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001-es BibID:	BIBFORM105565
035-os BibID:	(cikkazonosító)483 (WOS)000564555500001 (Scopus)85088978014
Első szerző:	Kolics Éva
Cím:	An Alternative, High Throughput Method to Identify Csd Alleles of the Honey Bee / Éva Kolics, Tamás Parrag, Ferenc Házi, Kinga Szepesi, Botond Heltai, Kinga Mátyás, Barbara Kutasy, Eszter Virág, János Taller, László Orbán, Balázs Kolics
Dátum:	2020
ISSN:	2075-4450
Megjegyzések:	Applying instrumental insemination in closely related honey bee colonies often leads to frequent lethality of offspring causing colony collapse. This is due to the peculiarities of honey bee reproductive biology, where thecomplementary sex determination(csd) gene drives sex determination within a haplodiploid system. Diploid drones containing homozygous genotypes are lethal. Tracking ofcsdalleles using molecular markers prevents this unwanted event in closed breeding programs. Our approach described here is based on high throughput sequencing (HTS) that provides more data than traditional molecular techniques and is capable of analysing sources containing multiple alleles, including diploid individuals as the bee queen. The approach combines HTS technique and clipping wings as a minimally invasive method to detect the complementary sex determiner (csd) alleles directly from honey bee queens. Furthermore, it might also be suitable for screening alleles of honey harvested from hives of a closed breeding facility. Data on alleles of thecsdgene from different honey bee subspecies are provided. It might contribute to future databases that could potentially be used to track the origin of honey. With the help of trackingcsdalleles, more focused crossings will be possible, which could in turn accelerate honey bee breeding programmes targeting increase tolerance against varroosis as well.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk Apis mellifera hypervariable region alleles of csd gene molecular markers queen breeding
Megjelenés:	Insects. - 11 : 8 (2020), p. 1-12. -
További szerzők:	Parrag Tamás Házi Ferenc Szepesi Kinga Heltai Botond Mátyás Kinga Kutasy Barbara Virág Eszter (1982-) (biológus) Taller János Orbán László Kolics Balázs
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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