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001-es BibID:	BIBFORM054306
Első szerző:	Medgyesi Dávid
Cím:	Functional mapping of the FcγRII binding site on human IgG1 by synthetic peptides / Dávid Medgyesi, Katalin Uray, Krisztina Sallai, Ferenc Hudecz, Gábor Koncz, Jakub Abramson, Israel Pecht, Gabriella Sármay, János Gergely
Dátum:	2004
ISSN:	0014-2980
Megjegyzések:	Receptors specific for the Fc part of IgG (FcgammaR) are expressed by several cell types and play diverse roles in immune responses. Impaired function of the activating and inhibitory FcgammaR may result in autoimmunity. Thus, the modulation of IgG-FcgammaR interaction can be a target for the development of treatments for some autoimmune and inflammatory diseases. This study addresses the localization and functional characterization of linear sequences in human IgG1 which bind to FcgammaRII. Peptides with overlapping sequences derived from the CH2 domain of human IgG1 between P(234) and S(298) were synthesized and used in binding and functional experiments. Binding of the peptides to FcgammaR was assayed in vitro and ex vivo, and peptides found to interact were functionally tested. The shortest effective peptide was T(216)-p(271) which bound to soluble recombinant Fc-gammaRIIb with K(d)=6x10(6) M(-1). The biotinylated peptides R(255)-p(271) and T(256)-p(271) complexed by avidin exhibited functional activity; they induced FcgammaRIIb-mediated inhibition of the BCR-triggered Ca(2+) response of human Burkitt lymphoma cells, and inflammatory cytokine production (TNF-alpha and IL-6) by the human monocyte cell line MonoMac. In conclusion, our results suggest that the selected peptides functionally represent the Fc-gammaRII-binding part of IgG1.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban IgG Fc gamma Re fc peptide binding site
Megjelenés:	European Journal of Immunology. - 34 : 4 (2004), p. 1127-1135. -
További szerzők:	Uray Katalin Sallai Krisztina Hudecz Ferenc Koncz Gábor (1970-) (biológus, immunológus) Abramson, Jakub Pecht, Israel Sármay Gabriella Gergely János
Pályázati támogatás:	AKP 2000-40 3,3 MTA
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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