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001-es BibID:	BIBFORM065042
035-os BibID:	(WoS)000365931100006 (Scopus)84948451094
Első szerző:	Guttman András (vegyészmérnök)
Cím:	Effect of Separation Temperature on Structure Specific Glycan Migration in Capillary Electrophoresis / Andras Guttman, Marta Kerekgyarto, Gabor Jarvas
Dátum:	2015
ISSN:	0003-2700
Megjegyzések:	Temperature dependent differential migration shifts were studied in capillary electrophoresis between linear (maltooligosaccharides) and branched (sialylated, neutral and core fucosylated biantennary IgG glycans) carbohydrates. Background electrolytes without as well as with low and high molecular weight additives (ethylene glycol, linear polyacrylamide and poly(ethylene oxide)) were investigated for this phenomena in the temperature range of 20-50 ?C. Glucose unit (GU) value shifts were observed with increasing temperature for the all IgG glycans both in additive-free and additive-containing background electrolytes, emphasizing the importance of tight temperature control during glycosylation analysis by capillary electrophoresis. The activation energy concept was applied to understand the structure specific electrophoretic migration of the different sugar molecules. Activation energy values were derived from the slopes of the Arrhenius plots of logarithmic mobility vs reciprocal absolute temperature and compared for the linear and branched sugars as well as for the various background electrolyte additives.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk capillary electrophoresis glycan structure mobility temperature activation energy
Megjelenés:	Analytical Chemistry. - 87 : 23 (2015), p. 11630-11634. -
További szerzők:	Kerékgyártó Márta (1987-) (molekuláris biológus) Járvás Gábor (1982-) (vegyészmérnök)
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM050948
Első szerző:	Szekrényes Ákos (vegyészmérnök)
Cím:	High-throughput analysis of therapeutic and diagnostic monoclonal antibodies by multicapillary SDS gel electrophoresis in conjunction with covalent fluorescent labeling / Ákos Szekrényes, Udo Roth, Márta Kerékgyártó, Andrea Székely, István Kurucz, Karen Kowalewski, András Guttman
Dátum:	2012
ISSN:	1618-2642
Megjegyzések:	Capillary gel electrophoresis (CGE) in the presence of sodium dodecyl sulfate (SDS) is a well-established and widely used protein analysis technique in the biotechnology industry, and increasingly becoming the method of choice that meets the requirements of the standards of International Conference of Harmonization (ICH). Automated single channel capillary electrophoresis systems are usually equipped with UV absorbance and/or laser-induced fluorescent (LIF) detection options offering general applicability and high detection sensitivity, respectively; however, with limited throughput. This shortcoming is addressed by the use of multicapillary gel electrophoresis (mCGE) systems with LED-induced fluorescent detection (LED-IF), also featuring automation and excellent detection sensitivity, thus widely applicable to rapid and large-scale analysis of biotherapeutics, especially monoclonal antibodies (mAb). The methodology we report in this paper is readily applicable for rapid purity assessment and subunit characterization of IgG molecules including detection of non-glycosylated heavy chains (NGHC) and separation of possible subunit variations such as truncated light chains (Pre-LC) or alternative splice variants. Covalent fluorophore derivatization and the mCGE analysis of the labeled IgG samples with multi-capillary gel electrophoresis are thoroughly described. Reducing and non-reducing conditions were both applied with and without peptide N-glycosidase F mediated deglycosylation.
Tárgyszavak:	Természettudományok Kémiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban Biotherapeutics Quality analysis Multicapillary gel electrophoresis Antibody purity Fluorescent labeling
Megjelenés:	Analytical and Bioanalytical Chemistry. - 404 : 5 (2012), p. 1485-1494. -
További szerzők:	Roth, Udo Kerékgyártó Márta (1987-) (molekuláris biológus) Székely Andrea Kurucz István Kowalewski, Karen Guttman András (1954-) (vegyészmérnök)
Pályázati támogatás:	K-81839 OTKA TECH-09-A1-2009-0113 Egyéb
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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