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1.

001-es BibID:BIBFORM071421
Első szerző:Biró Orsolya (molekuláris biológus)
Cím:Various levels of circulating exosomal total-miRNA and miR-210 hypoxamiR in different forms of pregnancy hypertension / Orsolya Biró, Bálint Alasztics, Attila Molvarec, József Joó, Bálint Nagy, János Rigó Jr.
Dátum:2017
ISSN:2210-7789
Megjegyzések:IntroductionHypertension is a common complication during pregnancy, affecting 10% of pregnant women worldwide. Several microRNA (miRNA) were shown to be involved in hypertensive disorders of pregnancy. In preeclampsia (PE), placental dysfunction causes the enhanced release of extracellular vesicle-derived miRNAs. The hypoxia-sensitive hsa-mir-210 is the most common PE-associated miRNA, but its exosomal profile has not been investigated.ObjectivesOur aims were to measure exosomal total-miRNA concentration and to perform expression analysis of circulating exosomal hsa-miR-210 in women affected by chronic hypertension (CHT) gestational hypertension (GHT) or PE.Materials and methodsWe collected plasma samples from women with CHT, GHT, PE (moderate: mPE and severe: sPE) and from normotensive pregnancies. Exosomal miRNAs were extracted and miRNA concentration was measured. RT-PCR was carried out with hsa-miR-210-3p-specific primers and relative expression was calculated using the comparative Ct method.ResultsThe total-miRNA concentration was different in the disease subgroups, and was significantly higher in mPE and sPE compared to the other groups. We found a significant difference in the relative exosomal hsa-miR-210-3p expression between all hypertensive groups compared to the normotensive samples, but significant upregulation was only observed in case of mPE and sPE patients. Both the level of total-miRNA and hsa-miR-210 expression was higher in case of severe PE.ConclusionsThe level of circulating exosomal total-miRNA and hsa-miR-210 was elevated in women with PE, and it was higher in the severe form. We showed that hsa-miR-210 is secreted via exosomes, which may have a role in the pathomechanism of the disease.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
miRNA
miR-210
circulating
Megjelenés:Pregnancy Hypertension 10 (2017), p. 207-212. -
További szerzők:Alasztics Bálint Molvarec Attila (szülész-nőgyógyász) Joó József Gábor (1965-) (szülész-nőgyógyász) Nagy Bálint (1956-) (molekuláris genetikus) Rigó János (1958-) (szülész-nőgyógyász)
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2.

001-es BibID:BIBFORM065207
Első szerző:Fazakas Ádám
Cím:Genetic predisposition in patients with hypertension and normal ejection fraction to oxidative stress / Fazakas Ádám, Szelényi Zsuzsanna, Szénási Gábor, Nyírő Gábor, Szabó Péter M., Patócs Attila, Tegze Narcis, Fekete Bertalan C., Molvarec Attila, Nagy Bálint, Jakus Judit, Örsi Ferenc, Karádi István, Vereckei András
Dátum:2016
ISSN:1933-1711 1878-7436
Megjegyzések:The role of oxidative stress (OXS) due to myocardial nitric oxide synthase (NOS) uncoupling related to oxidative depletion of its cofactor tetrahydrobiopterin (BH4) emerged in the pathogenesis of heart failure with preserved ejection fraction. We determined the prevalence of six single nucleotide polymorphisms (SNPs) of genes encoding enzymes related to OXS, BH4 metabolism, and NOS function in ?60-year-old 94 patients with hypertension and 18 age-matched controls with normal ejection fraction. Using echocardiography, 56/94 (60%) patients with hypertension had left ventricular (LV) diastolic dysfunction (HTDD+ group) and 38/94 (40%) patients had normal LV diastolic function (HTDD? group). Four SNPs (rs841, rs3783641, rs10483639, and rs807267) of guanosine triphosphate cyclohydrolase-1, the rate-limiting enzyme in BH4 synthesis, one (rs4880) of manganese superoxide dismutase, and one (rs1799983) of endothelial NOS genes were genotyped using real-time polymerase chain reaction method and Taqman probes. Protein carbonylation, BH4, and total biopterin levels were measured from plasma samples. No between-groups difference in minor allele frequency of SNPs was found. We calculated a genetic score indicating risk for OXS based on the minor allele frequencies of the SNPs. A high genetic risk for OXS was significantly associated with HTDD+ even after adjustment for confounding variables (odds ratio [95% confidence interval]:4.79 [1.12?20.54]; P = .035). In both patient groups protein carbonylation (P < .05 for both), plasma BH4 (P < .01 for both) and in the HTDD+ group total biopterin (P < .05) increased versus controls. In conclusion, in patients with hypertension and normal ejection fraction, a potential precursor of heart failure with preserved ejection fraction, a partly genetically determin
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
heart failure
Hypertension
oxidative stress
Megjelenés:Journal of the American Society of Hypertension 10 : 2 (2016), p. 124-132. -
További szerzők:Szelényi Zsuzsanna Szénási Gábor Nyírő Gábor (molekuláris biológus) Szabó Péter M. Patócs Attila Tegze, Narcis Fekete Bertalan C. Molvarec Attila (szülész-nőgyógyász) Nagy Bálint (1956-) (molekuláris genetikus) Jakus Judit Örsi Ferenc Karádi István (1952-) (belgyógyász, kardiológus) Vereckei András
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3.

