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001-es BibID:	BIBFORM078528
035-os BibID:	(cikkazonosító)foy021
Első szerző:	Csoma Hajnalka (biológus, szőlész-borász)
Cím:	Application of different markers and data-analysis tools to the examination of biodiversity can lead to different results : a case study with Starmerella bacillaris (synonym Candida zemplinina) strains / Hajnalka Csoma, Lajos Ács-Szabó, László Attila Papp, Matthias Sipiczki
Dátum:	2018
ISSN:	1567-1364
Megjegyzések:	Starmerella bacillaris (Candida zemplinina) is a genetically heterogeneous species. In this work, the diversity of 41 strains of various origins is examined and compared by the analysis of the length polymorphism of nuclear microsatellites and the RFLP of mitochondrial genomes. The band patterns are analysed with UPGMA, neighbor joining, neighbor net, minimum spanning tree and non-metric MDS algorithms. The results and their comparison to previous analyses demonstrate that different markers and different clustering methods can result in very different groupings of the same strains. The observed differences between the topologies of the dendrograms also indicate that the positions of the strains do not necessarily reflect their real genetic relationships and origins. The possibilities that the differences might be partially due to different sensitivity of the markers to environmental factors (selection pressure) and partially to the different grouping criteria of the algorithms are also discussed.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk Candida zemplinina mtDNA microsatellite markers strain biodiversity RFLP-mtDNA
Megjelenés:	FEMS Yeast Research. - 18 : 5 (2018), p. 1-12. -
További szerzők:	Ács-Szabó Lajos (1985-) (biológus) Papp László Attila (1986-) (genetikus) Sipiczki Mátyás (1948-) (biológus)
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001-es BibID:	BIBFORM060997
035-os BibID:	(WOS)000345980600003 (Scopus)84913614417
Első szerző:	Papp László Attila (genetikus)
Cím:	Optimal conditions for mycelial growth of Schizosaccharomyces japonicus cells in liquid medium : it enables the molecular investigation of dimorphism / László Papp, Matthias Sipiczki, Imre J. Holb, Ida Miklós
Dátum:	2014
ISSN:	0749-503X
Megjegyzések:	The non-pathogenic dimorphic fission yeast, Schizosaccharomyces japonicus, could be a suitable model organism for investigation of the genetic background of mycelial growth, as it has a haploid chromosome set and its genome is sequenced. Since earlier results have suggested that its morphological transition required solid substrates, but molecular biological experiments would require hyphae production in a liquid medium, we wanted to find circumstances which would enable hyphae production in liquid media. Several external conditions were investigated, but the strongest inducer was fetal bovine serum (FBS). Its positive effect could be hampered by heat and was dependent on pH, temperature and concentration of the serum. Other protein-containing compounds, such as peptone and bovine serum albumin or amino acids, proved to be ineffective or weak. Generally, the uninduced and induced mycelial growth of Sz. japonicus could be improved by lower external pH and higher temperature.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk Schizosaccharomyces japonicus
Megjelenés:	Yeast. - 31 : 12 (2014), 475-482. -
További szerzők:	Sipiczki Mátyás (1948-) (biológus) Holb Imre (1973-) (agrármérnök) Miklós Ida (1962-) (biológus)
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