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001-es BibID:BIBFORM071421
Első szerző:Biró Orsolya (molekuláris biológus)
Cím:Various levels of circulating exosomal total-miRNA and miR-210 hypoxamiR in different forms of pregnancy hypertension / Orsolya Biró, Bálint Alasztics, Attila Molvarec, József Joó, Bálint Nagy, János Rigó Jr.
Dátum:2017
ISSN:2210-7789
Megjegyzések:IntroductionHypertension is a common complication during pregnancy, affecting 10% of pregnant women worldwide. Several microRNA (miRNA) were shown to be involved in hypertensive disorders of pregnancy. In preeclampsia (PE), placental dysfunction causes the enhanced release of extracellular vesicle-derived miRNAs. The hypoxia-sensitive hsa-mir-210 is the most common PE-associated miRNA, but its exosomal profile has not been investigated.ObjectivesOur aims were to measure exosomal total-miRNA concentration and to perform expression analysis of circulating exosomal hsa-miR-210 in women affected by chronic hypertension (CHT) gestational hypertension (GHT) or PE.Materials and methodsWe collected plasma samples from women with CHT, GHT, PE (moderate: mPE and severe: sPE) and from normotensive pregnancies. Exosomal miRNAs were extracted and miRNA concentration was measured. RT-PCR was carried out with hsa-miR-210-3p-specific primers and relative expression was calculated using the comparative Ct method.ResultsThe total-miRNA concentration was different in the disease subgroups, and was significantly higher in mPE and sPE compared to the other groups. We found a significant difference in the relative exosomal hsa-miR-210-3p expression between all hypertensive groups compared to the normotensive samples, but significant upregulation was only observed in case of mPE and sPE patients. Both the level of total-miRNA and hsa-miR-210 expression was higher in case of severe PE.ConclusionsThe level of circulating exosomal total-miRNA and hsa-miR-210 was elevated in women with PE, and it was higher in the severe form. We showed that hsa-miR-210 is secreted via exosomes, which may have a role in the pathomechanism of the disease.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
miRNA
miR-210
circulating
Megjelenés:Pregnancy Hypertension 10 (2017), p. 207-212. -
További szerzők:Alasztics Bálint Molvarec Attila (szülész-nőgyógyász) Joó József Gábor (1965-) (szülész-nőgyógyász) Nagy Bálint (1956-) (molekuláris genetikus) Rigó János (1958-) (szülész-nőgyógyász)
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2.

001-es BibID:BIBFORM066489
Első szerző:Biró Orsolya (molekuláris biológus)
Cím:Identifying miRNA regulatory mechanisms in preeclampsia by systems biology approaches / Biró Orsolya, Nagy Bálint, Rigó János
Dátum:2016
ISSN:1064-1955
Megjegyzések:BACKGROUND: Preeclampsia (PE) is the major cause of maternal and fetal morbidity and mortality, affecting 3-8% of all pregnancies around the globe. miRNAs are small, noncoding RNA molecules, which negatively regulate gene expression. Abnormally expressed miRNAs contribute to pregnancy complications such as PE. The aim of our study was to find possible regulatory mechanisms by system biology approaches, which are connected to the pathogenesis of PE.METHODS: We integrated publicly available miRNA and gene expression profiles and created a network from the significant miRNA-mRNA pairs with the help of MAGIA and Cytoscape softwares. Two subnetworks were expanded by adding protein-protein interactions. Differentially expressed miRNAs were identified for the evaluation of their regulatory effect. We analyzed the miRNAs and their targets using different bioinformatics tools and through literature research.RESULTS: Altogether, 52,603 miRNA-mRNA interactions were generated by the MAGIA web tool. The top 250 interactions were visualized and pairs with q < 0.0001 were analyzed, which included 85 nodes and 80 edges signalizing the connections between 52 regulated genes and 33 miRNAs. A total of 11 of the regulated genes are PE related and 9 of them were targeted by multiple miRNAs. A total of 8 miRNAs were associated with PE before, and 13 miRNAs regulated more than 1 mRNA. Hsa-mir-210 was the highest degree node in the network and its role in PE is well established.CONCLUSIONS: We identified several miRNA-mRNA regulatory mechanisms which may contribute to the pathogenesis of PE. Further investigations are needed to validate these miRNA-mRNA interactions and to enlighten the possibilities of developing potential therapeutic targets.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
preeclampsia
miRNA
systems
regulatory
Megjelenés:Hypertension In Pregnancy 35 (2016), p. 1-10. -
További szerzők:Nagy Bálint (1956-) (molekuláris genetikus) Rigó János (1958-) (szülész-nőgyógyász)
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3.

