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001-es BibID:	BIBFORM112318
Első szerző:	Khangembam, Rojesh
Cím:	Recovery and confirmation of Haemonchus contortus from abomasal contents of roe deer (Capreolus capreolus) in Eastern-Hungary (Biharugra): A diagnostic case study / Rojesh Khangembam, Mariann Tóth, Gabriella Gulyas, Levente Czeglédi, Nóra Vass
Dátum:	2023
ISSN:	1587-1282
Megjegyzések:	Gastrointestinal parasites are ubiquitous. They occur both in wild and domesticated animals. Among such parasites of veterinary importance is the trichostrongyle worms, out of which the Haemonchus contortus species is regarded as the most pathogenic one in the sma ll ruminant industry. The occurrence of this parasite in the sheep flock is now very well documented and an established fact in Europe, a lthough the parasite was original of the warmer climatic region. Studies on the cross-transmission of H. contortus between the wild and domesticated animals are also on the rise although the question of the direction of transmission is still debated. This is an important area that needs to be addressed as it could potentially contribute indirectly to mitigating anthelmintic resistance. Hungary also has reported its share of the occurrence of the parasite, mainly in the sheep flock and a certain population of roe deer. The study presented here is the preliminary results of a diagnostic case study that confirms the presence of H. contortus in wild ruminant deer species that are close to the domesticated sheep population.
Tárgyszavak:	Agrártudományok Állatorvosi tudományok idegen nyelvű folyóiratközlemény hazai lapban folyóiratcikk Haemonchus contortus Roe deer Hungary PCR
Megjelenés:	Agrártudományi Közlemények = Acta agraria Debreceniensis. - 1 (2023), p. 59-62. -
További szerzők:	Tóth Mariann (1994-) (agrármérnök) Gulyás Gabriella (1985-) (agrár) Czeglédi Levente (1977-) (agrármérnök) Vass Nóra (1983-) (állatorvos)
Pályázati támogatás:	TKP2020-NKA-04 Egyéb
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat Szerző által megadott URL DOI
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001-es BibID:	BIBFORM101791
035-os BibID:	(cikkazonosító)82 (WoS)000730412700001 (Scopus)85122021532
Első szerző:	Khangembam, Rojesh
Cím:	Point of care colourimetric and lateral flow LAMP assay for the detection of Haemonchus contortus in ruminant faecal samples / Rojesh Khangembam, Mariann Tóth, Nóra Vass, Marián Várady, Levente Czeglédi, Róbert Farkas, Alistair Antonopoulos
Dátum:	2021
ISSN:	1776-1042
Megjegyzések:	In this study, we present an optimised colourimetric and a lateral flow LAMP assay for the detection of Haemonchus contortus in small ruminant faecal samples. Using a previously published LAMP primer set, we made use of commercially available colourimetric LAMP and lateral flow kits and combined this into an optimised diagnostic assay which was then tested on field faecal samples from Eastern and South-Eastern Hungary as well as a pure H. contortus egg faecal sample from Ko?sice, Slovakia. Both assays showed no conflicts in visual detection of the results. Additionally, we modified and tested several centrifuge-free DNA extraction methods and one bead-beating egg lysis DNA extraction method to develop a true point of care protocol, as the source of the starting DNA is the main ratelimiting step in farm-level molecular diagnosis. Out of the various methods trialed, promising results were obtained with the magnetic bead extraction method. Sample solutions from the Fill-FLOTAC technique were also utilised, which demonstrated that it could be efficiently adapted for field-level egg concentration to extract DNA. This proof of concept study showed that isothermal amplification technologies with a colourimetric detection or when combined with a lateral flow assay could be an important step for a true point of care molecular diagnostic assay for H. contortus.
Tárgyszavak:	Agrártudományok Állattenyésztési tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk LAMP assay Lateral flow Haemonchus contortus Point-of-Care Diagnosis Fill-FLOTAC
Megjelenés:	Parasite. - 28 (2021), p. 1-12. -
További szerzők:	Tóth Mariann (1994-) (agrármérnök) Vass Nóra (1983-) (állatorvos) Várady Marián Czeglédi Levente (1977-) (agrármérnök) Farkas Róbert Antonopoulos, Alistair
Pályázati támogatás:	EFOP-3.6.3-VEKOP-16-2017-00008 EFOP
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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