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001-es BibID:BIBFORM108218
035-os BibID:(WOS)000823437500001 (Scopus)85134074155
Első szerző:Laczkó Levente (biológus)
Cím:The RadOrgMiner pipeline : Automated genotyping of organellar loci from RADseq data / Levente Laczkó, Sándor Jordán, Gábor Sramkó
Dátum:2022
ISSN:2041-210X
Megjegyzések:Different versions of Restriction-site-Associated DNA sequencing (RADseq) have become powerful and popular tools in molecular ecology. Although RADseq datasets are generally regarded as representative of the nuclear genome, reduced representation genomic libraries may also sample the organellar (i.e. the mitochondrial and, in the case of plants, the plastid) DNA. This cytoplasmic genetic variance provides a better understanding of evolutionary history by uncovering past hybridisation and identifying maternal or, rarely, paternal lineage due to rapid lineage sorting. We developed a pipeline that is based on existing bioinformatic tools to automatically mine and genotype organellar loci from RADseq libraries. The efficacy of our pipeline is tested on eight publicly available datasets spanning different phylogenetic levels (i.e. from family-level phylogenies to phylogeography) and RADseq methods (sdRAD, ddRAD, ezRAD, GBS) for genotyping mitochondrial and plastid loci, which were subject to phylogenetic tree reconstruction. In all cases, organellar phylogenies adequately supplemented the original studies by corroborating the large-scale picture based on RADseq or by bringing additional evidence on past or contemporary hybridisation. RADseq methods designed to achieve larger horizontal coverage (i.e. ddRAD, ezRAD) yielded longer organellar alignments, but sdRAD and GBS still provided valuable polymorphic organellar loci at no additional sequencing effort.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Methods in Ecology and Evolution. - 13 : 9 (2022), p. 1962-1975. -
További szerzők:Jordán Sándor (1992-) Sramkó Gábor (1981-) (biológus)
Pályázati támogatás:OTKA FK137962
OTKA
ÚNKP-20-4-I-DE-290
egyéb
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DOI
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2.

001-es BibID:BIBFORM089598
035-os BibID:(WOS)000507139300001 (Scopus)85077993919
Első szerző:Lovas-Kiss Ádám (biológus, botanikus)
Cím:Seed mass, hardness, and phylogeny explain the potential for endozoochory by granivorous waterbirds / Lovas-Kiss Ádám, Vincze Orsolya, Kleyheeg Erik, Sramkó Gábor, Laczkó Levente, Fekete Réka, Molnár V. Attila, Green J. Andy
Dátum:2020
ISSN:2045-7758
Megjegyzések:Field studies have shown that waterbirds, especially members of the Anatidae family, are major vectors of dispersal by endozoochory for a broad range of plants lacking a fleshy fruit, yet whose propagules can survive gut passage. Widely adopted dispersal syndromes ignore this dispersal mechanism, and we currently have little understanding of what traits determine the potential of angiosperms for endozoochory by waterbirds. Results from previous experimental studies have been inconsistent as to how seed traits affect seed survival and retention time in the gut and have failed to control for the influence of plant phylogeny. Using 13 angiosperm species from aquatic and terrestrial habitats representing nine families, we examined the effects of seed size, shape, and hardness on the proportion of seeds surviving gut passage through mallards (Anas platyrhynchos) and their retention time within the gut. We compiled a molecular phylogeny for these species and controlled for the nonindependence of taxa due to common descent in our analyses. Intact seeds from all 13 species were egested, but seed survival was strongly determined by phylogeny and by partial effects of seed mass and hardness (wet load): species with seeds harder than expected from their size, and smaller than expected from their loading, had greater survival. Once phylogeny was controlled for, a positive partial effect of seed roundness on seed survival was also revealed. Species with seeds harder than expected from their size had a longer mean retention time, a result retained after controlling for phylogeny. Our study is the first to demonstrate that seed shape and phylogeny are important predictors of seed survival in the avian gut. Our results demonstrate that the importance of controlling simultaneously for multiple traits and relating single traits (e.g., seed size) alone to seed survival or retention time is not a reliable way to detect important patterns, especially when phylogenetic effects are ignored.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Anas platyrhynchos
endozoochory
phylogeny
retention time
seed dispersal
seed traits
Megjelenés:Ecology and Evolution. - 10 : 3 (2020), p. 1413-1424. -
További szerzők:Vincze Orsolya (1988-) (biológus) Kleyheeg Erik Sramkó Gábor (1981-) (biológus) Laczkó Levente (1992-) (biológus) Fekete Réka (1993-) (biológus) Molnár V. Attila (1969-) (biológus, botanikus) Green, Andy J.
Pályázati támogatás:OTKA-108992
OTKA
ÚNKP-19-4-DE-172
Egyéb
ÚNKP-19-4-DE-538
Egyéb
ÚNKP-18-3-I-DE-355
Egyéb
NKFIH-FK-127939
Egyéb
NKFIH-KH-129520
Egyéb
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
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3.

001-es BibID:BIBFORM105609
035-os BibID:(WoS)000824385400001 (Scopus)85135091844
Első szerző:Miklós Máté (biológus)
Cím:Seasonal variation of genotypes and reproductive plasticity in a facultative clonal freshwater invertebrate animal (Hydra oligactis) living in a temperate lake / Máté Miklós, Levente Laczkó, Gábor Sramkó, Zoltán Barta, Jácint Tökölyi
Dátum:2022
ISSN:2045-7758
Megjegyzések:Facultative sexual organisms combine sexual and asexual reproduction within a single life cycle, often switching between reproductive modes depending on environmental conditions. These organisms frequently inhabit variable seasonal environments, where favorable periods alternate with unfavorable periods, generating temporally varying selection pressures that strongly influence life history decisions and hence population dynamics. Due to the rapidly accelerating changes in our global environment today, understanding the population dynamics and genetic changes in facultative sexual populations inhabiting seasonal environments is critical to assess and prepare for additional challenges that will affect such ecosystems. In this study, we aimed at obtaining insights into the seasonal population dynamics of the facultative sexual freshwater cnidarian Hydra oligactis through a combination of restriction siteassociated sequencing (RAD-Seq) genotyping and the collection of phenotypic data on the reproductive strategy of field-collected hydra strains in a standard laboratory environment. We reliably detected 42 MlGs from the 121 collected hydra strains. Most of MLGs (N =?35, 83.3%) were detected in only one season. Five MLGs (11.9%) were detected in two seasons, one (2.4%) in three seasons and one (2.4%) in all four seasons. We found no significant genetic change during the 2?years in the study population. Clone lines were detected between seasons and even years, suggesting that clonal lineages can persist for a long time in a natural population. We also found that distinct genotypes differ in sexual reproduction frequency, but these differences did not affect whether genotypes reappeared across samplings. Our study provides key insights into the biology of natural hydra populations, while also contributing to understanding the population biology of facultative sexual species inhabiting freshwater ecosystems.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
clonal reproduction
facultative sexuality
phenotypic plasticity
population genomics
RAD-seq
Megjelenés:Ecology and Evolution. - 12 : 7 (2022), p. 1-13. -
További szerzők:Laczkó Levente (1992-) (biológus) Sramkó Gábor (1981-) (biológus) Barta Zoltán (1967-) (biológus, zoológus) Tökölyi Jácint (1984-) (biológus)
Pályázati támogatás:NKFIH-FK 124164
Egyéb
TKP2020-IKA-04
Egyéb
ÚNKP21-4-I
OTKA
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:
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