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1.
001-es BibID:
BIBFORM072747
Első szerző:
Morton, C. Oliver
Cím:
Determining the analytical specificity of PCR-based assays for the diagnosis of IA : what is Aspergillus? / C. Oliver Morton, P. Lewis White, Rosemary A. Barnes, Lena Klingspor, Manuel Cuenca-Estrella, Katrien Lagrou, Stéphane Bretagne, Willem Melchers, Carlo Mengoli, Angela M. Caliendo, Massimo Cogliati, Yvette Debets-Ossenkopp, Rebecca Gorton, Ferry Hagen, Catriona Halliday, Petr Hamal, Kathleen Harvey-Wood, Katia Jaton, Gemma Johnson, Sarah Kidd, Martina Lengerova, Cornelia Lass-Florl, Chris Linton, Laurence Millon, C. Orla Morrissey, Melinda Paholcsek, Alida Fe Talento, Markus Ruhnke, Birgit Willinger, J. Peter Donnelly, Juergen Loeffler, EAPCRI
Dátum:
2017
ISSN:
1369-3786
Megjegyzések:
A wide array of PCR tests has been developed to aid the diagnosis of invasive aspergillosis (IA), providing technical diversity but limiting standardisation and acceptance. Methodological recommendations for testing blood samples using PCR exist, based on achieving optimal assay sensitivity to help exclude IA. Conversely, when testing more invasive samples (BAL, biopsy, CSF) emphasis is placed on confirming disease, so analytical specificity is paramount. This multicenter study examined the analytical specificity of PCR methods for detecting IA by blind testing a panel of DNA extracted from a various fungal species to explore the range of Aspergillus species that could be detected, but also potential cross reactivity with other fungal species. Positivity rates were calculated and regression analysis was performed to determine any associations between technical specifications and performance. The accuracy of Aspergillus genus specific assays was 71.8%, significantly greater (P < .0001) than assays specific for individual Aspergillus species (47.2%). For genus specific assays the most often missed species were A. lentulus (25.0%), A. versicolor (24.1%), A. terreus (16.1%), A. flavus (15.2%), A. niger (13.4%), and A. fumigatus (6.2%). There was a significant positive association between accuracy and using an Aspergillus genus PCR assay targeting the rRNA genes (P = .0011). Conversely, there was a significant association between rRNA PCR targets and false positivity (P = .0032). To conclude current Aspergillus PCR assays are better suited for detecting A. fumigatus, with inferior detection of most other Aspergillus species. The use of an Aspergillus genus specific PCR assay targeting the rRNA genes is preferential.
Tárgyszavak:
Orvostudományok
Klinikai orvostudományok
idegen nyelvű folyóiratközlemény külföldi lapban
Aspergillus PCR
analytical specificity
cross reactivity
detection range
Megjelenés:
Medical Mycology 55 : 4 (2017), p. 402-413. -
További szerzők:
White, P. Lewis
Barnes, Rosemary A.
Klingspor, Lena
Cuenca-Estrella, Manuel
Lagrou, Katrien
Bretagne, Stéphane
Melchers, Willem
Mengoli, Carlo
Caliendo, Angela M.
Cogliati, Massimo
Debets-Ossenkopp, Yvette
Gorton, Rebecca
Hagen, Ferry
Halliday, Catriona
Hamal, Petr
Harvey-Wood, Kathleen
Jaton, Katia
Johnson, Gemma
Kidd, Sarah
Lengerova, Martina
Lass-Florl, Cornelia
Linton, Chris
Millon, Laurence
Morrissey, C. Orla
Paholcsek Melinda (1984-) (molekuláris biológus, genetikus)
Talento, Alida Fe
Ruhnke, Markus
Willinger, Birgit
Donnelly, J. Peter
Loeffler, Juergen
EAPCRI
Internet cím:
Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
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Saját polcon:
2.
