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001-es BibID:BIBFORM065330
Első szerző:Kettunen, Eeva
Cím:L1CAM, INP10, P-cadherin, tPA and ITGB4 over-expression in malignant pleural mesotheliomas revealed by combined use of cDNA and tissue microarray / E. Kettunen, A. G. Nicholson, B. Nagy, H. Wikman, J. K. Seppänen, T. Stjernvall, T. Ollikainen, V. Kinnula, S. Nordling, J. Hollmén, S. Anttila, S. Knuutila
Dátum:2004
ISSN:1460-2180
Megjegyzések:Malignant pleural mesothelioma (MM) is a rare tumour with high mortality, which can exhibit various morphologies classified as epithelioid, biphasic and sarcomatoid subtypes. To investigate the molecular changes in these tumours, we studied gene expression patterns by combined use of cDNA arrays and tumour tissue microarrays (TMA). Deregulation of the expression of 588 cancer-related genes was screened in 16 MM comprising all three subtypes and compared with references, i.e. normal mesothelial cell lines and pleural mesothelium. Array data were analysed using three statistical methods; principal component analysis (PCA), permutation test and receiver operating characteristic (ROC) curves. Eleven genes were verified by real-time RT-PCR. Genes encoding two adhesion molecules [COL1A2 and integrin beta4 (ITGB4)] and a chemokine (INP10) were up-regulated in MM compared with both the cell lines and pleural mesothelium. There was a type-specific up-regulation of semaphorin E, ITGB4 and P-cadherin in epithelioid MM, matrix metalloproteinase 9 (MMP9) and tissue-type plasminogen activator (tPA) in sarcomatoid MM and neural cell adhesion molecule L1 (L1CAM) and INP10 in biphasic MM. Immunohistochemistry on TMA containing 47 MM (26 epithelioid, 15 sarcomatoid and six biphasic) was performed for five proteins, ITGB4, P-cadherin, tPA, INP10 and L1CAM. INP10 expression was increased in MM in general compared with normal mesothelium, while increased expression of P-cadherin, L1CAM and ITGB4 was more specific in MMs exhibiting an epithelioid growth pattern. The over-expression of tPA was more frequent in epithelioid MM despite higher mRNA levels in sarcomatoid and biphasic MM. We conclude that several proteins, associated with cell adhesion either directly (ITGB4, L1CAM, P-cadherin) or as a regulatory factor (INP10), are differentially expressed in MM. In particular, INP10, ITGB4 and COL1A2 were up-regulated in MM compared with both reference sample types, suggesting a relationship with development of these tumours.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
L1CAM
INP10
P-cadherin
tPA
ITGB4
overexpression
mesothelioma
Megjelenés:Carcinogenesis. - 26 : 1 (2004), p. 17-25. -
További szerzők:Nicholson, Andrew Gordon Nagy Bálint (1956-) (molekuláris genetikus) Wikman, Harriet Seppänen, Jouni K. Stjernvall, Tuula Ollikainen, Tiina Kinnula, Vuokko Nordling, Stig Hollmén, Jaakko Anttila, Sisko Knuutila, Sakari
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2.

001-es BibID:BIBFORM065334
Első szerző:Wikman, Harriet
Cím:Caveolins as tumour markers in lung cancer detected by combined use of cDNA and tissue microarrays / Harriet Wikman, Jouni K. Seppänen, Virinder K. Sarhadi, Eeva Kettunen, Kaisa Salmenkivi, Eeva Kuosma, Katri Vainio-Siukola, Balint Nagy, Antti Karjalainen, Thanos Sioris, Jarmo Salo, Jaakko Hollmén, Sakari Knuutila, Sisko Anttila
Dátum:2004
ISSN:0022-3417
Megjegyzések:To identify new potential diagnostic markers for lung cancer, the expression profiles of 37 lung tumours were analysed using cDNA arrays. Seven samples were from small-cell lung cancer (SCLC), two from large-cell neuroendocrine tumours (LCNEC), and 28 from other non-small-cell lung cancers (mainly squamous cell cancer and adenocarcinoma). Principal component analysis and the permutation test were used to detect differences in the gene expression profiles and a set of genes was found that distinguished high-grade neuroendocrine carcinomas (SCLC and LCNEC) from other lung cancers. In addition, several genes, such as caveolin-1 (CAV1) and caveolin-2 (CAV2), were constantly deregulated in all types of tumour sample, compared with normal tissue. The expression of these two genes was investigated further at the protein level on a tissue microarray containing tumours from 161 patients and normal tissues. Immunostaining for CAV1 was negative in 48% of tumours, whereas 28% of the tumours did not express CAV2. Lack of CAV1 protein expression was not caused by methylation or mutation. In stage I adenocarcinomas, CAV2 protein expression correlated with shorter survival. In conclusion, the present study was able to identify genes that have not previously been implicated in lung cancer by the combined use of two different array techniques. Some of these genes may provide novel diagnostic markers for lung cancer.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
caveolins
tumor
marker
lung cancer
cDNA
tissue
microarray
Megjelenés:Journal Of Pathology. - 203 : 1 (2004), p. 584-593. -
További szerzők:Seppänen, Jouni K. Sarhadi, Virinder K. Kettunen, Eeva Salmenkivi, Kaisa Kuosma, Eeva Vainio-Siukola, Katri Nagy Bálint (1956-) (molekuláris genetikus) Karjalainen, Antti Sioris, Thanos Salo, Jarmo Hollmén, Jaakko Knuutila, Sakari Anttila, Sisko
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
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