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001-es BibID:BIBFORM095934
Első szerző:Buglyó Gergely (genetikus)
Cím:miRNA Profiling of Hungarian Regressive Wilms' Tumor Formalin-Fixed Paraffin-Embedded (FFPE) Samples by Quantitative Real-Time Polymerase Chain Reaction (RT-PCR) / Gergely Buglyó, Zsófia Magyar, Éva Romicsné Görbe, Rita Bánusz, Monika Csóka, Tamás Micsik, Márta Mezei, Jaxi Ayman Shawky Yani, Péter Varga, Zoltán Sápi, Bálint Nagy
Dátum:2021
ISSN:1643-3750
Megjegyzések:Background: Wilms' tumor is a common renal malignancy of early childhood with a generally favorable prognosis depending upon histological subtype. It is becoming increasingly clear that differences in miRNA (microRNA) expression signature represent important clues helping us predict a tumor's response to chemotherapy. In our study, we aimed to reveal miRNAs deregulated in regressive Wilms' tumors from FFPE (formalin-fixed, paraffin-embedded) samples, also showing whether such samples are reliable miRNA sources in Wilms' tumor. Material/Methods: Samples from 8 Hungarian patients (3 males, 5 females, aged 1 to 7 years) were analyzed by qRT-PCR (quantitative real-time PCR). A PCR array was used in a pilot experiment, and selected miRNAs (miR-128-3p, miR-184, miR-194-5p, miR-203a) were studied in the rest of the samples using individual primers. Results: miR-194-5p was underexpressed in all tumor samples. miR-184 and miR-203a were underexpressed in 7 cases, the exception being a case with a high ratio of necrotic blastemal tissue. Results obtained with miR-1283p are difficult to interpret due to varying directions of expression changes. Conclusions: We conclude that a downregulation of miR-184, miR-194-5p, and miR-203a expression is observed in both regressive and blastemal tumors, but larger-scale studies are needed to confirm whether the degree of their underexpression correlates with the number of blastemal elements in a sample. In most of our FFPE samples aged up to 9 years, RNA extraction provided miRNA with quantity and quality sufficient for qRT-PCR-based analysis, emphasizing the relevance of pathological archives as miRNA sources in future studies.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
MIRN184 microRNA Human
MIRN194 microRNAHuman
MIRN203 Human
microRNA
Real-Time Polymerase Chain Reaction
Wilms Tumor
Megjelenés:Medical Science Monitor. - 27 (2021), p. e932731-1-e932731-9. -
További szerzők:Magyar Zsófia Görbe Éva Bánusz Rita Csóka Mónika Micsik Tamás Mezei Márta Yani, Jaxi Ayman Shawky Varga Péter (szülész-nőgyógyász) Sápi Zoltán Nagy Bálint (1956-) (molekuláris genetikus)
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2.

001-es BibID:BIBFORM078570
035-os BibID:(PMID)30959135 (WoS)000466942100003 (Scopus)85063935306
Első szerző:Buglyó Gergely (genetikus)
Cím:Quantitative RT-PCR-based miRNA profiling of blastemal Wilms' tumors from formalin-fixed paraffin-embedded samples / Gergely Buglyó, Zsófia Magyar, Éva Romicsné Görbe, Rita Bánusz, Monika Csóka, Tamás Micsik, Zsanett Berki, Péter Varga, Zoltán Sápi, Bálint Nagy
Dátum:2019
ISSN:0168-1656
Megjegyzések:Blastemal Wilms' tumors are associated with poor chemo-responsiveness and an adverse prognosis. Our aim was to contribute to the miRNA profiling of the disease, while demonstrating the value of archived formalin-fixed, paraffin-embedded (FFPE) samples as miRNA sources. MiRNA was extracted from tumor and normal tissues of 8 patients diagnosed with blastemal Wilms' tumor in Hungary. A quantitative real-time PCR-based protocol was used to identify miRNAs of interest and study the expression of selected miRNAs in all samples. Profiling of miRNA expression from FFPE samples turned out to be cost-effective in Wilms' tumor, as most miRNAs (including miRNA-194-5p, which was studied in all patients) showed expression alterations similar to the ones reported in the literature. MiR-184 expression was found to be lower than in previous studies, while the downregulation of miR-203a is a novel finding. MiR-184 may be downregulated in a subset of blastemal and other Wilms' tumors. A loss of miR-203a may or may not be specific to blastemal cells, but available evidence hints at its importance in the pathogenesis of Wilms' tumor. It should be considered for inclusion in future studies of miRNA expression.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Wilms' tumor
miR-184
miR-203a
miR-194-5p
miR-34c-5p
quantitative real-time PCR
Megjelenés:Journal Of Biotechnology. - 298 (2019), p. 11-15. -
További szerzők:Magyar Zsófia Görbe Éva Bánusz Rita Csóka Mónika Micsik Tamás Berki Zsanett Varga Péter (szülész-nőgyógyász) Sápi Zoltán Nagy Bálint (1956-) (molekuláris genetikus)
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DOI
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3.

001-es BibID:BIBFORM074289
Első szerző:Buglyó Gergely (genetikus)
Cím:Novel miRNA downregulations in blastemal Wilms' tumor : an FFPE-based study / Gergely Buglyó, Zsófia Magyar, Éva Romicsné Görbe, Rita Bánusz, Monika Csóka, Tamás Micsik, Zsanett Berki, Péter Varga, Zoltán Sápi, Bálint Nagy
Dátum:2018
Megjegyzések:According to the SIOP protocol, Wilms' tumor histology is assessed from the surgical sample, after pre-operative chemotherapy. The two most common subtypes are regressive and blastemal. As the presence of blastema is a sign of adverse prognosis, recent efforts have focused on revealing molecular features of blastemal Wilms' tumors that may be responsible for poor chemo-responsiveness. Characteristic miRNA-signatures can be identified from tumor tissue as well as circulating miRNA from serum. In our study, we investigated miRNA expression in 8 patients using FFPE samples from tumor and control tissues. After identifying miRNAs of interest in the first sample by aqRT-PCR Array, 4 chosen miRNAs (miR-184, miR-203a, miR-34c-5p and miR-194-5p) were studied in all samples using individual primers. Results were comparable to previous reports based on fresh frozen tissue, but also showed a deregulation of miR-203a as a novel finding, and an underexpression of miR-184 at a previously unreported magnitude that may be present in a subset of blastemal or other Wilms' tumors. As a conclusion, we may note that further investigation of miRNA expression patterns in Wilms' tumor subtypes may be warranted, and FFPE samples (not older than 7-10 years) are excellent miRNA sources to increase sample count for high-scale studies.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idézhető absztrakt
miRNA
Wilms tumor
FFPE
Megjelenés:Biomedical Papers. - 162 : Suppl. 1 (2018), p. S15. -
További szerzők:Magyar Zsófia Görbe Éva Bánusz Rita Csóka Mónika Micsik Tamás Berki Zsanett Varga Péter (szülész-nőgyógyász) Sápi Zoltán Nagy Bálint (1956-) (molekuláris genetikus)
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
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