001-es BibID:
BIBFORM053143
Első szerző:
Springer, Jan
Cím:
Multicenter comparison of serum and whole-blood specimens for detection of Aspergillus DNA in high-risk hematological patients / Jan Springer, C. O. Morton, Michael Perry, Werner J. Heinz, Melinda Paholcsek, Mona Alzheimer, T. R. Rogers, Rosemary A. Barnes, Hermann Einsele, Juergen Loeffler, P. Lewis White
Dátum:
2013
ISSN:
0095-1137
Megjegyzések:
Samples from patients at high risk for invasive aspergillosis (IA) were prospectively collected and analyzed for the presence of molecular markers of fungal infection. Serum specimens were screened for galactomannan and Aspergillus DNA, and whole-blood specimens were screened only for Aspergillus DNA. Fungal infections were categorized according to the European Organization for Research and Treatment of Cancer/Invasive Fungal Infections Cooperative Group, National Institute of Allergy and Infectious Diseases Mycoses Study Group (EORTC/MSG) criteria. Forty-seven cases (proven and probable IA) and 31 controls (no evidence of IA) were selected retrospectively for this case-control study, comprising 803 samples, in order to determine the performance of whole-blood PCR, serum PCR, and serum galactomannan testing. Although no single assay was able to detect every case of IA, a combination of different assays provided the best performance. There was no significant difference between the use of whole-blood and serum specimens for PCR-based diagnosis of IA, but there was a trend for whole blood to be more sensitive (85% versus 79%) and to yield an earlier positive result (36 days versus 15 days) than for serum. However, DNA extraction from serum specimens is easier and faster than that from whole-blood specimens, and it allows the same specimen to be used for both galactomannan and PCR assays. In conclusion, the appropriate sample type for DNA extraction should be determined by the local requirements and the technical platforms available at each individual center. A combination of biomarker tests offered the best diagnostic utility for detecting IA.
Tárgyszavak:
Természettudományok
Biológiai tudományok
idegen nyelvű folyóiratközlemény külföldi lapban
DIAGNOSING INVASIVE ASPERGILLOSIS
REAL-TIME PCR
FUNGAL-INFECTIONS
FUMIGATUS DNA
Megjelenés:
Journal of Clinical Microbiology. - 51 : 5 (2013), p. 1445-1450. -
További szerzők:
Morton, C. Oliver
Perry, Michael
Heinz, Werner J.
Paholcsek Melinda (1984-) (molekuláris biológus, genetikus)
Alzheimer, Mona
Rogers, T. R.
Barnes, Rosemary A.
Einsele, Hermann
Loeffler, Juergen
White, P. Lewis
Pályázati támogatás:
German Federal Ministry of Research and Education (BMBF)
Egyéb
Trans-European Cooperation ERA-NET PathoGenoMics
Egyéb
Internet cím:
Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:
Saját polcon:
3.
001-es BibID:
BIBFORM083399
Első szerző:
White, P. Lewis
Cím:
Comment on: T2Candida MR as a predictor of outcome in patients with suspected invasive candidiasis starting empirical antifungal treatment : a prospective pilot study / P. Lewis White, Rosemary A. Barnes, Rebecca Gorton, Mario Cruciani, Juergen Loeffler, Fungal PCR Initiative
Dátum:
2019
Megjegyzések:
We read with interest the article of Munoz ~ et al.1 describing the performance of the T2Candida MR assay in patients receiving empirical antifungal therapy for suspected invasive candidiasis (IC).1 The study was well designed and fully warranted in attempting to address the demands of managing patients at risk of fungal disease and provides much needed real-life data on the performance of the T2 platform. However, unlike the authors, we feel the performance of the T2 assay in this study was insufficient to discontinue antifungal therapy.
Tárgyszavak:
Orvostudományok
Klinikai orvostudományok
hozzászólás
folyóiratcikk
Megjelenés:
The Journal of Antimicrobial Chemotherapy. - 74 (2019), p. 532-533. -
További szerzők:
Barnes, Rosemary A.
Gorton, Rebecca
Cruciani, Mario
Loeffler, Juergen
Paholcsek Melinda (1984-) (molekuláris biológus, genetikus)
Fungal PCR Initiative
Internet cím:
Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:
Saját polcon:
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