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001-es BibID:	BIBFORM105558
035-os BibID:	(cikkazonosító)118 (WOS)000633582500001 (Scopus)85103070905
Első szerző:	Kutasy Barbara
Cím:	Detection of Target-Site Herbicide Resistance in the Common Ragweed: Nucleotide Polymorphism Genotyping by Targeted Amplicon Sequencing / Barbara Kutasy, Zoltán Farkas, Balázs Kolics, Kincső Decsi, Géza Hegedűs, Judit Kovács, János Taller, Zoltán Tóth, Nikoletta Kálmán, Gabriella Kazinczi, Eszter Virág
Dátum:	2021
ISSN:	1424-2818
Megjegyzések:	Background: The spread of herbicide-resistance Ambrosia artemisiifolia threatens not only the production of agricultural crops, but also the composition of weed communities. The reduction of their spread would positively affect the biodiversity and beneficial weed communities in the arable habitats. Detection of resistant populations would help to reduce herbicide exposure which may contribute to the development of sustainable agroecosystems. Methods: This study focuses on the application of target-site resistance (TSR) diagnostic of A. artemisiifolia caused by different herbicides. We used targeted amplicon sequencing (TAS) on Illumina Miseq platform to detect amino acid changes in herbicide target enzymes of resistant and wild-type plants. Results: 16 mutation points of four enzymes targeted by four herbicide groups, such as Photosystem II (PSII), Acetohydroxyacid synthase (AHAS), 5-enolpyruvylshikimate 3-phosphate synthase (EPSPS) and protoporphyrinogen IX oxidase (PPO) inhibitors have been identified in common ragweed populations, so far. All the 16 mutation points were analyzed and identified. Out of these, two mutations were detected in resistant biotypes. Conclusions: The applied next-generation sequencing-targeted amplicon sequencing (NGS-TAS) method on A. artemisiifolia resistant and wild-type populations enable TSR detection of large sample numbers in a single reaction. The NGS-TAS provides information about the evolved herbicide resistance that supports the integrated weed control through the reduction of herbicide exposure which may preserve ecological properties in agroecosystems.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk target-site herbicide resistance ahas als psbA epsps targeted amplicon sequencing Ambrosia artemisiifolia
Megjelenés:	Diversity. - 13 : 3 (2021), p. 1-16. -
További szerzők:	Farkas Zoltán Kolics Balázs Decsi Kincső Hegedűs Géza Kovács Judit Taller János Tóth Zoltán Kálmán Nikoletta Kazinczi Gabriella Virág Eszter (1982-) (biológus)
Pályázati támogatás:	GINOP-2.3.4-15-2016-00005 GINOP EFOP3.6.3-VEKOP-16-2017-00008 Egyéb
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001-es BibID:	BIBFORM105557
035-os BibID:	(cikkazonosító)6648 (WOS)000666353700001 (Scopus)85108655353
Első szerző:	Kutasy Barbara
Cím:	Pro197Thr Substitution in Ahas Gene Causing Resistance to Pyroxsulam Herbicide in Rigid Ryegrass (Lolium Rigidum Gaud.) / Barbara Kutasy, Zsolt Takács, Judit Kovács, Verëlindë Bogaj, Syafiq A. Razak, Géza Hegedűs, Kincső Decsi, Kinga Székvári, Eszter Virág
Dátum:	2021
ISSN:	2071-1050
Megjegyzések:	: Lolium rigidum Gaud. is a cross-pollinated species characterized by high genetic diversity and it was detected as one of the most herbicide resistance-prone weeds, globally. Acetohydroxyacid synthase (AHAS) resistant populations cause significant problems in cereal production; therefore, monitoring the development of AHAS resistance is widely recommended. Using next-generation sequencing (NGS), a de novo transcriptome sequencing dataset was presented to identify the complete open reading frame (ORF) of AHAS enzyme in L. rigidum and design markers to amplify fragments consisting of all of the eight resistance-conferring amino acid mutation sites. Pro197Thr, Pro197Ala, Pro197Ser, Pro197Gln, and Trp574Leu amino acid substitutions have been observed in samples. Although the Pro197Thr amino acid substitution was already described in SU and IMI resistant populations, this is the first report to reveal that the Pro197Thr in AHAS enzyme confers a high level of resistance (ED50 3.569) to pyroxsulam herbicide (Triazolopyrimidine).
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk target-site resistance ahas als triazolopyrimidine herbicide lolium rigidum
Megjelenés:	Sustainability. - 13 : 12 (2021), p. 1-10. -
További szerzők:	Takács Zsolt Kovács Judit Bogaj, Verëlindë Razak, Syafiq A. Hegedűs Géza Decsi Kincső Székvári Kinga Virág Eszter (1982-) (biológus)
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