001-es BibID:BIBFORM073617
Első szerző:Gullai Nóra
Cím:Evaluation of a rapid and simple placental growth factor test in hypertensive disorders of pregnancy / Nóra Gullai, Balázs Stenczer, Attila Molvarec, Gergely Fügedi, Zoltán Veresh, Bálint Nagy, János Rigó Jr.
Dátum:2013
ISSN:0916-9636
Megjegyzések:The aim of this study was to investigate the diagnostic accuracy of the Triage placental growth factor (PlGF) assay, together with its prognostic efficiency in determining the need for preterm delivery in all forms of hypertensive disorders of pregnancy. A total of 130 pregnant women with a diagnosis of preeclampsia (PE: 23), HELLP syndrome (20), superimposed preeclampsia (SIPE: 17), chronic hypertension (CHT: 25), gestational hypertension (GHT: 18) and 27 normotensive pregnant controls were enrolled in this case-control study. A single blood sample was taken between 22 and 34 weeks of gestation, and the plasma was analyzed for PlGF using the Alere Triage PlGF assay. The PlGF levels found in all hypertensive disorder groups differed significantly from those observed in controls. There was a highly significant difference in PlGF concentrations between women with a pregnancy duration <35 weeks and controls. Using a gestational age-dependent threshold of 5% of normal, a positive PlGF test predicted delivery before 35 weeks in 93.7% of hypertensive women and delivery before 37 weeks in 90.5% of hypertensive women. A positive PlGF test identified the following proportions of hypertensive patients: 95.7% (PE), 95.0% (HELLP syndrome), 82.4% (SIPE), 60.0% (CHT) and 44.4% (GHT). A positive PlGF test was associated with a significantly shorter duration of pregnancy (hazard ratio of 3.43 adjusted for the gestational age at the time of sample collection and hypertension with proteinuria). In conclusion, PlGF concentrations are significantly lower in all hypertensive disorders. A positive test using the Triage PlGF assay at 22-34 weeks of gestation predicts delivery before 37 weeks in women with both proteinuric and non-proteinuric hypertensive disorders of pregnancy.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
hypertension
pregnancy
placental
growth
Megjelenés:Hypertension Research 36 : 5 (2013), p. 457-462. -
További szerzők:Stenczer Balázs Molvarec Attila (szülész-nőgyógyász) Fügedi Gergely Veresh Zoltán Nagy Bálint (1956-) (molekuláris genetikus) Rigó János (1958-) (szülész-nőgyógyász)
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4.

001-es BibID:BIBFORM065236
Első szerző:Lázár Levente (szülész-nőgyógyász)
Cím:Relationship of circulating cell-free DNA levels to cell-free fetal DNA levels, clinical characteristics and laboratory parameters in preeclampsia / Levente Lazar, János Rigó Jr., Bálint Nagy, Krisztián Balogh, Veronika Makó, László Cervenak, Miklós Mézes, Zoltán Prohászka, Attila Molvarec
Dátum:2009
ISSN:1471-2350
Megjegyzések:Background:The aim of our study was to examine whether increased circulating total cell-freeDNA levels are related to the clinical characteristics and standard laboratory parameters ofpreeclamptic patients, to markers of inflammation, endothelial activation or injury, oxidative stressand to cell-free fetal DNA levels.Methods:Circulating total cell-free DNA was measured by real-time quantitative PCR in plasmasamples obtained from 67 preeclamptic and 70 normotensive pregnant women. Standardlaboratory parameters, C-reactive protein, plasma von Willebrand factor antigen, plasmafibronectin, plasma malondialdehyde and cell-free fetal DNA levels were also determined.Results and Conclusion:Circulating total cell-free and fetal deoxyribonucleic acid levels weresignificantly elevated in pregnancies complicated by preeclampsia (median: 11.395 vs. 32.460 and0.001 vs. 0.086 pg/?l; P < .001). The quantity of plasma total cell-free DNA did not correlate with most of the laboratory parameters, except for serum aspartate aminotransferase and alanineaminotransferase activities (correlation coefficient: 0.31; P = 0.012 and 0.46; P < .001). There was no correlation with clinical characteristics, including body mass index. The releases of both free fetal and total cell-free deoxyribonucleic acid were found to be affected in preeclampsia.Hepatocellular necrosis seems to be responsible - at least partly - for increased circulating total DNA levels in preeclampsia, as suggested by the significant correlation with liver enzyme activities
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
cell free
DNA
Preeclampsia
clinical
Characteristics
Megjelenés:BMC Medical Genetics. - 10 (2009), p. 1-6. -
További szerzők:Rigó János (1958-) (szülész-nőgyógyász) Nagy Bálint (1956-) (molekuláris genetikus) Balogh Krisztián Makó Veronika Cervenak László Mézes Miklós Prohászka Zoltán Molvarec Attila (szülész-nőgyógyász)
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Intézményi repozitóriumban (DEA) tárolt változat
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5.

001-es BibID:BIBFORM065234
Első szerző:Lázár Levente (szülész-nőgyógyász)
Cím:A vérplazma összes szabad DNS, valamint szabad magzati DNS mennyisége praeeclampsiával szövődött és szövődménymentes terhességek esetében / Lázár Levente, Nagy Bálint, Molvarec Attila, Rigó János Jr.
Dátum:2010
ISSN:0030-6002 1788-6120
Megjegyzések:A plazmában keringő szabad DNS minőségi és mennyiségi vizsgálata mint a praenatalis diagnosztika lehetséges eszköze egy évtizedes múltra tekint vissza. Az általunk végzett vizsgálat célja az összes szabad DNS, valamint szabad magzati DNS mennyiségének vizsgálata volt szövődménymentes, illetve praeeclampsiával szövődött terhességek esetén. Módszerek:A vizsgálatot retrospektív módon végeztük. Klinikánkon megjelent és kezelés alatt álló 67 praeeclampsiával szövődött, illetve 70 szövődménymentes terhességet viselő várandós nőtől gyűjtöttünk vérplazmát. A keringő összes szabad DNS és szabad magzati DNS meghatározását valós idejű polimeráz láncreakcióval (real-time PCR) végeztük. Eredmények:A plazma szabad DNS, valamint a szabad magzati DNS mennyisége egyaránt szignifikánsan magasabb volt praeeclampsiás terhesek esetében (medián: 0,0114 vs. 0,0325 és 0,001 E-3 vs. 0,086 E-3 ng/?l; P <0,001). Az összes szabad DNS, valamint a szabad magzati DNS mennyisége között, illetve a testtömegindex között nem találtunk szignifikáns összefüggést. Következtetés:A praeeclampsia hátterében feltételezett kórfolyamatok, a placentáció zavara, endothel- és hepatocellularis károsodás nagy valószínűséggel egyaránt befolyásolja az összes szabad DNS mennyiségének emelkedését. A szabad magzati DNS mennyiségének emelkedése a trophoblastinvázió zavarának mutatójaként lehetséges markerként jön szóba a praeeclampsia korai diagnosztizálásában
Tárgyszavak:Orvostudományok Klinikai orvostudományok magyar nyelvű folyóiratközlemény hazai lapban
szabad nukleinsav
Terhesség
mennyiség
Megjelenés:Orvosi Hetilap 151 : 19 (2010), p. 784-787. -
További szerzők:Nagy Bálint (1956-) (molekuláris genetikus) Molvarec Attila (szülész-nőgyógyász) Rigó János (1958-) (szülész-nőgyógyász)
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6.

