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001-es BibID:	BIBFORM096053
Első szerző:	Papp László Attila (genetikus)
Cím:	Molecular and comparative genomic analyses reveal evolutionarily conserved and unique features of the Schizosaccharomyces japonicus mycelial growth and the underlying genomic changes / Papp László Attila, Ács-Szabó Lajos, Batta Gyula, Miklós Ida
Dátum:	2021
ISSN:	0172-8083
Megjegyzések:	Fungal pathogens, from phytopathogenic fungus to human pathogens, are able to alternate between the yeast-like form and filamentous forms. This morphological transition (dimorphism) is in close connection with their pathogenic lifestyles and with their responses to changing environmental conditions. The mechanisms governing these morphogenetic conversions are still not fully understood. Therefore, we studied the filamentous growth of the less-known, non-pathogenic dimorphic fission yeast, S. japonicus, which belongs to an ancient and early evolved branch of the Ascomycota. Its RNA sequencing revealed that several hundred genes were up- or down-regulated in the hyphae compared to the yeast-phase cells. These genes belonged to different GO categories, confirming that mycelial growth is a rather complex process. The genes of transport- and metabolic processes appeared especially in high numbers among them. High expression of genes involved in glycolysis and ethanol production was found in the hyphae, while other results pointed to the regulatory role of the protein kinase A (PKA) pathway. The homologues of 49 S. japonicus filament-associated genes were found by sequence alignments also in seven distantly related dimorphic and filamentous species. The comparative genomic analyses between S. japonicus and the closely related but non-dimorphic S. pombe shed some light on the differences in their genomes. All these data can contribute to a better understanding of hyphal growth and those genomic rearrangements that underlie it.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk Bioinformatic analysis Dimorphism Hyphae Mycelial growth Schizosaccharomyces japonicus Transcriptional profiling
Megjelenés:	Current Genetics. - 67 : 6 (2021), p. 953-968. -
További szerzők:	Ács-Szabó Lajos (1985-) (biológus) Batta Gyula (1979-) (biológus) Miklós Ida (1962-) (biológus)
Pályázati támogatás:	EFOP-3.6.1-16-2016-00022 EFOP 20428-3/2018 FIKP K10172 OTKA
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001-es BibID:	BIBFORM093840
Első szerző:	Papp László Attila (genetikus)
Cím:	A modified culture medium and hyphae isolation method can increase quality of the RNA extracted from mycelia of a dimorphic fungal species / Papp László Attila, Ács-Szabó Lajos, Póliska Szilárd, Miklós Ida
Dátum:	2021
ISSN:	0172-8083
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk
Megjelenés:	Current Genetics. - 67 : 5 (2021), p. 823-830. -
További szerzők:	Ács-Szabó Lajos (1985-) (biológus) Póliska Szilárd (1978-) (biológus) Miklós Ida (1962-) (biológus)
Pályázati támogatás:	OTKA-106172 OTKA EFOP-3.6.1-16-2016-00022 EFOP 20428-3/2018 FIKP
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001-es BibID:	BIBFORM081302
Első szerző:	Papp László Attila (genetikus)
Cím:	Expression pattern and phenotypic characterization of the mutant strain reveals target genes and processes regulated by pka1 in the dimorphic fission yeast Schizosaccharomyces japonicus / László Papp, Matthias Sipiczki, Ida Miklós
Dátum:	2017
ISSN:	0172-8083
Megjegyzések:	The cAMP cascade plays an important role in several biological processes. Thus, study of its molecular details can contribute to a better understanding of these processes, treatment of diseases, or even finding antifungal drug targets. To gain further information about the PKA pathway, and its evolutionarily conserved and species-specific features, the central regulator pka1 gene, which encodes the cAMP-dependent protein kinase catalytic subunit, was studied in the less known haplontic, dimorphic fission yeast Schizosaccharomyces japonicus. Namely, this species belongs to a highly divergent phylogenetic branch of fungi. Furthermore, S. japonicus had only a single copy pka1 gene in contrast to the budding yeasts. Therefore, the pka1 deleted mutant was created, whose RNA sequencing and phenotypic studies revealed that the Pka1 regulated at least 373 genes, among them further kinases, phosphatases and transcriptional regulators. It regulated elongation of hyphae, cell size, aging and stress response. Furthermore, half of the pka1 target genes seemed to be conserved in Schizosaccharomyces pombe and S. japonicus. However, there were oppositely regulated genes in the two closely related species. The target genes suggest that this single gene must be able to fulfill all the functions of TPK1-3 of Saccharomyces cerevisiae. Thus, our results shed light on certain similarities and differences of the PKA pathway of S. japonicus compared to the budding yeasts and confirmed the multifunctionality of the pka1 gene, but further experiments are needed to prove its involvement in the metabolic processes and transport.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk pka1 cAMP cascade Protein kinase RNA sequencing Dimorphism Schizosaccharomyces japonicus
Megjelenés:	Current Genetics. - 63 : 3 (2017), p. 487-497. -
További szerzők:	Sipiczki Mátyás (1948-) (biológus) Miklós Ida (1962-) (biológus)
Pályázati támogatás:	OTKA K106172 OTKA SROP-4.2.2.B-15/1/KONV-2015-0001 Egyéb
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