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001-es BibID:	BIBFORM073615
Első szerző:	Zierdt, Charles H.
Cím:	Enzyme-linked immunosorbent assay for detection of serum antibody to Blastocystis hominis in symptomatic infections / Zierdt C. H., Zierdt W. S., Nagy B.
Dátum:	1995
ISSN:	0022-3395
Megjegyzések:	An enzyme-linked immunosorbent assay was devised in order to search for antibodies against Blastocystis hominis in infected humans. Reaction proteins were obtained from washed, axenic B. hominis cells, as sonicate. Sonicate was diluted to provide 17 and 34 micrograms of protein per well. Dilutions of patients' sera were applied, followed by phosphatase-conjugated goat anti-human serum and phosphatase substrate. Color was measured at 405 microns wavelength. Immunoglobulin G antibodies to high titers were found. Of 30 sera tested from 28 patients, 3 were negative at the 1/50 threshold dilution, 8 were positive at 1/50, 3 at 1/100, 2 at 1/200, 3 at 1/400, 6 at 1/800, and 5 at 1/1,600. Normal sera (42 blood bank sera) were all negative at 1/50. Each serum was subjected to multiple testing. Duplicate tests were included for each run, and runs were made from 4 to 6 for each serum. Blastocystis hominis is increasingly recognized to be a cause of human enteric disease, with symptoms often like those in giardiasis. Demonstration of strong antibody response is consistent with this view.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Blastocystis hominis ELISA antibody
Megjelenés:	Journal Of Parasitology. - 81 : 1 (1995), p. 127-129. -
További szerzők:	Zierdt, Willadene S. Nagy Bálint (1956-) (molekuláris genetikus)
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001-es BibID:	BIBFORM065371
Első szerző:	Zierdt, Charles H.
Cím:	Antibody Response to Blastocystis hominis Infections / Zierdt Charles H., Balint Nagy
Dátum:	1993
ISSN:	0003-4819
Megjegyzések:	In our study, sera were obtained from 19 patients with symptomatic B. hominis infections. Two patients provided acute-phase sera and convalescent-phase sera. An indirect fluorescent antibody test (IFA) and an enzyme-linked immunosorbent assay (ELISA) were developed to search for antibodies against B. hominis. Both the IFA and ELISA techniques demonstrated specific IgG antibody against B. hominis (Table 1). The average titer was 1/268 by IFA and 1/405 by ELISA. The average titer for 50 normal (blood bank) sera was 1/24 by IFA, and all normal sera were negative by ELISA (1/50 was the threshold dilution). Titers for the two acute-phase sera were as follows: Patient 1, 1/200 by IFA and 1/100 by ELISA; Patient 2, 1/200 by IFA and 1/200 by ELISA. Titers for convalescent-phase sera were as follows: Patient 1, 1/800 by IFA and 1/800 by ELISA; Patient 2, 1/600 by IFA and 1/600 by ELISA. The decided increase in titer for these two patients indicates recent systemic infection with B. hominis. Inflammatory response to B. hominis has been said to be part of the pathology related to this infection [4]. The immune titers reported above are much higher than those reported for giardiasis, even though both parasites are noninvasive.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok hozzászólás antibody Blastocystis hominis infestion response
Megjelenés:	Annals Of Internal Medicine. - 118 : 12 (1993), p. 985-986. -
További szerzők:	Nagy Bálint (1956-) (molekuláris genetikus)
Internet cím:	DOI Intézményi repozitóriumban (DEA) tárolt változat
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