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001-es BibID:	BIBFORM065346
Első szerző:	Larramendy, Marcelo L.
Cím:	Overexpression of translocation-associated fusion genes of FGFRI, MYC, NPMI, and DEK, but absence of the translocations in acute myeloid leukemia : a microarray analysis. / Marcelo L. Larramendy, Tarja Niini, Erkki Elonen, Bálint Nagy, Juha Ollila, Mauno Vihinen, Sakari Knuutila
Dátum:	2002
ISSN:	0390-6078
Megjegyzések:	BACKGROUND AND OBJECTIVES:Translocation-associated gene fusions are well recognized in acute myeloid leukemia. Other molecular genetic changes are less well known. The novel cDNA technology has opened the avenue to large-scale gene expression analysis. Our aim was to perform cDNA microarray analysis of acute myeloid leukemia (AML).DESIGN AND METHODS:We performed cDNA microarray analysis using the Clontech hematology filter (containing 406 genes) on 15 patients to study gene expression profiling in AML. As reference, we used whole bone marrow from 5 healthy donors.RESULTS:Our results revealed 50 differentially expressed genes in at least 3 out of 15 patients. Twenty-two genes were upregulated (ratio > or =4), whereas 28 genes were downregulated (ratio < or =0.25). All but one of the 13 genes tested by real-time polymerase chain reaction (PCR) showed the same expression profiles. Among the overexpressed genes, several were those earlier associated with chromosomal translocations and gene fusions. These genes were FGFR1, MYC, NPM1, DEC, and BCL2. The expression of two upregulated genes, HOXA4 and CSF1R, was significantly higher in patients with a white blood cell count higher than 30 x 10(9)/L cells. In patients whose white blood cell count was higher than 100 x 10(9)/L cells, both CLC and GRN were significantly underexpressed, whereas HOXA4 and DAPK1 were overexpressed. FGFR1 and CAMLG were more frequently significantly overexpressed in patients with CD56 immunophenoytpe.INTERPRETATION AND CONCLUSIONS:Clinical and prognostic significance of differential gene expression should be studied with a larger series of patients by using other techniques, such as quantitative real-time PCR.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban microarray AML FGFR1 MYC DEK NPMI fusion genes
Megjelenés:	Haematologica. - 87 (2002), p. 569-577. -
További szerzők:	Niini, Tarja Elonen, Erkki Nagy Bálint (1956-) (molekuláris genetikus) Ollila, Juha Vihinen, Mauno Knuutila, Sakari
Internet cím:	Szerző által megadott URL Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM065340
Első szerző:	Nagy Bálint (molekuláris genetikus)
Cím:	Abnormal expression of apoptosis-related genes in haematological malignancies : overexpression of MYC is poor prognostic sign in mantle cell lymphoma / Bálint Nagy, Tuija Lundán, Marcelo L. Larramendy, Yan Aalto, Ying Zhu, Tarja Niini, Henrik Edgren, Anna Ferrer, Juhani Vilpo, Erkki Elonen, Kim Vettenranta, Kaarle Franssila, Sakari Knuutila
Dátum:	2003
ISSN:	0007-1048
Megjegyzések:	The expression of apoptosis-related genes BCL2, BAX, BCL2L1, BCL2A1, MCL1, DAPK1 and MYC was studied by quantitative real-time polymerase chain reaction on total RNA samples from patients with acute lymphoblastic leukaemia (ALL, n = 16), acute myeloid leukaemia (AML, n = 27), chronic myeloid leukaemia (CML, n = 12), mantle cell lymphoma (MCL, n = 19) and chronic lymphoid leukaemia (CLL, n = 32). BCL2, BAX, BCL2A1, MCL1, DAPK1 and MYC were overexpressed in all patient groups. BCL2L1 was underexpressed in CLL and CML, but not in AML, ALL and MCL. MCL1 levels were significantly higher in CD13 and CD33-positive ALL, and in CD56-positive AML samples. BCL2, BCL2L1, BCL2A1 and MCL1 were overexpressed and DAPK1 was underexpressed in CLL samples with a 11q23 deletion. MYC overexpression was significantly associated with shorter overall survival in MCL (P < 0.01). AML patients with a normal karyotype showed a higher frequency of BCL2A1 overexpression (P < 0.001) than those with an abnormal karyotype.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban mantle cell lymphoma gene expression myc prognostic
Megjelenés:	British Journal Of Haematology. - 120 (2003), p. 434-441. -
További szerzők:	Lundán, Tuija Larramendy, Marcelo L. Aalto, Yan Zhu, Ying Niini, Tarja Edgren, Henrik Ferrer, Anna Vilpo, Juhani Elonen, Erkki Vettenranta, Kim Franssila, Kaarle Knuutila, Sakari
Internet cím:	Szerző által megadott URL Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM065344
Első szerző:	Niini, Tarja
Cím:	Expression of myeloid-specific genes in childhood acute lymphoblastic leukemia : a cDNA array study / T. Niini, K. Vettenranta, J. Hollmén, M. L. Larramendy, Y. Aalto, H. Wikman, B. Nagy, J. K. Seppänen, A. Ferrer Salvador, H. Mannila, U. M. Saarinen-Pihkala, S. Knuutila
Dátum:	2002
ISSN:	0887-6924
Megjegyzések:	Several specific cytogenetic changes are known to be associated with childhood acute lymphoblastic leukemia (ALL), and many of them are important prognostic factors for the disease. Little is known, however, about the changes in gene expression in ALL. Recently, the development of cDNA array technology has enabled the study of expression of hundreds to thousands of genes in a single experiment. We used the cDNA array method to study the gene expression profiles of 17 children with precursor-B ALL. Normal B cells from adenoids were used as reference material. We discuss the 25 genes that were most over-expressed compared to the reference. These included four genes that are normally expressed only in the myeloid lineages of the hematopoietic cells: RNASE2, GCSFR, PRTN3 and CLC. We also detected over-expression of S100A12, expressed in nerve cells but also in myeloid cells. In addition to the myeloid-specific genes, other over-expressed genes included AML1, LCP2 and FGF6. In conclusion, our study revealed novel information about gene expression in childhood ALL. The data obtained may contribute to further studies of the pathogenesis and prognosis of childhood ALL.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban expression gene myeloid ALL CDNA array
Megjelenés:	Leukemia. - 16 : 11 (2002), p. 2213-2221. -
További szerzők:	Vettenranta, Kim Hollmén, Jaakko Larramendy, Marcelo L. Aalto, Yan Wikman, Harriet Nagy Bálint (1956-) (molekuláris genetikus) Seppänen, Jouni K. Salvador, Aurora Ferrer Mannila, Heikki Saarinen-Pihkala, Ulla Maija Knuutila, Sakari
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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