybrids isolated from natural and alcoholic fermentation environments. Genetic and phenotypicproperties have usually been compared to the laboratory and reference strains, as thetrue ancestors of the natural hybrid yeasts are unknown. In this way the exact impact ofdifferent parental fractions on the genome organization or metabolic activity of the hybridyeasts is difficult to resolve completely. In the present work the evolution of geno- andphenotypic properties is studied in the interspecies hybrids created by the cross-breedingof S. cerevisiae with S. uvarum or S. kudriavzevii auxotrophic mutants. We hypothesized thatthe extent of genomic alterations in S. cerevisiae ? S. uvarum and S. cerevisiae ? S. kudriavzeviishould affect the physiology of their F1 offspring in different ways. Our results, obtainedby amplified fragment length polymorphism (AFLP) genotyping and karyotypinganalyses, showed that both subgenomes of the S. cerevisiae x S. uvarum and of S. cerevisiae ?S. kudriavzevii hybrids experienced various modifications. However, the S. cerevisiae ? S.kudriavzevii F1 hybrids underwent more severe genomic alterations than the S. cerevisiae ?S. uvarum ones. Generation of the new genotypes also influenced the physiological performancesof the hybrids and the occurrence of novel phenotypes. Significant differencesin carbohydrate utilization and distinct growth dynamics at increasing concentrations ofsodium chloride, urea and miconazole were observed within and between the S. cerevisiae? S. uvarum and S. cerevisiae ? S. kudriavzevii hybrids. Parental strains also demonstrateddifferent contributions to the final metabolic outcomes of the hybrid yeasts. A comparisonof the genotypic properties of the artificial hybrids with several hybrid isolates from thewine-related environments and wastewater demonstrated a greater genetic variability ofthe S. cerevisiae ? S. kudriavzevii hybrids. Saccharomyces cerevisiae ? S. uvarum artificial andnatural hybrids showed considerable differences in osmolyte tolerance and sensitivity tomiconazole, whereas the S. cerevisiae ? S. kudriavzevii hybrids exhibited differences also inmaltotriose utilization. The results of this study suggest that chromosomal rearrangementsand genomic reorganizations as post-hybridization processes may affect the phenotypicproperties of the hybrid progeny substantially. Relative to their ancestors, the F1 segregants may generate different phenotypes, indicating novel routes of evolution in responseto environmental growth conditions.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Food Technology and Biotechnology. - 52 : 1 (2014), p. 46-57. -
További szerzők:Atanasova, Lea Karanyicz Edina Sipiczki Mátyás (1948-) (biológus) Bond, Ursula Druzhinina, Irina S. Sterflinger, Katja Lopandic, Ksenija
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8.

001-es BibID:BIBFORM055087
Első szerző:Pfliegler Valter Péter (molekuláris biológus)
Cím:Diversity of Candida zemplinina isolates inferred from RAPD, micro/minisatellite and physiological analysis / Walter P. Pfliegler, Enikő Horváth, Zoltán Kállai, Matthias Sipiczki
Dátum:2014
ISSN:0944-5013
Megjegyzések:Among non-Saccharomyces wine yeasts, Candida zemlpinina is one of the frequently isolated and oenologically important species. It is mostly known from European winemaking areas and it has become one of the key species of non-Saccharomyces wine yeasts to study. Investigating the diversity of C. zemplinina isolates is important for a deeper understanding of the non-Saccharomyces wine yeasts and for the yeast starter industry, as numerous researches have pointed to the potential use of this species in winemaking. For assessing the biodiversity of a larger number of strains, RAPD and micro/minisatellite PCR is often the method of choice, however, this technique is often unstandardized. Whereas some laboratories use these methods for species identifications, others apply RAPD primers for determining intraspecies diversity. In this study, we have tested 5 different RAPD and micro/minisatellite primers on strains of C. zemplinina isolated from different locations. We show that after a rigorous PCR-optimization aimed at reproducibility and comparability of band patterns with these PCR-reactions, diversity of different strains from a wide range of geographic locations is relatively low. The analysis of several oenologically important physiological traits of the strains showed a relatively low level of diversity as well. We also demonstrate that the intraspecific diversity of C. zemplinina observable with different techniques (RAPD, micro/minisatellite or physiological analysis) may be fairly different and not necessarily comparable.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Microbiological Research. - 169 : 5-6 (2014), p. 402-410. -
További szerzők:Horváth Enikő (1987-) (biológus) Kállai Zoltán (1986-) (biológus) Sipiczki Mátyás (1948-) (biológus)
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9.

001-es BibID:BIBFORM043960
Első szerző:Pfliegler Valter Péter (molekuláris biológus)
Cím:Double sterility barrier between Saccharomyces species and its breakdown in allopolyploid hybrids by chromosome loss / Walter P. Pfliegler, Zsuzsa Antunovics, Matthias Sipiczki
Dátum:2012
ISSN:1567-1356
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Fems Yeast Research. - 12 : 6 (2012), p. 703-718. -
További szerzők:Antunovics Zsuzsa (1978-) (molekuláris biológus, genetikus) Sipiczki Mátyás (1948-) (biológus)
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10.

