Találati lista | Tudóstér

001-es BibID:	BIBFORM101096
Első szerző:	Dobránszki Judit (biológus)
Cím:	Transcription Profile of Potato (Solanum tuberosum L.) Growing In Vitro / Judit Dobránszki, Norbert Hidvégi, Andrea Gulyás, Bianka Tóth, Jaime A. Teixeira da Silva
Dátum:	2021
ISSN:	0721-7595
Tárgyszavak:	Agrártudományok Növénytermesztési és kertészeti tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk
Megjelenés:	Journal Of Plant Growth Regulation. - 40 : 2 (2021), p. 749-760. -
További szerzők:	Hidvégi Norbert (1986-) Gulyás Andrea (1985-) Tóth Bianka (1988-) (biológus) Teixeira da Silva, Jaime A.
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
Borító:
Saját polcon:

001-es BibID:	bibEBI00026258
Első szerző:	Dobránszki Judit (biológus)
Cím:	Abiotic stress elements in in vitro potato (Solanum tuberosum L.) exposed to air-based and liquid-based ultrasound: A comparative transcriptomic assessment / Judit Dobránszki, Norbert Hidvégi, Andrea Gulyás, Bianka Tóth, Jaime A. Teixeira da Silva
Dátum:	2020
ISSN:	0079-6107
Megjegyzések:	Ultrasound (US) can modify the plant growth and development. Previous assessments of the transcriptome of in vitro potato (Solanum tuberosum L.) exposed to US transmitted through air (AB-US) or liquid (PE-US) revealed the up- or down-regulation of several stress-related differentially expressed genes (DEGs) related to abiotic stress. In a bid to better characterize stress-related elements over a four-week period, the transcriptome of AB-US was compared to that of PE-US. When comparing the controls of both treatments, DEGs related to hypoxia were not detected. Nevertheless, hypoxia-related DEGs were detected in the combination of liquid medium and ultrasonication. DEGs coding for chitinase, peroxidase, glutathione-S-transferase, transcription factors of ERF (ethylene responsive factor), DREB (dehydration-responsive element-binding), WRKY and MYB were also significantly highly expressed in PE-US, relative to AB-US. Up- and down-regulation of DEGs related to metabolic processes, and enzymes of the antioxidant system also confirm that PE-US is a more acute abiotic stress than AB-US.
Tárgyszavak:	Agrártudományok Növénytermesztési és kertészeti tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk Abiotic stress Differentially expressed genes Heat shock proteins Plant growth
Megjelenés:	Progress In Biophysics & Molecular Biology. - 158 (2020), p. 47-56. -
További szerzők:	Hidvégi Norbert (1986-) Gulyás Andrea (1985-) Tóth Bianka (1988-) (biológus) Teixeira da Silva, Jaime A.
Pályázati támogatás:	NKFIH-1150-6/2019 Egyéb
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
Borító:
Saját polcon:

001-es BibID:	BIBFORM085313
Első szerző:	Teixeira da Silva, Jaime A.
Cím:	Transcriptomic Response of In Vitro Potato (Solanum tuberosum L.) to Piezoelectric Ultrasound / Jaime A. Teixeira da Silva, Norbert Hidvégi, Andrea Gulyás, Bianka Tóth, Judit Dobránszki
Dátum:	2020
ISSN:	0735-9640
Megjegyzések:	As part of a wider project to assess the impact of ultrasound on in vitro plant growth, this paper aimed to determine whether the application of piezoelectric ultrasound (PE-US) would induce changes to the transcriptome of in vitro potato (Solanum tuberosum L.). After exposing explants (single-node segments with a single leaf) to PE-US (35 kHz; 70 W) for 20 min, the effect of this stressor was determined at 0 h, 24 h, 48 h, 1 week, and 4 weeks to assess the possible immediate and residual effects of PE-US on the potato transcriptome. After mRNA was isolated, bioinformatic processing and analysis of RNA-seq datasets, KEGG analysis revealed that 24% of up-regulated biological processes were a response to stress immediately after the application of PE-US (reducing to 11% and 9% at 48 h and 4 weeks, respectively). After assessing a total of 40,430 genes for expression intensity at these five time intervals, it was found that 138, 72, 18, 5, and 59 differentially expressed genes (DEGs), respectively, were significantly up-regulated, while 6, 82, 96, 172, and 107 DEGs, respectively, were down-regulated. DEGs coding for universal stress protein, chitinase, catalase, zinc finger proteins, 21 transcription factors, glutathione S-transferase, and 17 heat shock proteins, mainly Hsp70, Hsp20, and Hsp90 proteins, and possibly the first of such discovery in US-based plant stress research, was detected. Following the validation of RNA-seq data by RT-qPCR, between SeqMonk LFC and RT-qPCR LFC, the Spearman and Pearson correlation coefficients were 0.86 and 0.90, respectively. Plantlets exposed to PE-US had significantly shorter shoots but significantly longer roots as well as higher shoot and root fresh weight, while chl b and total chl were significantly lower but the chl a/b ratio was significantly higher in plantlets exposed to PE-US. PE-US thus constituted an acute abiotic stress, but by 4 weeks, in vitro potato plantlets managed to mitigate the stress through the production of antioxidant enzyme systems and other functional metabolic changes.
Tárgyszavak:	Agrártudományok Növénytermesztési és kertészeti tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk Abiotic stress Chlorophyll Differentially expressed genes Enzymes Heat shock proteins Plant growth RT-qPCR Wounding
Megjelenés:	Plant Molecular Biology Reporter. - 38 : 3 (2020), p. 404-418. -
További szerzők:	Hidvégi Norbert (1986-) Gulyás Andrea (1985-) Tóth Bianka (1988-) (biológus) Dobránszki Judit (1967-) (biológus)
Pályázati támogatás:	NKFIH-1150-6/2019 Egyéb
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
Borító:
Saját polcon: