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001-es BibID:	BIBFORM099490
035-os BibID:	(WoS)000747614400001 (Scopus)85121503950 (cikkazonosító)19
Első szerző:	Bencze János (orvos)
Cím:	Comparison of Semi-Quantitative Scoring and Artificial Intelligence Aided Digital Image Analysis of Chromogenic Immunohistochemistry / János Bencze, Máté Szarka, Balázs Kóti, Woosung Seo, Tibor G. Hortobágyi, Viktor Bencs, László V. Módis, Tibor Hortobágyi
Dátum:	2021
ISSN:	2218-273X
Megjegyzések:	Semi-quantitative scoring is a method that is widely used to estimate the quantity of proteins on chromogen-labelled immunohistochemical (IHC) tissue sections. However, it suffers from several disadvantages, including its lack of objectivity and the fact that it is a time-consuming process. Our aim was to test a recently established artificial intelligence (AI)-aided digital image analysis platform, Pathronus, and to compare it to conventional scoring by five observers on chromogenic IHC-stained slides belonging to three experimental groups. Because Pathronus operates on grayscale 0-255 values, we transformed the data to a seven-point scale for use by pathologists and scientists. The accuracy of these methods was evaluated by comparing statistical significance among groups with quantitative fluorescent IHC reference data on subsequent tissue sections. The pairwise inter-rater reliability of the scoring and converted Pathronus data varied from poor to moderate with Cohen's kappa, and overall agreement was poor within every experimental group using Fleiss' kappa. Only the original and converted that were obtained from Pathronus original were able to reproduce the statistical significance among the groups that were determined by the reference method. In this study, we present an AI-aided software that can identify cells of interest, differentiate among organelles, protein specific chromogenic labelling, and nuclear counterstaining after an initial training period, providing a feasible and more accurate alternative to semi-quantitative scoring.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk
Megjelenés:	Biomolecules. - 12 : 1 (2021), p. 1-14. -
További szerzők:	Szarka Máté (1990-) Kóti Balázs Seo, Woosung Hortobágyi Tibor G. (1998-) (orvostanhallgató) Bencs Viktor (1995-) (orvos) Módis László V. (neurológus) Hortobágyi Tibor (1965-) (patológus)
Pályázati támogatás:	EFOP-3.6.2-16-2017-00009 EFOP ÚNKP-21-3 Egyéb Nemzeti Agykutatási Program KTIA_13_NAP-A-II/7 Egyéb NKFIH_SNN_132999 Egyéb
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001-es BibID:	BIBFORM083135
035-os BibID:	(cikkazonosító)68 (scopus)85078993240 (wos)000519243400011
Első szerző:	Bencze János (orvos)
Cím:	Lemur tyrosine kinase 2 (LMTK2) level inversely correlates with phospho-tau in neuropathological stages of Alzheimer's disease / János Bencze, Máté Szarka, Viktor Bencs, Renáta Nóra Szabó, László V. Módis, Dag Aarsland, Tibor Hortobágyi
Dátum:	2020
Megjegyzések:	Alzheimer's disease (AD) is the most common neurodegenerative dementia. Mapping the pathomechanism and providing novel therapeutic options have paramount significance. Recent studies have proposed the role of LMTK2 in AD. However, its expression pattern and association with the pathognomonic neurofibrillary tangles (NFTs) in different brain regions and neuropathological stages of AD is not clear. We performed chromogenic (CHR) LMTK2 and fluorescent phospho-tau/LMTK2 double-labelling (FDL) immunohistochemistry (IHC) on 10-10 post-mortem middle frontal gyrus (MFG) and anterior hippocampus (aHPC) samples with early and late neuropathological Braak tau stages of AD. MFG in early stage was our ♭endogenous control' region as it is not affected by NFTs. Semi-quantitative CHR-IHC intensity scoring revealed significantly higher (p<0.001) LMTK2 values in this group compared to NFT-affected regions. FDL-IHC demonstrated LMTK2 predominance in the endogenous control region, while phospho-tau overburden and decreased LMTK2 immunolabelling were detected in NFT-affected groups (aHPC in early and both regions in late stage). Spearman's correlation coefficient showed strong negative correlation between phospho-tau/LMTK2 signals within each group. According to our results LMTK2 expression is inversely proportionate to the extent of NFT pathology, as well as decreased LMTK2 level is not a general feature in AD brain, rather it is characteristic to the NFT-affected regions.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk Alzheimer's disease LMTK2 neurodegeneration tau digital image analysis
Megjelenés:	Brain Sciences. - 10 : 2 (2020), p. 1-14. -
További szerzők:	Szarka Máté (1990-) Bencs Viktor (1995-) (orvos) Szabó Renáta Nóra Módis László V. (neurológus) Aarsland, Dag Hortobágyi Tibor (1965-) (patológus)
Pályázati támogatás:	EFOP-3.6.3-VEKOP-16-2017-00009 EFOP ÚNKP-19-3 Egyéb NAP (2017-1.2.1-NKP-2017-00002) Egyéb GINOP-2.3.2-15-2016-00043 GINOP ÚNKP-19-2 Egyéb
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001-es BibID:	BIBFORM081788
035-os BibID:	(cikkazonosító)17222 (WOS)000497712500018 (Scopus)85075476757
Első szerző:	Bencze János (orvos)
Cím:	Neuropathological characterization of Lemur tyrosine kinase 2 (LMTK2) in Alzheimer's disease and neocortical Lewy body disease / János Bencze, Máté Szarka, Viktor Bencs, Renáta Nóra Szabó, Máté Smajda, Dag Aarsland, Tibor Hortobágyi
Dátum:	2019
ISSN:	2045-2322
Megjegyzések:	Alzheimer's disease (AD) and neocortical Lewy body disease (LBD) are the most common neurodegenerative dementias, with no available curative treatment. Elucidating pathomechanism and identifying novel therapeutic targets is of paramount importance. Lemur tyrosine kinase 2 (LMTK2) is involved in several physiological and pathological cellular processes. Herewith a neuropathological characterization is presented in AD and neocortical LBD samples using chromogenic and fluorescent LMTK2 immunohistochemistry on post-mortem brain tissues and compared them to age-matched controls (CNTs). LMTK2 immunopositivity was limited to the neuronal cytoplasm. Neurons, including tau-positive tangle-bearing ones, showed decreased chromogenic and immunofluorescent labelling in AD in every cortical layer compared to CNT and neocortical LBD. Digital image analysis was performed to measure the average immunopositivity of groups. Mean grey values were calculated for each group after measuring the grey scale LMTK2 signal intensity of each individual neuron. There was significant difference between the mean grey values of CNT vs. AD and neocortical LBD vs. AD. The moderate decrease in neocortical LBD suggests the effect of coexisting AD pathology. We provide neuropathological evidence on decreased neuronal LMTK2 immunolabeling in AD, with implications for pathogenesis.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk
Megjelenés:	Scientific Reports. - 9 : 1 (2019), p. 1-9. -
További szerzők:	Szarka Máté (1990-) Bencs Viktor (1995-) (orvos) Szabó Renáta Nóra Smajda Máté Aarsland, Dag Hortobágyi Tibor (1965-) (patológus)
Pályázati támogatás:	EFOP-3.6.3-VEKOP-16-2017-00009 EFOP GINOP-2.3.2-15-2016-00043 GINOP ÚNKP-19-3 Egyéb ÚNKP-18-3 Egyéb ÚNKP-19-2 Egyéb 2017-1.2.1-NKP-2017-00002 MTA
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat DOI
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