CCL

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1.

001-es BibID:BIBFORM074858
Első szerző:Aalto, Yan
Cím:Distinct gene expression profiling in chronic lymphocytic leukemia with 11q23 deletion / Y. Aalto, W. El-Rifai, L. Vilpo, J. Ollila, B. Nagy, M. Vihinen, J. Vilpo, S. Knuutila
Dátum:2001
ISSN:0887-6924
Megjegyzések:Chronic lymphocytic leukemia (CLL) is a heterogeneous disease with regard to its clinical course. The limitations of the methods currently available for prognostic assessment in CLL do not allow accurate prediction of the risk of disease progression in individual patients. The recently developed cDNA array technique provides a unique opportunity to study gene expression in various malignancies. To identify new molecular markers for prognostication of CLL patients, we analyzed cDNA arrays by using hierarchical clustering and standard statistic t-test on 34 CLL patients. We found significant expression differences in 78 genes compared to the reference tonsillar B lymphocytes. A cluster of genes, LCP1, PARP, BLR1, DEK, NPM, MCL1, SLP76, STAM, HIVEP1, EVI2B, CD25, HTLF, HIVEP2, BCL2, MNDA, PBX3, EB12, TCF1, CGRP, CD14, ILB, GZMK, GPR17 and CD79B, was associated (P < 0.05) with the unfavorable 11q deletion and also with the unfavorable Binet stages B and C. We present here gene expression profiling that is associated with CLL patients with the 11q23 deletion. Many of the genes in the cluster have not previously been shown to be related to the initiation or progression of CLL. These novel findings provide fundamental information for further attempts to understand the interaction of the clustered genes in the leukomogenesis of CLL in order to better design treatments aimed at specific molecular target(s).
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
gene expression
deletion 11q23
CLL
Megjelenés:Leukemia. - 15 : 11 (2001), p. 1721-1728. -
További szerzők:El-Rifai, Wa'el Vilpo, L. Ollila, Juha Nagy Bálint (1956-) (molekuláris genetikus) Vihinen, Mauno Vilpo, Juhani Knuutila, Sakari
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2.

001-es BibID:BIBFORM065331
Első szerző:Ferrer, Anna
Cím:Different gene expression in immunoglobulin-mutated and immunoglobulin-unmutated forms of chronic lymphocytic leukemia / Anna Ferrer, Juha Ollila, Gerard Tobin, Bálint Nagy, Ulf Thunberg, Yan Aalto, Mauno Vihinen, Juhani Vilpo, Richard Rosenquist, Sakari Knuutila
Dátum:2004
ISSN:0165-4608
Megjegyzések:The mutation status of the immunoglobulin heavy chain variable regions (IgVH) has been found to be a good prognostic indicator for B-cell chronic lymphocytic leukemia (CLL) because unmutated VH genes are associated with rapid disease progression and shorter survival time. To study the differences in gene expression between the Ig-unmutated and Ig-mutated CLL subtypes, we performed gene expression profiling on 31 CLL cases and investigated the VH gene mutation status by sequencing. The array data showed that the greatest variances between the unmutated (20 cases) and the mutated (11 cases) group were in expressions of ZAP70, RAF1, PAX5, TCF1, CD44, SF1, S100A12, NUP214, DAF, GLVR1, MKK6, AF4, CX3CR1, NAFTC1, and HEX. ZAP70 was significantly more expressed in the Ig-unmutated CLL group, whereas the expression of all the other genes was higher in the Ig-mutated cases. These results corroborate a recent finding, according to which the expression of ZAP70 can predict the VH mutation status and suggest that RAF1, PAX5, and other differentially expressed genes may offer good markers for differentiating unmutated cases from mutated cases and thus serve as prognostic markers.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
gene
expression
immunoglobulin
mutated
unmutated
CLL
Megjelenés:Cancer Genetics And Cytogenetics. - 153 : 1 (2004), p. 69-72. -
További szerzők:Ollila, Juha Tobin, Gerard Nagy Bálint (1956-) (molekuláris genetikus) Thunberg, Ulf Aalto, Yan Vihinen, Mauno Vilpo, Juhani Rosenquist, Richard Knuutila, Sakari
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3.

