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001-es BibID:BIBFORM071241
Első szerző:Pataki Emese (molekuláris biológus)
Cím:Interspecific complementation analysis between Schizosaccharomyces pombe and Candida albicans / Emese Pataki, Mátyás Sipiczki, Ida Miklós
Dátum:2015
ISSN:1217-8950 1588-2640
Megjegyzések:Candida albicans is the most common dimorphic human fungal pathogen. The pathogenesis of itsinfection is poorly understood. A recent study showed that Cas5 played an important role in thepathogenesis of Candida albicans infections and it revealed that Cas5 is required for expression ofcaspofungin-inducible genes, many of which had connection to cell wall integrity. Since defense ofthe host immune system based on the recognition of conserved molecular patterns of the fungal cellwall, deficiencies in the cell wall structure might promote the recognition and elimination ofCandida albicans by host immune cells. Cas5+ encodes a zink-finger transcription factor that ispartially homologous with rsv1+ gene of Schizosaccharomyces group. Based on the sequencesimilarity it is presumable, that rsv1 and Cas5 have the same function. To prove their functionalhomology, we started the interspecific complementation analysis. Namely, we amplified the Cas5+gene by PCR and cloned into S. pombe nmt promoter containing overexpression vector. This DNAconstruction was transformed into the S. pombe rsv1 mutant strain. The characterization and thecaspofungin sensitivity of the transformants are in progress.
Tárgyszavak:Természettudományok Biológiai tudományok idézhető absztrakt
Megjelenés:Acta Microbiologica et Immunologica Hungarica 62 : Suppl. (2015), p. 197. -
További szerzők:Sipiczki Mátyás (1948-) (biológus) Miklós Ida (1962-) (biológus)
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2.

001-es BibID:BIBFORM071242
Első szerző:Pataki Emese (molekuláris biológus)
Cím:fhl1 controls transcription of the nitrogen starvation induced genes in cooperation with TORC1 in Schizosaccharomyces pombe / Emese Pataki, Ronit Weisman, Mátyás Sipiczki, Ida Miklós
Dátum:2015
ISSN:1217-8950 1588-2640
Megjegyzések:One of the most important regulators of cellular processes is the protein kinase TOR (Target ofRapamycin), which is conserved from yeast to humans. TOR is a protein kinase that was isolated asthe target of the immunosuppressive and anticancer drug rapamycin. It regulates the response toenvironmental changes and cellular energy status, cell growth, cell proliferation, cell survival,protein synthesis, and transcription. In the fission yeast, Schizosaccharomyces pombe there are twoTOR homologs. Tor1 is part of a protein complex known as TORC2, while Tor2 is part of a proteincomplex known as TORC1. These complexes were found to regulate oppositely the response tonutrient depletion, in particular the response to nitrogen starvation. TORC1 is essential and playsmajor role in the control of cellular growth, while TORC2 regulates cell survival under stressconditions. Here we demonstrate that overexpression of fhl1+ gene suppresses the mRNA level ofthe nitrogen starvation response genes: mei2, ste4, ste11 and decreases the mating efficiency of tor2-ts mutant. Moreover overexpression of fhl1+ modifies the stress response of tor2-ts mutant, amongothers in the presence of rapamycin.Based on these findings we can conclude that fhl1 plays a role in the TOR pathway and tor2controls the response to nitrogen starvation via fhl1 transcription factor.
Tárgyszavak:Természettudományok Biológiai tudományok idézhető absztrakt
Megjelenés:Acta Microbiologica et Immunologica Hungarica 62 : Suppl. (2015), p. 197-197. -
További szerzők:Weisman, Ronit Sipiczki Mátyás (1948-) (biológus) Miklós Ida (1962-) (biológus)
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3.

001-es BibID:BIBFORM071243
Első szerző:Pataki Emese (molekuláris biológus)
Cím:Microarray analysis of a forkhead transcription factor of Schizosaccharomyces pombe / Emese Pataki, Ida Miklos, Matthias Sipiczki
Dátum:2013
ISSN:1217-8950 1588-2640
Megjegyzések:The forkhead proteins are a family of the transcription factors that play important roles in regulatingthe expression of genes, involved in cell growth, cell division, sexual differentiation or in embryonicdevelopment. [1] [2] The forkhead family of proteins contain a highly conserved Fork Head domainthat are widespread in eukaryotes, found throughout yeast and human.To understand the process of regulating of forkhead transcription factors, researchers use modelorganisms, such as Saccharomyces cerevisiae or the fission yeast Schizosaccharomyces pombe.Earlier we started to characterize the Fhl1 forkhead gene of Sch. pombe. It revealed that deletion ofthis gene lead to slow cell growth, delayed G2-to-M transition and modify the response ofenvironmental stresses[1]. Our aim was to carry on these experiments and gain more informationabout function of the Sch. pombe Fhl1 gene. A microarray analysis of the Fhl1 deleted strain wascarried out. Our results suggest that Fhl1 gene display a remarkable function in meiosis as regulatesthe ste11 gene. It also influences the transport mechanisms and kinases. Its target genes can befound at all chromosomes. Both down- and up regulated genes appeared among its targets.
Tárgyszavak:Természettudományok Biológiai tudományok idézhető absztrakt
Megjelenés:Acta Microbiologica et Immunologica Hungarica 60 : Suppl. (2013), p. 212-213. -
További szerzők:Miklós Ida (1962-) (biológus) Sipiczki Mátyás (1948-) (biológus)
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4.

001-es BibID:BIBFORM071244
Első szerző:Pataki Emese (molekuláris biológus)
Cím:rsv1 gene has functional homology in the Schizosaccharomyces species / Emese Pataki, Ida Miklos, Matthias Sipiczki
Dátum:2013
ISSN:1217-8950 1588-2640
Megjegyzések:Transcriptional regulators are involved in the regulation of gene expression. They are usually boundto the special sites of DNA sequence and hereby allow for a sophisticated response to multipleconditions in the environment. One of the transcriptional regulatory proteins is the rsv1, which ispartly characterised in Sch. pombe. It is a negative regulator of cellular carbohydrate metabolicprocess. It encodes a zinc finger protein, which is required for cell viability in stationary phaseinduced by glucose exhaustion. Cells lacking rsv1+ are viable and the gene is regulated by pka1. [1]The four fission yeast species form an early-branching clade among the Ascomycete. One of them isthe Sch. pombe, which is a well-known model organism. Its relatives, such as Sch. japonicus, Sch.octosporus or Sch. cryophilus can also be attractive models of the genetic studies. We wanted to getinformation about the role of Sch. octosporus rsv1 gene. As there is not rsv1 mutant strain at thismoment in this species, its characterisation is impossible. Therefore, we used the quantification ofsequence similarity and interspecific complementation analysis to prove the function of Sch.octosporus rsv1 gene. The BLASTp analysis suggested, that SOCG_03341 gene must be the rsv1gene. However, sequence-based functional predictions are reliable, not all homologs have the samefunction. Therefore, we cloned the Sch. octosporus rsv1 gene into a Sch. pombe vector, and thisDNA construction was transformed into the Sch. pombe rsv1 mutant strain.The characterisation of the transformants proved the functional homology between the Sch. pombersv1 and Sch. octosporus SOCG_03341 gene.
Tárgyszavak:Természettudományok Biológiai tudományok idézhető absztrakt
Megjelenés:Acta Microbiologica et Immunologica Hungarica 60 : Suppl. (2013), p. 213. -
További szerzők:Miklós Ida (1962-) (biológus) Sipiczki Mátyás (1948-) (biológus)
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DOI
Intézményi repozitóriumban (DEA) tárolt változat
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