001-es BibID:BIBFORM065224
Első szerző:Lázár Levente (szülész-nőgyógyász)
Cím:Role of hsa-miR-325 in the etiopathology of preeclampsia / Levente Lázár, Bálint Nagy, Attila Molvarec, András Szarka, János Rigó
Dátum:2012
ISSN:1791-2997 1791-3004
Megjegyzések:Preeclampsia (PE) is a common pregnancy-specific syndrome characterized by hypertension and proteinuria. Evidence has demonstrated that hypertensive disorders in pregnancy are associated with alterations in the expression of different microRNAs (miRNAs). miRNAs are endogenously expressed non-coding RNAs that have significant biological and pathological functions due to their potential mechanisms of regulation of gene expression. The purpose of the present study was to investigate the expression of hsa-miR-325 in placental samples of preeclamptic and uncomplicated pregnancy patients. hsa-miR-325 was isolated from placenta tissue samples obtained from 31 preeclamptic and 28 normotensive pregnant females. Quantitative real-time polymerase chain reaction was used to analyze miRNA expression. The expression of hsa-miR?325 was elevated in uncomplicated pregnancies compared with preeclamptic patients. ?Ct (mean ? SD) values were 0.117?0.07 in PE tissues and 0.135?0.051 in normotensive cases (p<0.05). The expression levels correlated with patient blood pressure (p=0.015, r=-0.23), and tended to correlate with body mass index (p=0.065, r=0.261). The expression of hsa-miR-325 was downregulated in the case of PE. Changes in hsa-miR?325 expression in the case of pregnancy-related hypertensive disorders might affect the oxidative stress pathways and heat-shock protein production. These factors have a strong correlation with the development of PE. We, therefore, suggest that hsa-miR-325 contributes to the pathogenesis of PE.IntroductionHypertensive disorders are a leading cause of perinatal morbidity and mortality in pregnancy. Preeclampsia (PE) is a common pregnancy-specific syndrome that affects at least 5% of all pregnancies worldwide (1,2). It is characterized by hypertension (RR ?140/90 mmHg) and proteinuria of ?300 mg/24 h, developing after midgestation in previously normotensive pregnant females. Although the exact etiology of PE remains unknown, pathological studies have demonstrated the abnormal development of an ischemic placenta (2). Shallow endovascular trophoblast invasion in the spiral arteries and generalized endothelial cell dysfunction are key factors, while placental ischemia, oxidative stress and maternal-fetal immune maladaptation affect the development of PE. The only treatment is delivery of the placenta, after which the symptoms regress rapidly. Evidence has demonstrated that hypertensive disorders in non-pregnant and pregnant patients are associated with alterations in different miRNA expressions of specific tissues (3?5).miRNAs are non-protein coding RNAs, and functionally negative regulators of gene expression by antisense complementarity to specific messenger RNAs (6,7). They act by targeting the RNA-induced silencing complex to complementary sites within the 3·-untranslated region (UTR) of their target mRNAs. Depending on the degree of base pairing between the miRNA and the 3·-UTR, either degradation or translational repression of the targeted mRNA occurs. Although they account for less than 1% of all human genes, miRNAs have been estimated to regulate up to 30% of all protein-encoding genes (8). Their best-known representatives are the 18?24 nucleotide long single-stranded miRNA. The mean number of copies per cell is between 10 and 50000, depending on the tissue and the miRNA (9,10).Few studies have presented data regarding a comprehensive list of the human miRNAs expressed in the placenta and the possible roles of the different miRNAs in the pathophysiology of pregnancy-related disorders (11?13). Taking into consideration the high number of miRNAs, further studies regarding the expression of different miRNA in pregnancy-related hypertensive disorders may be important in understanding the pathophysiology of this disease.In the present study, we performed a real-time polymerase chain reaction PCR analysis of hsa-miR-325. hsa-miR-325 is located at Xq21.1. Different databases (http://www.mirdb.org; http://www.nextprot.org) were evaluated, and hsa-miR-325 was selected as it is a non-studied miRNA that targets genes and candidate protein regulatory pathways affecting different etiological factors, including body mass index (BMI), blood pressure regulation, oxidative stress, endometrial function and heat-shock protein regulation, thus playing a key role in the development of hypertensive disorders in pregnancy.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
miRNA
Preeclampsia
gene expression
Megjelenés:Molecular Medicine Reports 6 (2012), p. 597-600. -
További szerzők:Nagy Bálint (1956-) (molekuláris genetikus) Molvarec Attila (szülész-nőgyógyász) Szarka András Rigó János (1958-) (szülész-nőgyógyász)
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7.

001-es BibID:BIBFORM065298
Első szerző:Madách Krisztina
Cím:Elevated serum 70 kDa heat shock protein level reflects tissue damage and disease severity in the syndrome of hemolysis, elevated liver enzymes, and low platelet count / Krisztina Madách, Attila Molvarec, János Rigó Jr., Bálint Nagy, István Pénzes, István Karádi, Zoltán Prohászka
Dátum:2008
ISSN:0301-2115
Megjegyzések:We have recently demonstrated that serum 70 kDa heat shock protein (Hsp70) levels are increased in the syndrome of hemolysis, elevated liver enzymes, and low platelet count (HELLP syndrome). The aim of the present study was to investigate in an independent, larger cohort of patients whether serum Hsp70 levels are related to laboratory markers of HELLP syndrome.STUDY DESIGN:The study population included 14 patients with HELLP syndrome. Serum Hsp70 levels were measured by enzyme-linked immunosorbent assay. The relationship between serum Hsp70 levels and laboratory markers of hemolysis, hepatocellular damage, renal insufficiency, inflammation or disseminated intravascular coagulation (DIC), as well as platelet count was investigated by calculating correlation coefficients, standardized regression coefficients and by principal component analysis.RESULTS:Serum Hsp70 levels showed a very strong correlation to the markers of hemolysis (plasma free hemoglobin level, serum lactate dehydrogenase activity, and total bilirubin level) and of hepatocellular injury (serum aminotransferase activities), supported also by principal component analysis. Furthermore, circulating Hsp70 concentration reflected the severity of HELLP syndrome as expressed by the significant inverse correlation to the lowest platelet count. By contrast, there was no relationship between serum Hsp70 levels and markers of inflammation, coagulation, fibrinolysis or renal insufficiency.CONCLUSION:Elevated serum 70 kDa heat shock protein level seems to reflect tissue damage (hemolysis and hepatocellular injury) and disease severity in patients with HELLP syndrome. However, further investigations are needed to determine the clinical relevance of these findings.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
heat shock
elevated
HELLP syndrome
Megjelenés:European Journal Of Obstetrics Gynecology And Reproductive Biology 139 : 2 (2008), p. 133-138. -
További szerzők:Molvarec Attila (szülész-nőgyógyász) Rigó János (1958-) (szülész-nőgyógyász) Nagy Bálint (1956-) (molekuláris genetikus) Pénzes István Karádi István (1952-) (belgyógyász, kardiológus) Prohászka Zoltán
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8.

001-es BibID:BIBFORM065241
Első szerző:Molvarec Attila (szülész-nőgyógyász)
Cím:Circulating anti-heat-shock-protein antibodies in normal pregnancy and preeclampsia / Attila Molvarec, Zoltán Derzsy, Judit Kocsis, Tamás Bőze, Bálint Nagy, Krisztián Balogh, Veronika Makó, László Cervenak, Miklós Mézes, István Karádi, Zoltán Prohászka, János Rigó Jr.
Dátum:2009
ISSN:1355-8145
Megjegyzések:It has been previously reported that circulating anti-heat-shock-protein (Hsp) antibody levels are elevated in cardiovascular disorders. The aim of the present study was to determine circulating antihuman Hsp60, antimycobacterial Hsp65, and antihuman Hsp70 antibody levels in healthy pregnant women and preeclamptic patients and to investigate their relationship to the clinical characteristics of the study subjects, as well as to the markers of inflammation (C-reactive protein (CRP)), endothelial activation (von Willebrand factor antigen), or endothelial injury (fibronectin), oxidative stress (malondialdehyde) and to serum Hsp70 levels. Ninety-three preeclamptic patients and 127 normotensive healthy pregnant women were involved in this case control study. Serum anti-Hsp60, anti-Hsp65, anti-Hsp70, and Hsp70 levels were measured with enzyme-linked immunosorbent assay (ELISA). Serum CRP levels were determined by an autoanalyzer using the manufacturer's kit. Plasma von Willebrand factor antigen levels were quantified by ELISA, while plasma fibronectin concentration by nephelometry. Plasma malondialdehyde levels were measured by the thiobarbituric-acid-based colorimetric assay. For statistical analyses, nonparametric methods were applied. Anti-Hsp60, anti-Hsp65, and anti-Hsp70 antibodies were detected in all of our serum samples. There were no significant differences in serum anti-Hsp60, anti-Hsp65, and anti-Hsp70 antibody levels between the control and preeclamptic groups. Serum levels of Hsp70 and CRP, as well as plasma levels of VWF antigen, fibronectin, and malondialdehyde, were significantly higher in preeclamptic patients than in normotensive healthy pregnant women. Serum anti-Hsp60 antibody levels showed significant correlations with serum anti-Hsp65 antibody levels both in the control and the preeclamptic groups (Spearman R?=?0.55 and 0.59; p?<?0.001, respectively). However, no other relationship was found between clinical features (maternal age, smoking status, parity, body mass index, gestational age at blood draw, systolic and diastolic blood pressure, gestational age at delivery, and fetal birth weight) and measured laboratory parameters of the study subjects and serum anti-Hsp antibody levels in either study group. In conclusion, anti-Hsp60 and anti-Hsp70 antibodies as naturally occurring autoantibodies are present in the peripheral circulation of healthy pregnant women. Nevertheless, humoral immunity against heat shock proteins was not associated with preeclampsia. Further studies are warranted to explore the role of heat shock proteins and immune reactivity to them in the immunobiology of normal pregnancy and preeclampsia.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
circulating
anti heatschock
protein
preeclampsia
Megjelenés:Cell Stress & Chaperones. - 14 : 5 (2009), p. 491-498. -
További szerzők:Derzsy Zoltán Kocsis Judit (1967-) (onkológus) Bőze Tamás Nagy Bálint (1956-) (molekuláris genetikus) Balogh Krisztián Makó Veronika Cervenak László Mézes Miklós Karádi István (1952-) (belgyógyász, kardiológus) Prohászka Zoltán Rigó János (1958-) (szülész-nőgyógyász)
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9.

001-es BibID:BIBFORM065232
Első szerző:Molvarec Attila (szülész-nőgyógyász)
Cím:Circulating angiogenic factors determined by electrochemiluminescence immunoassay in relation to the clinical features and laboratory parameters in women with pre-eclampsia / Attila Molvarec, András Szarka, Szilvia Walentin, Endre Szűcs, Bálint Nagy, János Rigó Jr.
Dátum:2010
ISSN:0916-9636
Megjegyzések:The purpose of this study was to determine whether increased serum soluble fms-like tyrosine kinase-1 (sFlt-1) and decreasedplacental growth factor (PlGF) levels in pre-eclampsia are related to the clinical features and laboratory parameters of thepatients, including markers of inflammation, endothelial activation and injury, oxidative stress and trophoblast debris. A total of54 pre-eclamptic patients, 58 healthy pregnant and 52 healthy non-pregnant women were involved in this case?control study.Serum sFlt-1 and PlGF levels were measured by electrochemiluminescence immunoassay. Serum levels of sFlt-1 and PlGF weresignificantly higher in pre-eclamptic patients and healthy pregnant women than in healthy non-pregnant women. In addition,pre-eclamptic patients had significantly higher sFlt-1 levels and significantly lower PlGF concentrations compared with healthy pregnant women. According to the subgroup analyses, sFlt-1 levels were significantly higher in severely pre-eclamptic patients than in those with mild pre-eclampsia, whereas pre-eclamptic patients with fetal growth restriction or preterm onset of the disease had significantly lower PlGF concentrations compared with those without intrauterine growth restriction or with a diseaseonset at term. In the pre-eclamptic group, there were significant positive correlations between serum sFlt-1 levels and systolic and diastolic blood pressure, serum levels of blood urea nitrogen and creatinine, as well as plasma levels of von Willebrand factor antigen, fibronectin and cell-free fetal DNA. Furthermore, serum PlGF concentrations of pre-eclamptic patients showedsignificant positive correlations with gestational age at disease onset and delivery, as well as with fetal birth weight, and significant inverse correlations with levels of blood urea nitrogen, creatinine and fibronectin. In conclusion, increased serum sFlt-1 and decreased PlGF levels are associated with blood pressure, renal and endothelial dysfunction, trophoblast deportation, as well as with a shorter duration of pregnancy, fetal growth restriction, the severity and preterm onset of the disease in pre-eclampsia. These findings indicate the central role of an angiogenic imbalance in the pathogenesis of this pregnancy-specific disorde
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
circulating
Angiogenic
preeclampsia
factors
Megjelenés:Hypertension Research 33 : 9 (2010), p. 892-898. -
További szerzők:Szarka András Walentin Szilvia Szűcs Endre Nagy Bálint (1956-) (molekuláris genetikus) Rigó János (1958-) (szülész-nőgyógyász)
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Intézményi repozitóriumban (DEA) tárolt változat
Borító:

10.

