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001-es BibID:	BIBFORM064340
035-os BibID:	(WoS)000382077800015 (Scopus)85013262896
Első szerző:	Csomós Krisztián (molekuláris biológus)
Cím:	Protein cross-linking by chlorinated polyamines and transglutamylation stabilizes neutrophil extracellular traps / Krisztián Csomós, Endre Kristóf, Bernadett Jakob, István Csomós, György Kovács, Omri Rotem, Judit Hodrea, Zsuzsa Bagoly, Laszlo Muszbek, Zoltán Balajthy, Éva Csősz, László Fésüs
Dátum:	2016
ISSN:	2041-4889
Megjegyzések:	Neutrophil extracellular trap (NET) ejected from activated dying neutrophils is a highly ordered structure of DNA and selected proteins capable to eliminate pathogenic microorganisms. Biochemical determinants of the non-randomly formed stable NETs have not been revealed so far. Studying the formation of human NETs we have observed that polyamines were incorporated into the NET. Inhibition of myeloperoxidase, which is essential for NET formation and can generate reactive chlorinated polyamines through hypochlorous acid, decreased polyamine incorporation. Addition of exogenous primary amines that similarly to polyamines inhibit reactions catalyzed by the protein cross-linker transglutaminases (TGases) has similar effect. Proteomic analysis of the highly reproducible pattern of NET components revealed cross-linking of NET proteins through chlorinated polyamines and epsilon(gamma-glutamyl) lysine as well as bis-gamma-glutamyl polyamine bonds catalyzed by the TGases detected in neutrophils. Competitive inhibition of protein cross-linking by monoamines disturbed the cross-linking pattern of NET proteins, which resulted in the loss of the ordered structure of the NET and significantly reduced capacity to trap bacteria. Our findings provide explanation of how NETs are formed in a reproducible and ordered manner to efficiently neutralize microorganisms at the first defense line of the innate immune system.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk NET
Megjelenés:	Cell Death & Disease. - 7 : 8 (2016), p. e2332. -
További szerzők:	Kristóf Endre (1987-) (általános orvos) Márkus Bernadett (1989-) (molekuláris biológus) Csomós István (1983-) (molekuláris biológus) Kovács György (1984-) (programtervező matematikus, fizikus) Rotem, Omri Hodrea Judit (1978-) (vegyész) Bagoly Zsuzsa (1978-) (orvos) Muszbek László (1942-) (haematológus, kutató orvos) Balajthy Zoltán (1957-) (biokémikus, sejtbiológus) Csősz Éva (1977-) (biokémikus, molekuláris biológus) Fésüs László (1947-) (orvos biokémikus)
Pályázati támogatás:	TÁMOP-4.2.2.A-11/1/KONV-2012-0023 TÁMOP TÁMOP-4.2.4.A/ 2-11/1-2012-0001 TÁMOP OTKA NK 105046 OTKA
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001-es BibID:	BIBFORM070868
Első szerző:	Márkus Bernadett (molekuláris biológus)
Cím:	Analysis of the protein crosslink-profile changes and the neutrophil extracellular trap patterns elicited by different stimuli / Bernadett Márkus, Endre Kristóf, István Csomós, László Fésüs, Éva Csősz
Dátum:	2017
Megjegyzések:	Mature neutrophils act as a first line of defence migrating to the site of infection in response to microbial invasion. They are able to attack pathogen directly in three different ways: phagocytosis, release of antimicrobial peptides and formation of neutrophil extracellular traps (NETs). NET is the result of a unique form of cell death in which neutrophils eject their mixture of nucleoplasm and cytoplasm into the extracellular space forming a web-like structure. Hereby the invaded pathogens are trapped, neutralized therefore their dissemination is inhibited. Several antimicrobial proteins and proteases (such as neutrophil elastase, myeloperoxidase, cathepsin G, azurocidin, lactoferrin) are essential elements of the NET contributing to direct and indirect antimicrobial activities. Based on MS/MS data of protein cross-links formed in the differently treated NETs we could observe protein cross-linking by chlorinated polyamines and transglutaminases and we could demonstrate that both myeloperoxidase and TG1 are required for the stabilization of NET (Csomos et al. Cell Death Dis. 2016). Various bacteria, fungi and viruses can induce NET formation therefore we aimed to investigate the changes in the protein crosslink-profile and in the complex web-like structure during NET formation elicited by different pathogens.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok előadáskivonat
Megjelenés:	Transglutaminases in Medicine. - p. 83.
További szerzők:	Kristóf Endre (1987-) (általános orvos) Csomós István (1983-) (molekuláris biológus) Fésüs László (1947-) (orvos biokémikus) Csősz Éva (1977-) (biokémikus, molekuláris biológus)
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM116662
035-os BibID:	(Scopus)85171172955 (WoS)001023372700001 (cikkazonosító)1249909
Első szerző:	Vámos Attila (gyógyszer-biotechnológus)
Cím:	Corrigendum : Human abdominal subcutaneous-derived active beige adipocytes carrying FTO rs1421085 obesity-risk alleles exert lower thermogenic capacity / Vámos Attila, Arianti Rini, Vinnai Boglárka Ágnes, Alrifai Rahaf, Shaw Abhirup, Póliska Szilárd, Guba Andrea, Csősz Éva, Csomós István, Mocsár Gábor, Lányi Cecilia, Balajthy Zoltán, Fésüs László, Kristóf Endre
Dátum:	2023
ISSN:	2296-634X
Megjegyzések:	In the published article, there was an error. In the published article, the Reference "Bjune et al., 2005" was cited with an incorrect year of publication. The correct year of publication is 2019. In the published article "Bjune, J. I., Haugen, C., Gudbrandsen, O., Nordb?, O. P., Nielsen, H. J., V?age, V., et al. (2019). IRX5 regulates adipocyte amyloid precursor protein and mitochondrial respiration in obesity. Int J Obes (Lond)., 43(11), 2151?2162. https://doi.org/10.1038/s41366-018-0275-y" was not referenced in the article. The reference has now been inserted into the article. A correction has been made to the Introduction. This sentence previously stated: "In addition, IRX5 silencing increased the mitochondrial respiration in isolated mouse adipocytes (Bjune et al., 2005)." The corrected sentence appears below: "In addition, IRX5 silencing increased the mitochondrial respiration in isolated mouse adipocytes (Bjune et al., 2019)." The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated. Copyright ? 2023 Vámos, Arianti, Vinnai, Alrifai, Shaw, Póliska, Guba, Csősz, Csomós, Mocsár, Lányi, Balajthy, Fésüs and Kristóf.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok hozzászólás folyóiratcikk adipocytes beige FTO rs1421085 obesity SLC7A10 thermogenesis UCP 1
Megjelenés:	Frontiers in Cell and Developmental Biology. - 11 (2023), p. 1-2. -
További szerzők:	Arianti, Rini (1991-) (biokémikus) Vinnai Boglárka Ágnes (1996-) (molekuláris biológus) Alrifai, Rahaf Shaw, Abhirup (1992-) Póliska Szilárd (1978-) (biológus) Guba Andrea (1975-) (Okleveles vegyész) Csősz Éva (1977-) (biokémikus, molekuláris biológus) Csomós István (1983-) (molekuláris biológus) Mocsár Gábor (1981-) (biofizikus) Lányi Cecília Balajthy Zoltán (1957-) (biokémikus, sejtbiológus) Fésüs László (1947-) (orvos biokémikus) Kristóf Endre (1987-) (általános orvos)
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001-es BibID:	BIBFORM112108
035-os BibID:	(cikkazonosító)1155673 (scopus)85164495947 (wos)001023372700001
Első szerző:	Vámos Attila (gyógyszer-biotechnológus)
Cím:	Human Abdominal Subcutaneous-Derived Active Beige Adipocytes Carrying FTO rs1421085 Obesity-Risk Alleles Exert Lower Thermogenic Capacity / Vámos Attila, Arianti Rini, Vinnai Boglárka Ágnes, Alrifai Rahaf, Shaw Abhirup, Póliska Szilárd, Guba Andrea, Csősz Éva, Csomós István, Mocsár Gábor, Lányi Cecília, Balajthy Zoltán, Fésüs László, Kristóf Endre
Dátum:	2023
ISSN:	2296-634X
Megjegyzések:	White adipocytes store lipids, have a large lipid droplet and few mitochondria. Brown and beige adipocytes, which produce heat, are characterized by high expression of uncoupling protein (UCP) 1, multilocular lipid droplets, and large amounts of mitochondria. The rs1421085 T-to-C single-nucleotide polymorphism (SNP) of the human FTO gene interrupts a conserved motif for ARID5B repressor, resulting in adipocyte type shift from beige to white. We obtained abdominal subcutaneous adipose tissue from donors carrying FTO rs1421085 TT (risk-free) or CC (obesity-risk) genotypes, isolated and differentiated their preadipocytes into beige adipocytes (driven by the PPAR? agonist rosiglitazone for 14 days), and activated them with dibutyryl-cAMP for 4 hours. Then, either the same culture conditions were applied for additional 14 days (active beige adipocytes) or it was replaced by a white differentiation medium (inactive beige adipocytes). White adipocytes were differentiated by their medium for 28 days. RNA-sequencing was performed to investigate the gene expression pattern of adipocytes carrying different FTO alleles and found that active beige adipocytes had higher brown adipocyte content and browning capacity compared to white or inactive beige ones when the cells were obtained from risk-free TT but not from obesity-risk CC genotype carriers. Active beige adipocytes carrying FTO CC had lower thermogenic gene (e.g., UCP1, PM20D1, CIDEA) expression and thermogenesis measured by proton leak respiration as compared to TT carriers. In addition, active beige adipocytes with CC alleles exerted lower expression of ASC-1 neutral amino acid transporter (encoded by SLC7A10) and less consumption of Ala, Ser, Cys, and Gly as compared to risk-free carriers. We did not observe any influence of the FTO rs1421085 SNP on white and inactive beige adipocytes highlighting its exclusive and critical effect when adipocytes were activated for thermogenesis.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk adipocytes beige obesity FTO rs1421085 thermogenesis UCP1 SLC7A10
Megjelenés:	Frontiers in Cell and Developmental Biology. - 11 (2023), p. 1-18. -
További szerzők:	Arianti, Rini (1991-) (biokémikus) Vinnai Boglárka Ágnes (1996-) (molekuláris biológus) Alrifai, Rahaf Shaw, Abhirup (1992-) Póliska Szilárd (1978-) (biológus) Guba Andrea (1975-) (Okleveles vegyész) Csősz Éva (1977-) (biokémikus, molekuláris biológus) Csomós István (1983-) (molekuláris biológus) Mocsár Gábor (1981-) (biofizikus) Lányi Cecília Balajthy Zoltán (1957-) (biokémikus, sejtbiológus) Fésüs László (1947-) (orvos biokémikus) Kristóf Endre (1987-) (általános orvos)
Pályázati támogatás:	FK131424 OTKA K129139 OTKA ÚNKP-22-3-I-DE-30 Egyéb ÚNKP-22-3-II-DE-25 Egyéb
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