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001-es BibID:BIBFORM110666
035-os BibID:(scopus)85151843463
Első szerző:Bonroy, Carolien
Cím:Detection of antinuclear antibodies : recommendations from EFLM, EASI and ICAP / Bonroy Carolien, Vercammen Martine, Fierz Walter, Andrade Luis E. C., Van Hoovels Lieve, Infantino Maria, Fritzler Marvin J., Bogdanos Dimitrios, Kozmar Ana, Nespola Benoit, Broeders Sylvia, Patel Dina, Herold Manfred, Zheng Bing, Chan Eric Y. T., Uibo Raivo, Haapala Anna-Maija, Musset Lucile, Sack Ulrich, Nagy Gabor, Sundic Tatjana, Fischer Katarzyna, Rego de Sousa Maria-José, Vargas Maria Luisa, Eriksson Catharina, Heijnen Ingmar, García-De La Torre Ignacio, Carballo Orlando Gabriel, Satoh Minoru, Kim Kyeong-Hee, Chan Edward K. L., Damoiseaux Jan, Lopez-Hoyos Marcos, Bossuyt Xavier, European Federation of Laboratory Medicine (EFLM) Working Group "Autoimmunity Testing", European Autoimmune Standardization Initiative (EASI), International Consensus on Antinuclear Antibody Patterns (ICAP)
Dátum:2023
ISSN:1434-6621
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Clinical Chemistry And Laboratory Medicine. - 61 : 9 (2023), p. 1167-1198. -
További szerzők:Vercammen, Martine Fierz, Walter Andrade, Luis E. C. Van Hoovels, Lieve Infantino, Maria Fritzler, Marvin J. Bogdanos, Dimitrios P. Kozmar, Ana Nespola, Benoit Broeders, Sylvia Patel, Dina Herold, Manfred Zheng, Bing Chan, Eric Y. T. Uibo, Raivo Haapala, Anna-Maija Musset, Lucile Sack, Ulrich Nagy Gábor (1974-) (laboratóriumi szakorvos, laboratóriumi hematológus és immunológus) Sundic, Tatjana Fischer, Katarzyna Rego Sousa, Maria José Vargas, Maria Luisa Eriksson, Catharina Heijnen, Ingmar A. García-De La Torre, Ignacio Carballo, Orlando Gabriel Satoh, Minoru Kim, Kyeong-Hee Chan, Edward K. L. Damoiseaux, Jan Lopez-Hoyos, Marcos Bossuyt, Xavier European Federation of Laboratory Medicine (EFLM) Working Group "Autoimmunity Testing" European Autoimmunity Standardisation Initiative International Consensus on Antinuclear Antibody Patterns (ICAP)
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2.

001-es BibID:BIBFORM075566
Első szerző:Nagy Gábor (laboratóriumi szakorvos, laboratóriumi hematológus és immunológus)
Cím:Monitoring of drug level and anti-drug antibody production during vedolizumab therapy in patients with inflammatory bowel disease / G. Nagy, É. Török, K. Palatka, Z. Kébel, P. Antal-Szalmás
Dátum:2018
Megjegyzések:Integrin ?4?7 is expressed on gut-specific lymphocytes and plays a pivotal role in their migration to the intestine. Vedolizumab (VDZ, trade name Entyvio), a humanized monoclonal IgG1 antibody to the ?4?7 integrin blocks their adhesion to the gut vascular endothelium. In 2014 Entyvio was approved for patients with ulcerative colitis (UC) and Crohn's disease (CD) in Hungary. Our aim was to find and evaluate laboratory tests capable of measuring vedolizumab and anti-vedolizumab antibody (AVA) levels. After overviewing the available methods, LISA-TRACKER Duo Vedolizumab ELISA kit (ref: LTV 005, TheraDiag, Croissy-Beaubourg, France) was chosen. Seventeen samples of 15 patients (9 UC/6 CD) were analyzed. Mean VDZ levels were 18.2 ?g/mL and 7.4 ?g/mL in patients with UC and CD, respectively (p=0.242). Drug levels were significantly higher in patients on concomitant immune modulating therapy (16.9 ?g/mL vs 3.9 ?g/mL, p=0.033). There was no significant correlation between drug concentrations and CRP or disease activity scores. Anti-vedolizumab antibody was not detected in any of the patients (0/15) in accordance with the approximately 4% AVA positivity reported in vedolizumab immunogenicity studies (p=0.430). Five patients had drug levels under the measuring range of the test (<2 ?g/mL) suggesting the possibility of having low affinity anti-drug antibodies not detected by the bridging ELISA method used. In conclusion, LISA-TRACKER Duo Vedolizumab ELISA kit seems to be appropriate to monitor drug and anti-drug antibody levels in patients with inflammatory bowel disease. However, insensitivity of the ELISA methods for detecting low affinity anti-drug antibody may limit its use to determine immunogenicity of vedolizumab.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idézhető absztrakt
Megjelenés:Clinical Chemistry and Laboratory Medicine. - 56 : 9 (2018), p. eA162. -
További szerzők:Török Éva Palatka Károly (1961-) (belgyógyász, gasztroenterológus) Kébel Z. Antal-Szalmás Péter (1968-) (laboratóriumi szakorvos)
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3.

001-es BibID:BIBFORM075565
Első szerző:Nagy Gábor (laboratóriumi szakorvos, laboratóriumi hematológus és immunológus)
Cím:Diagnostic algorithm for antinuclear antibody testing : clinical and financial considerations / G. Nagy, I. Csípő, E. Gyimesi, J. Tóth, S. Demeter, P. Antal-Szalmás
Dátum:2018
Megjegyzések:The diagnostics of autoimmune rheumatic diseases strongly depends on laboratory tests, dominantly on autoantibody determinations. Several techniques and assay types are available for evaluation of the autoantibody profile of these patients. The most widely used indirect immunofluorescence (IIF) assay on HEp-2 cells can identify dozens of antinuclear (ANA) and anti-cytoplasmic autoantibodies, while ELISA-s or immunoblots utilizing mixed or single antigens can identify the exact specificity of them. Since the application (replacement and sequential order) of these tests is rather ambiguous we developed an algorithm for the most frequently used antinuclear and anti-cytoplasmic autoantibodies and evaluated clinical and financial efficacy of this novel system. The number and results of autoantibody determinations (ANA HEp-2 IIF, anti-dsDNA and anti-ENA tests) between January and June, 2017 were collected from our laboratory information system (GLIMS). The theoretical number of autoantibody tests was recalculated along the following rule: anti-dsDNA and anti-ENA tests were performed only if ANA HEp-2 IIF was positive. Separate analyses were performed taking into account the ANA HEp-2 IIF patterns and titers, too. Our results showed that the ANA HEp-2 IIF guided selection of anti-dsDNA and anti-ENA ELISAs could reduce the number of anti-dsDNA and anti-ENA tests by 48% and 56%, sparing about 7.1M HUF per year. The rate of ANA HEp-2 IIF negative but anti-dsDNA positive cases was 1.8%, meaning 64 samples, from which 16 patients (0.45%) had significantly higher (>2x URL) anti-dsDNA value. The rate of ANA HEp-2 IIF negative but anti-ENA positive samples was 1.1% (56 cases). 14 and 19 of these patients showed only anti-SS-A or anti-Jo-1 positivity, known to be weakly reactive on HEp-2 cells. The described sequential application of ANA HEp-2 IIF assay, anti-dsDNA and anti-ENA ELISAs provided high clinical efficacy and proved to be cost-effective.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idézhető absztrakt
Megjelenés:Clinical Chemistry and Laboratory Medicine. - 56 : 9 (2018), p. eA139-eA140. -
További szerzők:Csípő István (1953-) (vegyész) Gyimesi Edit (1957-) (klinikai biokémikus, vegyész) Tóth Judit (1978-) (laboratóriumi szakorvos) Demeter S. Antal-Szalmás Péter (1968-) (laboratóriumi szakorvos)
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4.

001-es BibID:BIBFORM071357
Első szerző:Nagy Gábor (laboratóriumi szakorvos, laboratóriumi hematológus és immunológus)
Cím:The role of automated microscopes and computer aided pattern recognition in autoantibody detection by indirect / G. Nagy, I. Csípő, J. Kappelmayer, P. Antal-Szalmás
Dátum:2016
Megjegyzések:The role of automated microscopes and computer aided pattern recognition in autoantibody detection by indirectimmunofluorescence assaysG. Nagy, I. Csípő, J. Kappelmayer, P. Antal-SzalmásDepartment of Laboratory Medicine, Faculty of Medicine, University of Debrecen, Debrecen, HungaryIndirect immunofluorescene assays (IFA) are versatile and sensitive solid phase tests for detecting autoantibodies needed to confirm the diagnosisof autoimmune diseases. Utilization of cells or tissue sections as the antigen source makes these assays capable of detecting antibodiesto delicate epitopes masked in other tests such as enzyme linked immunosorbent assay or immunoblot. However, conventional microscopicevaluation of the fluorescent patterns is time consuming and prone to transcription error.In our work we compared four automated fluorescence microscopes (Helios-Aesku Diagnostics, NovaView-Werfen Group, Europattern-Euroimmun, Image Navigator-Immunoconcept) regarding the number of available antigen substrates, specifications of the image capture andanalysis system, extent of the automation, patient safety and impact on the daily routine workflow.All four systems are able to detect antinuclear (ANA) and anti-cytoplasmic antibodies on HEp-2 epithelial cells (positive/negative discriminationonly) while Europattern and NovaView also help with the recognition of the most frequent ANA patterns. The sensitivity ofANA positive/negative discrimination is rather similar between the systems (around 95%), while the specificity varies between 85 to 95%.The capability for detection of anti-neutrophil cytoplasmic antibodies (ANCA) and image capture from other substrates like rat liver-kidneystomach(LKS) shows variability between the different analyzers. Similarly, the throughput, titer-estimation and physical parameters differsignificantly.Automation of the indirect immunofluorescence autoantibody testing is beneficial. In addition to slide processing, several fluorescencemicroscopes are available now which are able to digitize and archive IFA images. Their image analysis software helps to evaluate samples,making the indirect immunofluorescence method less laborious and error-prone.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idézhető absztrakt
Megjelenés:Clinical Chemistry and Laboratory Medicine 54 : 10 (2016), p. 202. -
További szerzők:Csípő István (1953-) (vegyész) Kappelmayer János (1960-) (absztraktok) Antal-Szalmás Péter (1968-) (laboratóriumi szakorvos)
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