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001-es BibID:BIBFORM081867
035-os BibID:(cikkazonosító)e0224936 (WOS)000533881900018 (Scopus)85075250885
Első szerző:Bosire, Rosevalentine (biotechnológus)
Cím:Intercalation of small molecules into DNA in chromatin is primarily controlled by superhelical constraint / Rosevalentine Bosire, Péter Nánási Jr., László Imre, Beatrix Dienes, Árpád Szöőr, Anett Mázló, Attila Kovács, Ralf Seidel, György Vámosi, Gábor Szabó
Dátum:2019
ISSN:1932-6203
Megjegyzések:The restricted access of regulatory factors to their binding sites on DNA wrapped around the nucleosomes is generally interpreted in terms of molecular shielding exerted by nucleosomal structure and internucleosomal interactions. Binding of proteins to DNA often includes intercalation of hydrophobic amino acids into the DNA. To assess the role of constrained superhelicity in limiting these interactions, we studied the binding of small molecule intercalators to chromatin in close to native conditions by laser scanning cytometry. We demonstrate that the nucleosome-constrained superhelical configuration of DNA is the main barrier to intercalation. As a result, intercalating compounds are virtually excluded from the nucleosome-occupied regions of the chromatin. Binding of intercalators to extranucleosomal regions is limited to a smaller degree, in line with the existence of net supercoiling in the regions comprising linker and nucleosome free DNA. Its relaxation by inducing as few as a single nick per ~50 kb increases intercalation in the entire chromatin loop, demonstrating the possibility for long-distance effects of regulatory potential.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Plos One. - 14 : 11 (2019), p. e0224936. -
További szerzők:Nánási Péter Pál ifj. (1987-) (sejtbiológus) Imre László (1979-) (biológus) Dienes Beatrix (1972-) (élettanász, molekuláris biológus) Szöőr Árpád (1984-) (orvos) Türk-Mázló Anett (1989-) (molekuláris biológus) Kovács Attila (fizikus) Seidel, Ralf Vámosi György (1967-) (biofizikus) Szabó Gábor (1953-) (biofizikus)
Pályázati támogatás:GINOP-2.2.1-15-2017-00044
GINOP
K-128770
OTKA
COST-CA-1521
Egyéb
Stipendium Hungaricum
Egyéb
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
DOI
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2.

001-es BibID:BIBFORM085971
035-os BibID:(cikkazonosító)e0231223 (WOS)000536011400032 (Scopus)85083478527
Első szerző:Nánási Péter Pál ifj. (sejtbiológus)
Cím:Doxorubicin induces large-scale and differential H2A and H2B redistribution in live cells / Nánási Péter, Imre László, Firouzi Niaki Erfaneh, Bosire Rosevalentine, Mocsár Gábor, Türk-Mázló Anett, Ausio Juan, Szabó Gábor
Dátum:2020
ISSN:1932-6203
Megjegyzések:We observed prominent effects of doxorubicin (Dox), an anthracycline widely used in anticancer therapy, on the aggregation and intracellular distribution of both partners of the H2AH2B dimer, with marked differences between the two histones. Histone aggregation, assessed by Laser Scanning Cytometry via the retention of the aggregates in isolated nuclei, was observed in the case of H2A. The dominant effect of the anthracycline on H2B was its massive accumulation in the cytoplasm of the Jurkat leukemia cells concomitant with its disappearance from the nuclei, detected by confocal microscopy and mass spectrometry. A similar effect of the anthracycline was observed in primary human lymphoid cells, and also in monocyte-derived dendritic cells that harbor an unusually high amount of H2B in their cytoplasm even in the absence of Dox treatment. The nucleo-cytoplasmic translocation of H2B was not affected by inhibitors of major biochemical pathways or the nuclear export inhibitor leptomycin B, but it was completely diminished by PYR-41, an inhibitor with pleiotropic effects on protein degradation pathways. Dox and PYR-41 acted synergistically according to isobologram analyses of cytotoxicity. These large-scale effects were detected already at Dox concentrations that may be reached in the typical clinical settings, therefore they can contribute both to the anti-cancer mechanism and to the side-effects of this anthracycline.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Plos One. - 15 : 4 (2020), p. 1-23. -
További szerzők:Imre László (1979-) (biológus) Firouzi Niaki, Erfaneh Bosire, Rosevalentine (1988-) (biotechnológus) Mocsár Gábor (1981-) (biofizikus) Türk-Mázló Anett (1989-) (molekuláris biológus) Ausio, Juan Szabó Gábor (1953-) (biofizikus)
Pályázati támogatás:GINOP-2.3.3-15-2016-00020
GINOP
GINOP-2.3.2-15-2016-00044
GINOP
K128770
OTKA
COST EuroCellNet CA15214
Egyéb
Richter Gedeon Talentum Foundation
Egyéb
Stipendium Hungaricum, Tempus Public foundation
Egyéb
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:
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