001-es BibID:BIBFORM065219
Első szerző:Molvarec Attila (szülész-nőgyógyász)
Cím:Comparison of placental growth factor and fetal flow Doppler ultrasonography to identify fetal adverse outcomes in women with hypertensive disorders of pregnancy : an observational study / Attila Molvarec, Nóra Gullai, Balázs Stenczer, Gergely Fügedi, Bálint Nagy, János Rigó Jr.
Dátum:2013
Megjegyzések:Background:Hypertensive disorders of pregnancy and intrauterine growth restriction (IUGR) are leading causes ofmaternal and perinatal morbidity and mortality. Failure to detect intrauterine growth restriction in women at highrisk has been highlighted as a significant avoidable cause of serious fetal outcome. In this observational study wecompare fetal flow using Doppler ultrasonography with a new test for placental growth factor (PlGF) to predictfetal adverse events.Methods:Eighty-nine women with hypertensive disorders of pregnancy (24 with chronic hypertension, 17 withgestational hypertension, 12 with HELLP syndrome, 19 with preeclampsia and 17 with superimposed preeclampsia)were enrolled. A single maternal blood sample to measure free PlGF (Alere Triage) taken before 35 weeks ofpregnancy was compared to the last Doppler ultrasound measurement of fetal flow before delivery. PlGF wasclassified as normal (PlGF?100 pg/ml), low (12<PlGF<100) or very low (PlGF?12 pg/ml). A positive test for abnormalfetal flow was defined as either signs of centralisation of the fetal circulation or diastolic block or reverse flow in theumbilical artery or descending aorta; this was a criterion for delivery. Fetal outcomes were intrauterine growthrestriction and birth before 37 weeks of pregnancy.Results:In total 61/89 women had a preterm birth and 22 infants had IUGR. Of those who delivered preterm,20/20 women with abnormal fetal flow and 36/41 (87.8%) women with normal fetal flow had low or very low PlGF.Of those infants with IUGR, 22/22 had low or very low maternal PlGF and 10/22 had abnormal fetal flow.Conclusions:PlGF may provide useful information before 35th gestational week to identify fetuses requiring urgentdelivery, and those at risk of later adverse outcomes not identified by fetal flow Doppler ultrasonography.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
hypertension
placental growth factor
PlGF
IUGR
preeclampsia
Megjelenés:BMC Pregnancy and Child Birth 13 (2013), p. 1-7. -
További szerzők:Gullai Nóra Stenczer Balázs Fügedi Gergely Nagy Bálint (1956-) (molekuláris genetikus) Rigó János (1958-) (szülész-nőgyógyász)
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11.

001-es BibID:BIBFORM065297
Első szerző:Molvarec Attila (szülész-nőgyógyász)
Cím:Association between tumor necrosis factor (TNF)-α G-308A gene polymorphism and preeclampsia complicated by severe fetal growth restriction / Attila Molvarec, Ágnes Jermendy, Bálint Nagy, Margit Kovács, Tibor Várkonyi, Petronella Hupuczi, Zoltán Prohászka, János Rigó Jr.
Dátum:2008
ISSN:0009-8981
Megjegyzések:Preeclampsia and HELLP (hemolysis, elevated liver enzymes, and low platelet count) syndrome are multifactorial disorders with genetic and environmental components. Given that the tumor necrosis factor (TNF)-alpha G-308A single nucleotide polymorphism (SNP) affects TNF-alpha gene transcription and that preeclampsia and HELLP syndrome are characterized by a shift towards a Th1-type maternal immune response with increased TNF-alpha production, the aim of the current study was to investigate whether this SNP is associated with preeclampsia and HELLP syndrome in a Caucasian population from Hungary. Additionally, we aimed to examine whether TNF-alpha G-308A polymorphism can influence the risk for fetal growth restriction in preeclamptic patients, which issue none of the earlier studies dealt with.METHODS:In a case-control study, we analyzed blood samples from 140 preeclamptic patients, 69 patients with HELLP syndrome and 144 normotensive, healthy pregnant women using the polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) method. We performed also a meta-analysis with our results and those of 8 previously published studies.RESULTS:There were no significant differences in the genotype and allele frequencies of the TNF-alpha G-308A polymorphism between preeclamptic patients and normotensive, healthy pregnant women. However, the mutant (TNF2 or A) allele occurred significantly more frequently in preeclamptic patients with IUGR than in those without IUGR (18.5% versus 7.1%, p=0.003). In addition, the frequency of the mutant allele carriers was significantly higher among preeclamptic patients with IUGR compared to those without IUGR (30.6% versus 12.8%, p=0.010). The mutant allele carriers were found to have an increased risk of severe IUGR-complicated preeclampsia, which was independent of maternal age, prepregnancy BMI and primiparity (odds ratio (OR): 2.89, 95% confidence interval (CI): 1.16-7.22, p=0.023; adjusted OR: 2.78, 95% CI: 1.04-7.45, p=0.042). Nevertheless, no significant differences were detected in the genotype and allele frequencies of the TNF-alpha G-308A polymorphism between patients with HELLP syndrome and control subjects. In the meta-analysis, no association was observed between this SNP and preeclampsia (summary OR: 0.956, 95% CI: 0.693-1.319).CONCLUSIONS:Although the meta-analysis demonstrated a lack of an overall association between TNF-alpha G-308A polymorphism and preeclampsia, our results suggest a role of this SNP in the risk of severe IUGR-complicated preeclampsia. However, further studies are required with a larger sample size to confirm our findings.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Preeclampsia
Gene
Polymorphism
TNF
Megjelenés:Clinica Chimica Acta. - 392 : 1-2 (2008), p. 52-57. -
További szerzők:Jermendy Ágnes Nagy Bálint (1956-) (molekuláris genetikus) Kovács Margit (belgyógyász, Budapest) Várkonyi Tibor Hupuczi Petronella (anaesthesiológus) Prohászka Zoltán Rigó János (1958-) (szülész-nőgyógyász)
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12.

001-es BibID:BIBFORM065315
Első szerző:Molvarec Attila (szülész-nőgyógyász)
Cím:Lipid, haemostatic and inflammatory variables in relation to the estrogen receptor [alfa] (ESR1) PvuII and XbaI gene polymorphisms / Molvarec Attila, Nagy Bálint, Kovács Margit, Walentin Szilvia, Imreh Éva, Rigó János Jr., Szalay János, Füst George, Prohászka Zoltán, Karádi István
Dátum:2007
ISSN:0009-8981
Megjegyzések:Estrogen is known to affect lipoprotein metabolism, the haemostatic system and inflammatory markers. Our aim was to determine whether estrogen receptor alpha (ESR1) PvuII and XbaI gene polymorphisms can influence lipid, haemostatic and inflammatory variables in healthy Caucasian women and men of reproductive age.METHODS:58 healthy women (aged between 18 and 45 years) and 55 healthy men (aged between 21 and 45 years) of reproductive age were enrolled in our study. FSH levels, lipid (total cholesterol, triglyceride, HDL cholesterol, lipoprotein(a), apo A-I, apo B), haemostatic (prothrombin time, activated partial thromboplastin time (aPTT), thrombin time, fibrinogen, factor V, VII, VIII, protein C, protein S, antithrombin III) and inflammatory (CRP) variables were measured on autoanalyzers using commercially available kits. Serum VLDL and LDL cholesterol concentrations were calculated with the equation of Friedewald. The ESR1 PvuII and XbaI genotypes were determined with PCR-RFLP method.RESULTS:In the total group, the ESR1 XbaI GG genotype carriers had significantly higher serum lipoprotein(a) concentrations than the AA or AG genotype carriers. Serum total cholesterol concentrations were significantly higher in healthy women with the PvuII CC genotype than in those with the TT or TC genotypes, whereas healthy women with the GG genotype of the ESR1 XbaI polymorphism had significantly higher serum total cholesterol and LDL cholesterol levels compared to those with the AA or AG genotypes. No other effects of the ESR1 PvuII and XbaI polymorphisms were found on the investigated lipid, haemostatic and inflammatory variables either in the total group or in women and men separately.CONCLUSIONS:The ESR1 PvuII and XbaI gene polymorphisms seem to affect lipoprotein metabolism in healthy subjects of peak reproductive age. However, further studies are needed to determine the molecular mechanisms by which the two polymorphisms could influence serum lipid levels.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
lipid
estrogen receptor
inflammatory
gene
polymorphism
Megjelenés:Clinica Chimica Acta. - 380 : 1-2 (2007), p. 157-164. -
További szerzők:Nagy Bálint (1956-) (molekuláris genetikus) Kovács Margit (belgyógyász, Budapest) Walentin Szilvia Imreh Éva Rigó János (1958-) (szülész-nőgyógyász) Szalay János Füst György (Budapest) Prohászka Zoltán Karádi István (1952-) (belgyógyász, kardiológus)
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