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001-es BibID:BIBFORM111408
035-os BibID:(cikkazonosító)95 (scopus)85148518915 (wos)000936836300002
Első szerző:Járvás Gábor (vegyészmérnök)
Cím:Microbead-based extracorporeal immuno-affinity virus capture : a feasibility study to address the SARS-CoV-2 pandemic / Jarvas Gabor, Szerenyi Dora, Jankovics Hajnalka, Vonderviszt Ferenc, Tovari Jozsef, Takacs Laszlo, Foldes Fanni, Somogyi Balazs, Jakab Ferenc, Guttman Andras
Dátum:2023
ISSN:0026-3672
Megjegyzések:In this paper, we report on the utilization of micro-technology based tools to fight viral infections. Inspired by various hemoperfusion and immune-affinity capture systems, a blood virus depletion device has been developed that offers highly efficient capture and removal of the targeted virus from the circulation, thus decreasing virus load. Single-domain antibodies against the Wuhan (VHH-72) virus strain produced by recombinant DNA technology were immobilized on the surface of glass micro-beads, which were then utilized as stationary phase. For feasibility testing, the virus suspension was flown through the prototype immune-affinity device that captured the viruses and the filtered media left the column. The feasibility test of the proposed technology was performed in a Biosafety Level 4 classified laboratory using the Wuhan SARS-CoV-2 strain. The laboratory scale device actually captured 120,000 virus particles from the culture media circulation proving the feasibility of the suggested technology. This performance has an estimated capture ability of 15 million virus particles by using the therapeutic size column design, representing three times over-engineering with the assumption of 5 million genomic virus copies in an average viremic patient. Our results suggested that this new therapeutic virus capture device could significantly lower virus load thus preventing the development of more severe COVID-19 cases and consequently reducing mortality rate.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Microchimica Acta. - 190 : 3 (2023), p. 1-9. -
További szerzők:Szerényi Dóra Jankovics Hajnalka Vonderviszt Ferenc Tóvári József Takács László (1955-) (orvos) Földes Fanni Vivien (virológus) Somogyi Balázs Jakab Ferenc (1977-) (biológus) Guttman András (1954-) (vegyészmérnök)
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001-es BibID:BIBFORM099695
035-os BibID:(cikkazonosító)338892
Első szerző:Reider Balázs
Cím:Integrated workflow for urinary prostate specific antigen N-glycosylation analysis using sdAb partitioning and downstream capillary electrophoresis separation / Reider Balazs, Gacsi Eszter, Jankovics Hajnalka, Vonderviszt Ferenc, Szarvas Tibor, Guttman Andras, Jarvas Gabor
Dátum:2021
ISSN:0003-2670
Megjegyzések:Prostate cancer represents the second highest malignancy rate in men in all cancer diagnoses worldwide. The development and progression of prostate cancer is not completely understood yet at molecular level, but it has been reported that changes in the N-glycosylation of prostate-specific antigen (PSA) occur during tumor genesis. In this paper we report on the development and implementation of a highthroughput capillary electrophoresis based glycan analysis workflow for urinary PSA analysis. The technology utilizes selective, high yield single domain antibody based PSA capture, followed by preconcentration and capillary electrophoresis coupled with laser-induced fluorescence detection, resulting in high resolution N-glycan profiles. Urinary PSA glycan profiles were compared to a commercially available PSA standard revealing differences in their a2,3- and a2,6-sialylated isomers, proving the excellent selectivity of the suggested workflow. This is important as sialylation classification plays an important role in the differentiation between indolent, significant and aggressive forms of prostate cancer.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Analytica Chimica Acta. - 1184 (2021), p. 1-10. -
További szerzők:Gacsi Eszter Jankovics Hajnalka Vonderviszt Ferenc Szarvas Tibor (matematikus informatikus) Guttman András (1954-) (vegyészmérnök) Járvás Gábor (1982-) (vegyészmérnök)
Pályázati támogatás:2018e2.1.17- T ET-KR-2018-00010
Egyéb
UNKP-20-5
Egyéb
TKP2020-IKA-07
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DOI
Intézményi repozitóriumban (DEA) tárolt változat
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