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001-es BibID:BIBFORM069248
Első szerző:Kusza Szilvia (agrármérnök)
Cím:Preliminary result of a genetic polymorphism of [beta]-lactoglobulin gene and the phylogenetic study of ten balkan and central european indigenous sheep breeds / Szilvia Kusza, Nikolett Sziszkosz, Krisztina Nagy, Amela Masala, Sándor Kukovics, Jávor András
Dátum:2015
ISSN:0001-527X
Megjegyzések:Genetic polymorphism at the [beta]-lactoglobulin ([beta]-LG) loci in indigenous sheep breeds (Tsigai, Racka, Pramenka) was determined. Altogether 904 sheep were genotyped for the presence of the A, B and C alleles of [beta]-lactoglobulin by PCR-RFLP. The AB genotype was the most common and the [beta]-lactoglobulin A was the most frequent in the Cokanski Tsigai (54%), while the B allele was the most common in the Rusty and the Zomborski Tsigai (59%, 60%). The C allele was found only in one individual from Serbian Cokanski flock. These results differ from those that refer to other native sheep breeds. In the Cokanski Tsigai, deviation from the Hardy-Weinberg equilibrium was detected. Genetic relationship based on [beta]-lactoglobulin polymorphism was the closest between the Rusty and the Cokanski Tsigai among the studied populations and between sheep and goat among the other ruminants. Part of the promoter region (254 bp) of [beta]-LG in studied sheep breeds were sequenced in order to identify polymorphisms, analyze haplotypes, and phylogenetic relationship among them. Sequencing analysis and alignment of the obtained sequences showed one haplotype. Analysis of more samples and longer parts of the promoter region of [beta]-LG are needed to reconstruct a phylogenetic tree.
Tárgyszavak:Agrártudományok Állattenyésztési tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Tsigai group
Zackel sheep
[beta]-LG
allele frequencies,
genetic relationship
sequencing
Megjelenés:Acta Biochimica Polonica 62 : 1 (2015), p. 109-112. -
További szerzők:Sziszkosz Nikolett (1988-) (molekuláris biológus) Nagy Krisztina (agrár) Masala, Amela Kukovics Sándor (1950-) (agrár) Jávor András (1952-) (agrármérnök)
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