001-es BibID:BIBFORM065346
Első szerző:Larramendy, Marcelo L.
Cím:Overexpression of translocation-associated fusion genes of FGFRI, MYC, NPMI, and DEK, but absence of the translocations in acute myeloid leukemia : a microarray analysis. / Marcelo L. Larramendy, Tarja Niini, Erkki Elonen, Bálint Nagy, Juha Ollila, Mauno Vihinen, Sakari Knuutila
Dátum:2002
ISSN:0390-6078
Megjegyzések:BACKGROUND AND OBJECTIVES:Translocation-associated gene fusions are well recognized in acute myeloid leukemia. Other molecular genetic changes are less well known. The novel cDNA technology has opened the avenue to large-scale gene expression analysis. Our aim was to perform cDNA microarray analysis of acute myeloid leukemia (AML).DESIGN AND METHODS:We performed cDNA microarray analysis using the Clontech hematology filter (containing 406 genes) on 15 patients to study gene expression profiling in AML. As reference, we used whole bone marrow from 5 healthy donors.RESULTS:Our results revealed 50 differentially expressed genes in at least 3 out of 15 patients. Twenty-two genes were upregulated (ratio > or =4), whereas 28 genes were downregulated (ratio < or =0.25). All but one of the 13 genes tested by real-time polymerase chain reaction (PCR) showed the same expression profiles. Among the overexpressed genes, several were those earlier associated with chromosomal translocations and gene fusions. These genes were FGFR1, MYC, NPM1, DEC, and BCL2. The expression of two upregulated genes, HOXA4 and CSF1R, was significantly higher in patients with a white blood cell count higher than 30 x 10(9)/L cells. In patients whose white blood cell count was higher than 100 x 10(9)/L cells, both CLC and GRN were significantly underexpressed, whereas HOXA4 and DAPK1 were overexpressed. FGFR1 and CAMLG were more frequently significantly overexpressed in patients with CD56 immunophenoytpe.INTERPRETATION AND CONCLUSIONS:Clinical and prognostic significance of differential gene expression should be studied with a larger series of patients by using other techniques, such as quantitative real-time PCR.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
microarray
AML
FGFR1
MYC
DEK
NPMI
fusion genes
Megjelenés:Haematologica. - 87 (2002), p. 569-577. -
További szerzők:Niini, Tarja Elonen, Erkki Nagy Bálint (1956-) (molekuláris genetikus) Ollila, Juha Vihinen, Mauno Knuutila, Sakari
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4.

001-es BibID:BIBFORM065329
Első szerző:Leivo, Ilmo
Cím:Characterization of gene expression in major types of salivary gland carcinomas with epithelial differentiation / Ilmo Leivo, Kowan Ja Jee, Kristiina Heikinheimo, Merja Laine, Juha Ollila, Balint Nagy, Sakari Knuutil
Dátum:2005
ISSN:0165-4608
Megjegyzések:Gene expression profiles were studied in 13 cases of salivary gland carcinoma including mucoepidermoid carcinoma (MEC), acinic cell carcinoma (ACC), and salivary duct carcinoma (SDC) using a cDNA array. A total of 162 genes were deregulated. Only 5 genes were overexpressed in all carcinomas including fibronectin 1 (FN1), tissue metalloproteinase inhibitor 1 (TIMP1), biglycan (BGN), tenascin-C (HXB), and insulin-like growth factor binding protein 5 (IGFBP5), whereas 16 genes were underexpressed. The small number of similarly deregulated genes in these carcinoma entities suggests an extensive genetic variation between them. This result agrees with the great histopathological diversity of different entities of salivary gland carcinoma. Furthermore, diversity in gene expression between the carcinoma types was identified also by hierarchical clustering. Each carcinoma entity was clustered together but MEC, SDC, and ACC were separated from each other. Significance analysis of microarrays identified 27 genes expressed differently between the groups. In MEC, overexpressed genes included those of cell proliferation (IL-6 and SFN) and cell adhesion (SEMA3F and COL6A3), whereas many underexpressed genes were related to DNA modification (NTHL1 and RBBP4). Apoptosis-related genes CASP10 and MMP11 were overexpressed in SDC, in accordance with the typical tumor necrosis seen in this entity. An intermediate filament protein of basal epithelial cells, cytokeratin 14 (KRT14) was clearly differently expressed between the 3 types of carcinoma, and can be used as an aid in their differential diagnosis. The array results were validated by RT-PCR and immunohistochemistry.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
gene
expression
salivary
carcinomas
epithelial
Megjelenés:Cancer Genetics And Cytogenetics. - 156 : 2 (2005), p. 104-113. -
További szerzők:Jee, Kowan Ja Heikinheimo, Kristiina Laine, Merja Ollila, Juha Nagy Bálint (1956-) (molekuláris genetikus) Knuutila, Sakari
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