CCL

Összesen 1 találat.
#/oldal:
Részletezés:
Rendezés:

1.

001-es BibID:BIBFORM105558
035-os BibID:(cikkazonosító)118 (WOS)000633582500001 (Scopus)85103070905
Első szerző:Kutasy Barbara
Cím:Detection of Target-Site Herbicide Resistance in the Common Ragweed: Nucleotide Polymorphism Genotyping by Targeted Amplicon Sequencing / Barbara Kutasy, Zoltán Farkas, Balázs Kolics, Kincső Decsi, Géza Hegedűs, Judit Kovács, János Taller, Zoltán Tóth, Nikoletta Kálmán, Gabriella Kazinczi, Eszter Virág
Dátum:2021
ISSN:1424-2818
Megjegyzések:Background: The spread of herbicide-resistance Ambrosia artemisiifolia threatens not only the production of agricultural crops, but also the composition of weed communities. The reduction of their spread would positively affect the biodiversity and beneficial weed communities in the arable habitats. Detection of resistant populations would help to reduce herbicide exposure which may contribute to the development of sustainable agroecosystems. Methods: This study focuses on the application of target-site resistance (TSR) diagnostic of A. artemisiifolia caused by different herbicides. We used targeted amplicon sequencing (TAS) on Illumina Miseq platform to detect amino acid changes in herbicide target enzymes of resistant and wild-type plants. Results: 16 mutation points of four enzymes targeted by four herbicide groups, such as Photosystem II (PSII), Acetohydroxyacid synthase (AHAS), 5-enolpyruvylshikimate 3-phosphate synthase (EPSPS) and protoporphyrinogen IX oxidase (PPO) inhibitors have been identified in common ragweed populations, so far. All the 16 mutation points were analyzed and identified. Out of these, two mutations were detected in resistant biotypes. Conclusions: The applied next-generation sequencing-targeted amplicon sequencing (NGS-TAS) method on A. artemisiifolia resistant and wild-type populations enable TSR detection of large sample numbers in a single reaction. The NGS-TAS provides information about the evolved herbicide resistance that supports the integrated weed control through the reduction of herbicide exposure which may preserve ecological properties in agroecosystems.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
target-site herbicide resistance
ahas
als
psbA
epsps
targeted amplicon sequencing
Ambrosia artemisiifolia
Megjelenés:Diversity. - 13 : 3 (2021), p. 1-16. -
További szerzők:Farkas Zoltán Kolics Balázs Decsi Kincső Hegedűs Géza Kovács Judit Taller János Tóth Zoltán Kálmán Nikoletta Kazinczi Gabriella Virág Eszter (1982-) (biológus)
Pályázati támogatás:GINOP-2.3.4-15-2016-00005
GINOP
EFOP3.6.3-VEKOP-16-2017-00008
Egyéb
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:
Rekordok letöltése1