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001-es BibID:	BIBFORM065243
Első szerző:	Gonda Sándor (gyógyszerész)
Cím:	Myrosinase Compatible Simultaneous Determination of Glucosinolates and Allyl Isothiocyanate by Capillary Electrophoresis Micellar Electrokinetic Chromatography (CE-MEKC) / Sándor Gonda, Attila Kiss-Szikszai, Zsolt Szűcs, Nhat Minh Nguyen, Gábor Vasas
Dátum:	2016
ISSN:	0958-0344
Megjegyzések:	Introduction. The functional food Cruciferous vegetables contain glucosinolates which aredecomposed by the myrosinase enzyme upon tissue damage. The isothiocyanates are the most frequentdecomposition products. Because of their various bioactivities, these compounds and the myrosinase isof high interest to many scientific fields.Objective. Development of a capillary electrophoresis method capable of myrosinase-compatible, simultaneous quantification of glucosinolates and isothiocyanates.Methods. Capillary electrochromatography parameters were optimized, followed byoptimization of a myrosinase-compatible derivatization procedure for isothiocyanates. Vegetableextracts (Brussels sprouts, horseradish, radish and watercress) were tested for myrosinase activity,glucosinolate content and isothiocyanate conversion rate. Allyl isothiocyanate was quantified in somefood products.Results. The method allows quantification of sinigrin, gluonasturtiin and allyl isothiocyanateafter myrosinase compatible derivatization in-vial by mercaptoacetic acid. The chromatograhpicseparation takes 2.5 minutes (short end injection) or 15 minutes (long end injection). For the testedvegetables, measured myrosinase activity was between 0.960 ? 27.694 and 0.461 ? 26.322 ?mol min-1mg-1 protein, glucosinolate content was between 0 ? 2291.8 and 0 ? 248.5 ?g g-1 fresh weight forsinigrin and gluconastrutiin, respectively. The possible specificity of plants to different glucosinolateswas also shown. Allyl isothiocyanate release rate was different in different vegetables (73.13 to 102.13%). The method could also be used for quantification of allyl isothiocyanate from food products.Conclusions. The presented capillary electrophoresis method requires a minimal amount ofsample and contains only a few sample preparation steps, and can be used in several applications(glucosinolate determination, myrosinase activity measurement, isothiocyanate release estimation).
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban myrosinase assay in-vial derivatization Brassicaceae vegetables allyl isothiocyanate phenethyl isothiocyanate
Megjelenés:	Phytochemical Analysis 27 : 3-4 (2016), p. 191-198. -
További szerzők:	Kiss-Szikszai Attila (1975-) (vegyész) Szűcs Zsolt (1990-) (gyógyszerész) Nguyen, Minh Nhat (1984-) (gyógyszerész) Vasas Gábor (1975-) (biológus-vegyész)
Pályázati támogatás:	PD 112374 OTKA TÁMOP 4.2.2.A-11/1/KONV-2012-0043 TÁMOP
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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