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001-es BibID:	BIBFORM105553
035-os BibID:	(Scopus)85132303780 (WoS)000816147800001
Első szerző:	Mutuku, Christopher
Cím:	Characterization of Beta-Lactamases and Multidrug Resistance Mechanisms in Enterobacterales from Hospital Effluents and Wastewater Treatment Plant / Christopher Mutuku, Szilvia Melegh, Krisztina Kovacs, Peter Urban, Eszter Virág, Reka Heninger, Robert Herczeg, Ágnes Sonnevend, Attila Gyenesei, Csaba Fekete, Zoltan Gazdag
Dátum:	2022
ISSN:	2079-6382
Megjegyzések:	Antimicrobials in wastewater promote the emergence of antibiotic resistance, facilitated by selective pressure and transfer of resistant genes. Enteric bacteria belonging to Escherichia coli, Klebsiella pneumoniae, Klebsiella oxytoca, Enterobacter cloacae, and Citrobacter species (n = 126) from hospital effluents and proximate wastewater treatment plant were assayed for susceptibility to four antimicrobial classes. The beta-lactamase encoding genes harbored in plasmids were genotyped and the plasmids were sequenced. A multidrug resistance phenotype was found in 72% (n = 58) of E. coli isolates, 70% (n = 43) of Klebsiella species isolates, and 40% (n = 25) of Enterobacter and Citrobacter species. Moreover, 86% (n = 50) of E. coli, 77% (n = 33) of Klebsiella species, and 25% (n = 4) of Citrobacter species isolates phenotypically expressed extended spectrum beta-lactamase. Regarding ESBL genes, bla(CTX-M-27) and bla(TEM-1) were found in E. coli, while Klebsiella species harbored bla(CTX-M-15), bla(CTX-M-30), or bla(SHV-12). Genes coding for aminoglycoside modifying enzymes, adenylyltransferases (aadA1, aadA5), phosphotransferases (aph(6)-1d, aph(3 '')-Ib), acetyltransferases (aac(3)-IIa), (aac(6)-Ib), sulfonamide/trimethoprim resistant dihydropteroate synthase (sul), dihydrofolate reductase (dfrA), and quinolone resistance protein (qnrB1) were also identified. Monitoring wastewater from human sources for acquired resistance in clinically important bacteria may provide a cheaper alternative in regions facing challenges that limit clinical surveillance.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk hospital effluents wastewater treatment plant Enterobacterales Béta-lactamases multiresistance
Megjelenés:	Antibiotics-Basel. - 11 : 6 (2022), p. 776-. -
További szerzők:	Melegh Szilvia Kovács Krisztina Urbán Péter Virág Eszter (1982-) (biológus) Heninger Réka Herczeg Róbert Sonnevend Ágnes Gyenesei Attila Fekete Csaba Gazdag Zoltán
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001-es BibID:	BIBFORM105350
035-os BibID:	(cikkazonosító)108736
Első szerző:	Mutuku, Christopher
Cím:	Plasmid sequence dataset of multidrug-resistant Enterobacterales isolated from hospital effluents and wastewater treatment plant / Christopher Mutuku, Barbara Kutasy, Peter Urban, Szilvia Melegh, Robert Herczeg, Zoltan Gazdag, Eszter Virág
Dátum:	2022
ISSN:	2352-3409
Megjegyzések:	We present plasmid sequences of 21 multidrug resistant isolates of Enterobacterales belonging to Escherichia coli (n=10), Klebsiella pneumoniae (n=9), Klebsiella oxytoca (n=1), and Citrobacter freundii (n=1). The isolates originated from effluent collected from hospital sewer pipes and from a wastewater treatment plant (WWTP) in a southwestern Hungarian city. Isolation was carried out using eosin methylene blue agar supplemented with ceftriaxone and the isolates were identified with MALDI-TOF MS. Screening for multidrug resistance was conducted by determining susceptibility to four chemical classes namely, beta-lactams, aminoglycoside, fluoroquinolone, and sulfonamide. Plasmid DNA was isolated by alkaline lysis method using the Monarch plasmid DNA miniprep kit from freshly grown pure colonies. Molecular typing and Illumina sequencing of plasmid DNA of multiresistant strains were performed. After the assembly of contigs, genes localized on plasmid sequences were determined and functionally annotated. These reconstructed plasmid sequences supplemented with gene functional annotations were deposited in the Mendeley data. Using these datasets different plasmid incompatibility groups were identified. These conjugative plasmids appear to play a key role in the transmission of multiple resistance genes in enteric bacteria via wastewater. The presented data may provide useful insight on the correlations between environmental antibiotic contamination and the development of bacterial resistance, which poses a serious public health threat.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk Hospital effluents Wastewater treatment plant Enterobacterales Beta-lactamases Resistance genes Plasmid antibiotic resistance
Megjelenés:	Data in Brief. - 45 (2022), p. 1-9. -
További szerzők:	Kutasy Barbara Urbán Péter Melegh Szilvia Herczeg Róbert Gazdag Zoltán Virág Eszter (1982-) (biológus)
Pályázati támogatás:	PTE Egyéb
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