CCL

Összesen 1 találat.
#/oldal:
Részletezés:
Rendezés:

1.

001-es BibID:BIBFORM077291
035-os BibID:(WoS)000460412800011 (Scopus)85062658695
Első szerző:Alex, Jimi M.
Cím:Calixarene-mediated assembly of a small antifungal protein / Jimi M. Alex, Martin L. Rennie, Sylvain Engilberge, Gábor Lehoczki, Hajdu Dorottya, Ádám Fizil, Gyula Batta, Peter B. Crowley
Dátum:2019
ISSN:2052-2525
Megjegyzések:Synthetic macrocycles such as calixarenes and cucurbiturils are increasingly applied as mediators of protein assembly and crystallization. The macrocycle can facilitate assembly by providing a surface on which two or more proteins bind simultaneously. This work explores the capacity of the sulfonatocalix[n]arene (sclxn) series to effect crystallization of PAF, a small, cationic antifungal protein. Co-crystallization with sclx4, sclx6 or sclx8 led to highresolution crystal structures. In the absence of sclxn, diffraction-quality crystals of PAF were not obtained. Interestingly, all three sclxn were bound to a similar patch on PAF. The largest and most flexible variant, sclx8, yielded a dimer of PAF. Complex formation was evident in solution via NMR and ITC experiments, showing more pronounced effects with increasing macrocycle size. In agreement with the crystal structure, the ITC data suggested that sclx8 acts as a bidentate ligand. The contributions of calixarene size/conformation to protein recognition and assembly are discussed. Finally, it is suggested that the conserved binding site for anionic calixarenes implicates this region of PAF in membrane binding, which is a prerequisite for antifungal activity.
Tárgyszavak:Természettudományok Kémiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
conformation selection
molecular glue
nucleating agent
polyethylene glycol
supramolecular chemistry
antifungal proteins
calixarene
Megjelenés:IUCrJ. - 6 : 2 (2019), p. 238-247. -
További szerzők:Rennie, Martin L. Engilberge, Sylvain Lehoczki Gábor (1988-) (biológus, molekuláris biológus) Hajdu Dorottya (1987-) (biológus) Fizil Ádám (1988-) (biológus) Batta Gyula (1953-) (molekula-szerkezet kutató) Crowley, Peter B.
Pályázati támogatás:ANN 110821
OTKA
GINOP-2.3.2-15-2016-00008
GINOP
GINOP-2.3.3-15-2016-00004
GINOP
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:
Rekordok letöltése1