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001-es BibID:BIBFORM049213
Első szerző:Brázda Péter (biológus, angol-magyar szakfordító)
Cím:Ligand binding shifts highly mobile RXR to chromatin-bound state in a coactivator-dependent manner as revealed by single cell imaging / Peter Brazda, Jan Krieger, Bence Daniel, David Jonas, Tibor Szekeres, Jörg Langowski, Katalin Tóth, Laszlo Nagy, György Vámosi
Dátum:2014
ISSN:0270-7306
Megjegyzések:Retinoid X Receptor (RXR) is a promiscuous nuclear receptor forming heterodimers with several other receptors, which activate different sets of genes. Upon agonist treatment the occupancy of its genomic binding regions increased, but only a modest change in the number of sites was revealed by ChIP-Seq, suggesting a rather static behavior. However, such genome-wide and biochemical approaches do not take into account the dynamic behavior of a transcription factor. Therefore we characterized the nuclear dynamics of RXR during activation in single cells on the sub-second scale using live-cell imaging. By applying FRAP and fluorescence correlation spectroscopy (FCS), techniques with different temporal and spatial resolution, a highly dynamic behavior could be uncovered, which is best described by a two-state model of receptor mobility. In the unliganded state most RXRs belonged to the fast population, leaving ~15% for the slow, chromatin bound fraction. Upon agonist treatment, this ratio increased to ~43% as a result of an immediate and reversible redistribution. Coactivator binding appears to be indispensable for redistribution and has a major contribution to chromatin association. A nuclear mobility map recorded by light sheet microscopy-FCS shows that the ligand-induced transition from the fast to the slow population occurs throughout the nucleus. Our results support a model in which RXR has a distinct, highly dynamic nuclear behavior and follows hit-and-run kinetics upon activation.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
RXR
dynamics
nuclear receptor
diffusion
transcription
Megjelenés:Molecular and Cellular Biology. - 34 : 7 (2014), p. 1234-1245. -
További szerzők:Krieger, Jan W. Dániel Bence (1987-) (molekuláris biológus) Jonás Dávid Szekeres Tibor (1984-) (molekuláris biológus) Langowski, Jörg Tóth Katalin Ágnes (1977-) (biokémikus, molekuláris biológus) Nagy László (1966-) (molekuláris sejtbiológus, biokémikus) Vámosi György (1967-) (biofizikus)
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
DOI
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2.

001-es BibID:BIBFORM071151
Első szerző:Czimmerer Zsolt (molekuláris biológus)
Cím:Extensive and functional overlap of the STAT6 and RXR cistromes in the active enhancer repertoire of human CD14+ monocyte derived differentiating macrophages / Zsolt Czimmerer, Zsuzsanna S. Nagy, Gergely Nagy, Attila Horvath, Timea Silye-Cseh, Agnes Kriston, David Jonas, Sascha Sauer, Laszlo Steiner, Bence Daniel, Jean-Francois Deleuze, Laszlo Nagy
Dátum:2018
ISSN:0303-7207
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Molecular and Cellular Endocrinology. - 471 (2018), p. 63-74. -
További szerzők:Nagy Zsuzsanna S. Nagy Gergely (1986-) (molekuláris biológus) Horváth Attila (1988-) (programtervező informatikus) Silye-Cseh Timea Kriston Ágnes Jonás Dávid Sauer, Sascha Steiner László Dániel Bence (1987-) (molekuláris biológus) Deleuze, Jean-Francois Nagy László (1966-) (molekuláris sejtbiológus, biokémikus)
Pályázati támogatás:TAMOP 4.2.4. A/2-11-1-2012-0001
TÁMOP
K100196
OTKA
TAMOP-4.2.2/A-11/1/KONV-2012-0023
TÁMOP
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
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3.

001-es BibID:BIBFORM049597
035-os BibID:PMID:23973299 WOS:000325840200014
Első szerző:Nagy Gergely (molekuláris biológus)
Cím:A novel method to predict regulatory regions based on histone mark landscapes in macrophages / Gergely Nagy, Bence Dániel, Dávid Jónás, László Nagy, Endre Barta
Dátum:2013
ISSN:0171-2985
Megjegyzések:Macrophages as phagocytes and professional antigen presenting cells play critical roles in both innate and adaptive immunity. Main transcription factors acting during their differentiation and function are known, but the behavior and co-operation of these factors still remained unexplored. We introduce a new approach to map nucleosome-free regions using exclusively active enhancer and core promoter marking histone modification ChIP-seq data. We could detect approximately 56,000 potential active enhancers/promoters showing different lengths and histone modification shapes. Beside the highly enriched PU.1 and C/EBP sites, we could also detect binding sites for RUNX and AP-1, as well as for the MiT (MITF-TFE) family and MEF2 proteins. The PU.1 and C/EBP transcription factors are known for transforming cells into macrophages. The other transcription factors found in this study can play a role in macrophages as well, since it is known that the MiT family proteins are responsible for phagocytic activity and the MEF2 proteins specify monocytic differentiation over the granulocyte direction. Our results imply that this method can provide novel information about transcription factor organization at enhancers and core promoters as well as about the histone modifications surrounding regulatory regions in any immune or other cell types.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
BWA
Burrows-Wheeler Alignment Tool
CRE
ChIP
DMEM
Dulbecco's modified Eagle's medium
ENCODE
Enhancer
FAIRE
HLH
HOMER
HTH
Histone modification
IGV
Macrophage
NFR
NOR
Nucleosome-free region
PU.1
Promoter
SRA
TSS
bZIP
basic leucine zipper
cAMP response element
chromatin immunoprecipitation
encyclopedia of DNA elements
formaldehyde-assisted isolation of regulatory elements
helix-loop-helix
helix-turn-helix
hypergeometric optimization of motif EnRichment
integrative genomics viewer
nucleosome occupied region
nucleosome-free region
sequence read archive
transcription start site
Megjelenés:Immunobiology. - 218 : 11 (2013), p. 1416-1427. -
További szerzők:Dániel Bence (1987-) (molekuláris biológus) Jonás Dávid Nagy László (1966-) (molekuláris sejtbiológus, biokémikus) Barta Endre (1963-) (molekuláris biológus)
Pályázati támogatás:K100196
OTKA
TÁMOP422_2012_002
TÁMOP
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:
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