001-es BibID:BIBFORM075326
Első szerző:Hupuczi Petronella (anaesthesiológus)
Cím:Characteristic laboratory changes in pregnancies complicated by HELLP syndrome / Petronella Hupuczi, Bálint Nagy, István Sziller, Barbara Rigó, Ervin Hruby, Zoltán Papp
Dátum:2007
ISSN:1064-1955
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Hypertension In Pregnancy. - 26 : 4 (2007), p. 389-401. -
További szerzők:Nagy Bálint (1956-) (molekuláris genetikus) Sziller István (szülész-nőgyógyász szakorvos) Rigó Barbara (szülész-nőgyógyász) Hruby Ervin Papp Zoltán (1942-) (szülész-nőgyógyász, genetikus)
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4.

001-es BibID:BIBFORM074861
Első szerző:Rigó János (szülész-nőgyógyász)
Cím:Maternal and neonatal outcome of preeclamptic pregnancies : the potential roles of factor V Leiden mutation and 5,10 methylenetetrahydrofolate reductase / Rigó J. Jr, Nagy B., Fintor L., Tanyi J., Beke A., Karádi I., Papp Z.
Dátum:2000
ISSN:1064-1955
Megjegyzések:OBJECTIVE:To investigate the potential perinatal effects of Factor V Leiden mutation and 5,10 methylenetetrahydrofolate reductase C677T polymorphism in preeclamptic women.STUDY DESIGN:One hundred twenty preeclamptic women (N = 120) and 101 healthy pregnant controls (N = 101) were recruited and evaluated for frequency of Leiden and 5,10 methylenetetrahydrofolate reductase (MTHFR) mutations using polymerase chain reaction (PCR). Perinatal outcomes were then recorded and analyzed for all study participants and their neonates.RESULTS:Laboratory analysis yielded 22 (18.33%) heterozygous carriers of Factor V Leiden mutation among preeclamptic women and 3 (2.97%) heterozygous carriers among the healthy controls; differences between the two groups were found to be statistically significant [p < 0.001, the relative risk (RR) = 6.17, 95% confidence interval (95% CI) = 1.90-20.02]. Homozygous MTHFR mutations were found in 8 of 120 (6.67%) preeclamptic women and in 6 of the 101 (5.94%) healthy controls evaluated. Among preeclamptic women, episodes of hemolysis, elevated liver enzymes, and low platelet (HELLP) syndrome were reported in 7 of 22 (31.81%) of those with Factor V Leiden mutation and in 11 of 98 (11.22%) of those who were negative for the mutation. Group differences were determined to be statistically significant (p < 0.015, RR = 2.83, 95% CI = 1.24-6. 48). Perinatal indicators collected from the two groups included frequency of intrauterine growth retardation, birth weight, and gestational age. No statistically different perinatal outcomes were found between Factor V Leiden positive and negative preeclamptic women. In addition, MTHFR gene polymorphism did not appear to be correlated with the development of preeclampsia.CONCLUSION:Although the frequency of Factor V Leiden mutation appears to be significantly higher among preeclamptic women, the mechanism of pathogenesis and potential influence on perinatal outcomes is not yet well understood. Relatively high rates of HELLP syndrome among those with Factor V Leiden mutation suggest that this thrombogene mutation may play a significant role in hemostatic system activation. Our results suggest that the role of MTHFR polymorphism and other factors such as folic acid supplementation will require more extensive analysis in controlling worldwide morbidity and mortality associated with this important maternal condition.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Leiden mutation
MTHFR
preeclampsia
Megjelenés:Hypertension In Pregnancy. - 19 : 2 (2000), p. 163-172. -
További szerzők:Nagy Bálint (1956-) (molekuláris genetikus) Fintor, Lou Tanyi János Beke Artúr (szülész-nőgyógyász) Karádi István (1952-) (belgyógyász, kardiológus) Papp Zoltán (1942-) (szülész-nőgyógyász, genetikus)
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