001-es BibID:BIBFORM074051
035-os BibID:(cikkazonosító)1193 (WOS)000434951800001 (Scopus)85048341000
Első szerző:Sipiczki Mátyás (biológus)
Cím:Birth-and-Death Evolution and Reticulation of ITS Segments of Metschnikowia andauensis and Metschnikowia fructicola rDNA Repeats / Sipiczki Matthias, Horvath Eniko, Pfliegler Walter P.
Dátum:2018
ISSN:1664-302X
Megjegyzések:The internal transcribed spacer (ITS) region (ITS1, 5.8S rDNA, and ITS2) separates the genes coding for the SSU 18S and the LSU 26S genes in the rDNA units which are organized into long tandem arrays in the overwhelming majority of fungi. As members of a multigenic family, these units are subject of concerted evolution, which homogenizes their sequences. Exceptions have been observed in certain groups of plants and in a few fungal species. In our previous study we described exceptionally high degree of sequence diversity in the D1/D2 domains of two pulcherrimin-producing Metschnikowia (Saccharomycotina) species which appeared to evolve by reticulation. The major goals of this study were the examination of the diversity of the ITS segments and their evolution. We show that the ITS sequences of these species are not homogenized either, differ from each other by up to 38 substitutions and indels which have dramatic effects on the predicted secondary structures of the transcripts. The high intragenomic diversity makes the D1/D2 domains and the ITS spacers unsuitable for barcoding of these species and therefore the taxonomic position of strains previously assigned to them needs revision. By analyzing the genome sequence of the M. fructicola type strain, we also show that the rDNA of this species is fragmented, contains pseudogenes and thus evolves by the birth-and-death mechanism rather than by homogenisation, which is unusual in yeasts. The results of the network analysis of the sequences further indicate that the ITS regions are also involved in reticulation. M. andauensis and M. fructicola can form interspecies hybrids and their hybrids segregate, providing thus possibilities for reticulation of the rDNA repeats.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
structure prediction
yeast
ribosomal RNA
ITS
evolution
pseudogene
reticulation
hybridisation
Megjelenés:Frontiers in Microbiology. - 9 (2018), p. 1-16. -
További szerzők:Horváth Enikő (1987-) (biológus) Pfliegler Valter Péter (1986-) (molekuláris biológus)
Pályázati támogatás:K-124417
Egyéb
TÁMOP-4.2.4.A/2-11/1-2012-0001
Egyéb
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11.

001-es BibID:BIBFORM064289
Első szerző:Sipiczki Mátyás (biológus)
Cím:Metahyphopichia laotica gen. nov., sp. nov., a polymorphic yeast related to Hyphopichia / Matthias Sipiczki, Walter P. Pfliegler, Silvana V. B. Safar, Paula B. Morais, Carlos A. Rosa
Dátum:2016
ISSN:1466-5026 1466-5034
Megjegyzések:Four strains alternating between yeast and filamentous growth morphologies were isolated from flowers in two regions of Laos. In liquid environment the isolates propagated by budding and developed irregularly shaped pseudohyphae. On solid media their yeast cells switched to hyphal growth which could return to the yeast phase by developing lateral blastoconidia. The sequences of the D1/D2 domains of the large subunit (LSU) 26S rRNA genes, the internal transcribed spacer (ITS) regions and the small subunit (SSU) 18S rRNA genes were identical in the four strains and differed from the corresponding sequences of other yeast species available in databases by at least 11% (D1/D2), 13% (ITS) and 7% (SSU). In an independent project, two strains with D1/D2 and ITS sequences very similar to those of the Laotian strains were found in bark samples collected in Brazil. The six strains also differed from the closest yeast species in physiological properties, indicating that they represented a hitherto undescribed species. The phylogenetic analysis of the D1/D2 sequences, and the concatenated sequences of the SSU rRNA genes, D1/D2 domains of LSU rRNA genes as well as the protein-encoding genes ACT1 and TEF1 placed them close to Hyphopichia. To reflect this position, the novel genus name Metahyphopichia and the novel species name Metahyphopichia laotica are proposed for them. The type strain is 11-1006T (=CBS 13022T = CCY 092-001-001T = NCAIM Y.02126T) isolated in Luang Prabang (Laos). Mycobank registration numbers are MB 808253 (Metahyphopichia) and MB 808254 (M. laotica).
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:International Journal Of Systematic And Evolutionary Microbiology. - 66 (2016), p. 2550-2557. -
További szerzők:Pfliegler Valter Péter (1986-) (molekuláris biológus) Rosa, Carlos A. Safar, Silvana V. B. Morais, Paula B
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12.

001-es BibID:BIBFORM062590
Első szerző:Sipiczki Mátyás (biológus)
Cím:Biodiversity in yeast populations associated with botrytised wine making / Mátyás Sipiczki, Hajnalka Csoma, Zsuzsa Antunovics, Walter Pfliegler
Dátum:2010
ISSN:0250-4944
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Mitteilungen Klosterneuburg. - 60 (2010), p. 387-394. -
További szerzők:Csoma Hajnalka (1979-) (biológus, szőlész-borász) Antunovics Zsuzsa (1978-) (molekuláris biológus, genetikus) Pfliegler Valter Péter (1986-) (molekuláris biológus)
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