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001-es BibID:	BIBFORM068928
Első szerző:	Murnyák Balázs (molekuláris biológus, genetikus)
Cím:	PARP1 expression and its correlation with survival is tumour molecular subtype dependent in glioblastoma / Balázs Murnyák, Mahan C. Kouhsari, Rotem Hershkovitch, Bernadette Kálmán, György Marko-Varga, Álmos Klekner, Tibor Hortobágyi
Dátum:	2017
ISSN:	1949-2553
Megjegyzések:	Overexpression of PARP1 exists in various cancers, including glioblastoma (GBM). Although PARP1 inhibition is a promising therapeutic target, no comprehensive study has addressed PARP1's expression characteristics and prognostic role regarding molecular heterogeneity in astrocytomas including GBM. Our aim was to evaluate PARP1's associations with survival, WHO grade, lineage specific markers, and GBM transcriptomic subtypes. We collected genomic and clinical data from the latest glioma datasets of The Cancer Genome Atlas and performed PARP1, ATRX, IDH1, and p53 immunohistochemistry on GBM tissue samples. We demonstrated that PARP1 gain and increased mRNA expression are characteristics of high-grade astrocytomas, particularly of Proneural and Classical GBM subtypes. Additionally, higher PARP1 levels exhibited an inverse correlation with patient survival (p<0.005) in the Classical subgroup. ATRX (p=0.006), and TP53 (p=0.015) mutations were associated with increased PARP1 expression and PARP1 protein level correlated with ATRX loss and p53 overexpression. Furthermore, higher PARP1 expression together with wildtype TP53 indicated shorter survival (p=0.039). Therefore, due to subtype specificity, PARP1 expression level and TP53 mutation status are reliable marker candidates to distinguish Proneural and Classical subtypes, with prognostic and therapeutic implications in GBM.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban glioma glioblastoma p53 PARP1
Megjelenés:	Oncotarget. - 8 : 28 (2017), p. 46348-46362. -
További szerzők:	Kouhsari, Mahan C. Hershkovitch, Rotem Kálmán Bernadette Marko-Varga György Klekner Álmos (1970-) (idegsebész) Hortobágyi Tibor (1965-) (patológus)
Pályázati támogatás:	ÚNKP-16-3 Egyéb KTIA_13_NAP-A-II/7 Egyéb KTIA_13_NAP-A-V/3 Egyéb AGR_PIAC_13-1-2013-0008 Egyéb GINOP-2.3.2-15-2016-00043 GINOP
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat DOI
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001-es BibID:	BIBFORM070644
035-os BibID:	(WOS)000456915500003 (Scopus)85029807859
Első szerző:	Nagy Ádám
Cím:	Molecular Subgroups of Glioblastoma : an Assessment by Immunohistochemical Markers / Nagy Ádám, Garzuly Ferenc, Padányi Gergely, Szűcs Iván, Feldmann Ádám, Murnyák Balázs, Hortobágyi Tibor, Kálmán Bernadette
Dátum:	2019
ISSN:	1219-4956
Megjegyzések:	Comprehensive molecular characterization of and novel therapeutic approaches to glioblastoma have been explored as a result of advancements in biotechnologies. In this study, we aimed to bring basic research discoveries closer to clinical practice and ultimately incorporate molecular classification into the routine histopathological evaluation of grade IV gliomas. Integrated results of genome-wide sequencing, transcriptomic and epigenomic analyses by The Cancer Genome Atlas Network defined the classic, proneural, neural and mesenchymal subtypes of this tumor. In a retrospective cohort, we analyzed selected subgroup-defining molecular markers in formalin-fixed paraffin-embedded surgical specimens by immunohistochemistry. Quantitative and qualitative scores of marker expression were tested in hierarchical cluster analyses to evaluate segregations of the molecular subgroups, which then were correlated with clinical parameters including patients' age, gender and overall survival. Our study has confirmed the separation of molecular glioblastoma subgroups with clear trends regarding clinical correlations. Future analyses in a larger, prospective cohort using similar methods are expected to facilitate the development of a molecular diagnostic panel that may complement routine histological work up and support prognostication as well as treatment decisions in glioblastoma.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk Glioblastoma Molecular subgroups Translation Clinical setting
Megjelenés:	Pathology & Oncology Research. - 25 : 1 (2019), p. 21-31. -
További szerzők:	Garzuly Ferenc (1937-) (neurológus) Padányi Gergely Szűcs Iván Feldmann Ádám Murnyák Balázs (1986-) (molekuláris biológus, genetikus) Hortobágyi Tibor (1965-) (patológus) Kálmán Bernadette
Pályázati támogatás:	NAP KTIA_13_NAP-A-II/7 KTIA GINOP-2.3.2-15-2016-00043 GINOP
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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