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001-es BibID:	BIBFORM071904
Első szerző:	Kecskeméti Ádám (vegyész)
Cím:	Particle-based immobilized enzymatic reactors in microfluidic chips / Kecskemeti Adam, Gaspar Attila
Dátum:	2018
ISSN:	0039-9140
Megjegyzések:	The research and applications of immobilized enzyme reactors (IMERs) have become more and more widespread due to the numerous advantages like reusability, easy handling, prolonged lifetime, easy separation from pro- ducts and substrate specificity. The miniaturized form of these reactors (microchip IMERs) received outstanding attention due to their special features and advantages over the traditional, larger analytical systems. Large specific surface is essential for the efficient operation of the microreactors, thus these devices include one of the several types of porous solid supports, but in this work only the particle based microchip IMERs are reviewed. A very large variety of micro- or nanoparticles (beads) have been used in the microchip IMERs, however, in- corporating these particles into microchips is still a challenge, because the common procedures used for the preparation of chromatographic columns are not well applicable at the microscopic level. Many detection systems were applied with microchip IMERs using on-chip or off-chip arrangement. The combination of microchip IMERs with mass spectrometry is particularly popular, because in these systems high- throughput analysis can be achieved by which the proteomic studies can be largely accelerated. In most chip IMER-MS systems, the chips are used for sample pretreatment including analyte (protein) digestion, pre- concentration of analyte, removal of matrix materials. Additional applications of the IMERs - like the rapid protein digestion with proteolytic enzymes, the transformation of analytes to a more easily or more sensitively measurable form (detection signal amplification) and the design of microarrays/biosensors to analyze antigens based on specific interactions in immunoanalytical studies ? are also reviewed.
Tárgyszavak:	Természettudományok Kémiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban Microfluidic Enzyme reactor Particle Enzyme immobilization Protein digestion
Megjelenés:	Talanta. - 180 (2018), p. 211-228. -
További szerzők:	Gáspár Attila (1970-) (vegyész, kémikus)
Pályázati támogatás:	OTKA-111932 OTKA ÚNKP-17-3 Egyéb GINOP-2.3.3-15-2016-00004 GINOP GINOP-2.3.2-15-2016-00008 GINOP
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM068560
Első szerző:	Kecskeméti Ádám (vegyész)
Cím:	Preparation and characterization of a packed bead immobilized trypsin reactor integrated into a PDMS microfluidic chip for rapid protein digestion / Ádám Kecskeméti, Attila Gáspár
Dátum:	2017
ISSN:	0039-9140
Megjegyzések:	This paper demonstrates the design, efficiency and applicability of a simple, inexpensive and high samplethroughput microchip immobilized enzymatic reactor (IMER) for rapid protein digestion. The IMER containsconventional silica particles with covalently immobilized trypsin packed inside of a poly(dimethylsiloxane)(PDMS) microchip channel (10 mm?1 mm?35 ?m). The microchip consists of 9 different channels, enabling 9simultaneous protein digestions. Trypsin was covalently immobilized using carbodiimide activation, the idealtrypsin/silica particle ratio (i.e. measured mass ratio before the immobilization reaction) was determined. Theamount of immobilized trypsin was 10?15 ?g trypsin for 1 mg silica particle. Migration times of CZE peptidemaps showed good repeatability and reproducibility (RSD%=0.02?0.31%). The IMER maintained its activity for2 months, in this period it was used effectively for rapid proteolysis. Four proteins (myoglobin, lysozyme,hemoglobin and albumin) in a wide size range (15?70 kDa) were digested to demonstrate the applicability ofthe reactor. Their CZE peptide maps were compared to peptide maps obtained from standard in-solutiondigestion of the four proteins. The number of peptide peaks correlated well with the theoretically expectedpeptide number in both cases, the peak patterns of the electropherograms were similar, however, digestion withthe microchip IMER requires only < 10 s, while in-solution digestion takes 16 h. LC-MS/MS peptide mappingwas also carried out, the four proteins were identified with satisfying sequence coverages (29?50%), trypsinautolysis peptides were not detected. The protein content of human serum was digested with the IMER and within-solution digestion.
Tárgyszavak:	Természettudományok Kémiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban Trypsin immobilization Protein digestion Peptide mapping Microchip reactor
Megjelenés:	Talanta. - 166 (2017), p. 275-283. -
További szerzők:	Gáspár Attila (1970-) (vegyész, kémikus)
Pályázati támogatás:	OTKA-111932 OTKA NTP-NFTÖ-16-0038 Egyéb GINOP-2.3.2-15-2016-00008 GINOP GINOP-2.3.3-15-2016-00004 GINOP
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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