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001-es BibID:	BIBFORM112318
Első szerző:	Khangembam, Rojesh
Cím:	Recovery and confirmation of Haemonchus contortus from abomasal contents of roe deer (Capreolus capreolus) in Eastern-Hungary (Biharugra): A diagnostic case study / Rojesh Khangembam, Mariann Tóth, Gabriella Gulyas, Levente Czeglédi, Nóra Vass
Dátum:	2023
ISSN:	1587-1282
Megjegyzések:	Gastrointestinal parasites are ubiquitous. They occur both in wild and domesticated animals. Among such parasites of veterinary importance is the trichostrongyle worms, out of which the Haemonchus contortus species is regarded as the most pathogenic one in the sma ll ruminant industry. The occurrence of this parasite in the sheep flock is now very well documented and an established fact in Europe, a lthough the parasite was original of the warmer climatic region. Studies on the cross-transmission of H. contortus between the wild and domesticated animals are also on the rise although the question of the direction of transmission is still debated. This is an important area that needs to be addressed as it could potentially contribute indirectly to mitigating anthelmintic resistance. Hungary also has reported its share of the occurrence of the parasite, mainly in the sheep flock and a certain population of roe deer. The study presented here is the preliminary results of a diagnostic case study that confirms the presence of H. contortus in wild ruminant deer species that are close to the domesticated sheep population.
Tárgyszavak:	Agrártudományok Állatorvosi tudományok idegen nyelvű folyóiratközlemény hazai lapban folyóiratcikk Haemonchus contortus Roe deer Hungary PCR
Megjelenés:	Agrártudományi Közlemények = Acta agraria Debreceniensis. - 1 (2023), p. 59-62. -
További szerzők:	Tóth Mariann (1994-) (agrármérnök) Gulyás Gabriella (1985-) (agrár) Czeglédi Levente (1977-) (agrármérnök) Vass Nóra (1983-) (állatorvos)
Pályázati támogatás:	TKP2020-NKA-04 Egyéb
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat Szerző által megadott URL DOI
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001-es BibID:	BIBFORM108918
Első szerző:	Khangembam, Rojesh
Cím:	Point of care colourimetric and Lateral Flow-LAMP Assay for the detection of Haemonchus contortus from ruminant faecal samples. / Rojesh Khangembam, Mariann Tóth, Nóra Vass, Marián Várady, Róbert Farkas, Alistair Antonopoulos
Dátum:	2021
Tárgyszavak:	Agrártudományok Állatorvosi tudományok előadáskivonat könyvrészlet
Megjelenés:	28th International Conference of the World Association for the Advancement of Veterinary Parasitology WAAVP 2021 : Book of Abstracts / ed. Grace Mulcahy. - p. 178-179.
További szerzők:	Tóth Mariann (1994-) (agrármérnök) Vass Nóra (1983-) (állatorvos) Várady Marián Farkas Róbert Antonopoulos, Alistair
Internet cím:	Szerző által megadott URL Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM101791
035-os BibID:	(cikkazonosító)82 (WoS)000730412700001 (Scopus)85122021532
Első szerző:	Khangembam, Rojesh
Cím:	Point of care colourimetric and lateral flow LAMP assay for the detection of Haemonchus contortus in ruminant faecal samples / Rojesh Khangembam, Mariann Tóth, Nóra Vass, Marián Várady, Levente Czeglédi, Róbert Farkas, Alistair Antonopoulos
Dátum:	2021
ISSN:	1776-1042
Megjegyzések:	In this study, we present an optimised colourimetric and a lateral flow LAMP assay for the detection of Haemonchus contortus in small ruminant faecal samples. Using a previously published LAMP primer set, we made use of commercially available colourimetric LAMP and lateral flow kits and combined this into an optimised diagnostic assay which was then tested on field faecal samples from Eastern and South-Eastern Hungary as well as a pure H. contortus egg faecal sample from Ko?sice, Slovakia. Both assays showed no conflicts in visual detection of the results. Additionally, we modified and tested several centrifuge-free DNA extraction methods and one bead-beating egg lysis DNA extraction method to develop a true point of care protocol, as the source of the starting DNA is the main ratelimiting step in farm-level molecular diagnosis. Out of the various methods trialed, promising results were obtained with the magnetic bead extraction method. Sample solutions from the Fill-FLOTAC technique were also utilised, which demonstrated that it could be efficiently adapted for field-level egg concentration to extract DNA. This proof of concept study showed that isothermal amplification technologies with a colourimetric detection or when combined with a lateral flow assay could be an important step for a true point of care molecular diagnostic assay for H. contortus.
Tárgyszavak:	Agrártudományok Állattenyésztési tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk LAMP assay Lateral flow Haemonchus contortus Point-of-Care Diagnosis Fill-FLOTAC
Megjelenés:	Parasite. - 28 (2021), p. 1-12. -
További szerzők:	Tóth Mariann (1994-) (agrármérnök) Vass Nóra (1983-) (állatorvos) Várady Marián Czeglédi Levente (1977-) (agrármérnök) Farkas Róbert Antonopoulos, Alistair
Pályázati támogatás:	EFOP-3.6.3-VEKOP-16-2017-00008 EFOP
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM108670
Első szerző:	Tóth Mariann (agrármérnök)
Cím:	A case report: sheep endoparasitism dynamics under semi-dry continental climate of Karcag, Hungary / Mariann Tóth, Rojesh Khangembam, Róbert Farkas, János Oláh, Nóra Vass, István Monori
Dátum:	2019
ISSN:	1681-0015 2313-2191
Megjegyzések:	The main aim of the study was to have a preliminary assessment of the endoparasites which are infecting sheep under semi-dry continental climate in Karcag, Hungary. Two groups of Hungarian Merino sheep were assigned as treated (N=40) and untreated (N=20) after selecting the animals randomly. Only the Treated group was drenched two times with commercially available deworming drugs. Faecal samples were collected individually from both the groups to perform faecal egg count; body condition score and FAMACHA scores were also taken to assess body and anaemia conditions respectively. The temperature and humidity conditions were also obtained to check the optimum environmental condition that could influence the worm burden. The case study was done at the University of Debrecen Experimental Animal Farm, Karcag. Parasitic nematodes, namely, trichostrongylid/trichostrongyles nematodes, Protostrongylus sp. and Strongylus sp. were found to be more predominant species affecting the sheep and up to some degree with the tapeworm Moniezia sp. and the coccidian, Eimeria sp. During the study period, there was no clinically significant anaemic condition and the animals were found to have fairly good body condition. Yet, the infection intensity, mainly of the trichostrongyles, was significant even after the second drenching. This may be due to the optimum environmental condition coinciding with the grazing period which increased the parasitic loads in the fields. Another possibility is the presence of resistant worm population as there is no proper assessment of the effectiveness and/or resistance of the drenching drugs commonly used in Hungary. The study is only a preliminary report of endoparasitism of sheep under a particular Hungarian climatic condition. It is obvious that parasites, mainly of the nematodes, do occur and may be an increasing concern with time. Keeping this in mind, there is a need for a wider prevalence study and if possible, anthelmentic resistance studies of the commonly used drugs to check their efficacies.
Tárgyszavak:	Agrártudományok Állattenyésztési tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk Sheep endoparasite nematodes semi-dry environment
Megjelenés:	The Animal Biology. - 21 : 2 (2019), p. 66-69. -
További szerzők:	Khangembam, Rojesh (1991-) Farkas Róbert Oláh János (1976-) (állattenyésztés) Vass Nóra (1983-) (állatorvos) Monori István (1976-) (agrármérnök)
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