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001-es BibID:BIBFORM070925
Első szerző:Nemesházi Edina
Cím:Natural and anthropogenic influences on the population structure of white-tailed eagles in the Carpathian Basin and central Europe / Nemesházi Edina, Kövér Szilvia, Zachos Frank E., Horváth Zoltán, Tihanyi Gábor, Mórocz Attila, Mikuska Tibor, Hám István, Literák Ivan, Ponnikas Suvi, Mizera Tadeusz, Szabó Krisztián
Dátum:2016
ISSN:0908-8857
Megjegyzések:Europeanpopulations of the white-tailed eagle Haliaeetus albicilla suffered a drastic decline during the 20th century. Inmany countries, only a few dozen breeding pairs survived or the species disappeared completely. By today, the populationshave recovered, naturally or through restocking (e.g. in Scotland or the Czech Republic). In the Carpathian Basin, whichis now a stronghold in southern Europe for the species in the southern part of the distribution range with more than 500breeding pairs, only about 50 pairs survived the bottleneck. This region provides important wintering places for individualsarriving from different regions of Eurasia. In the present study, we investigated 249 DNA samples from several Europeancountries, using 11 microsatellites and mitochondrial control region sequences (499 bp), to answer two main questions:1) did the Carpathian Basin population recover through local population expansion or is there a significant gene flowfrom more distant populations as well? 2) Does the Czech population show signs in its genetic structure of the restockingwith birds of unknown origin? Our microsatellite data yielded three genetically separate lineages within Europe: northern,central and southern, the latter being present exclusively in the Carpathian Basin. Sequencing of mitochondrial DNArevealed that there is one haplotype (B12) which is not only exclusive to the Carpathian Basin but it is frequent in thispopulation. Our results suggest that in accordance with the presumably philopatric behaviour of the species, recovery ofthe Carpathian Basin population was mainly local, but some of the wintering birds coming from the northern and centralpopulations contributed to its genetic composition as well. We detected considerably higher proportions of northern birdswithin the Czech Republic compared to the neighbouring areas, making it likely that parents of the reintroduced birdscame from northern populations.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
population structure
white-tailed eagle
Carpathian Basin
Megjelenés:Journal Of Avian Biology 47 : 6 (2016), p. 795-805. -
További szerzők:Kövér Szilvia Zachos, Frank E. Horváth Zoltán Tihanyi Gábor Mórocz Attila Mikuska Tibor Hám István Literák Ivan Ponnikas, Suvi Mizera, Tadeusz Szabó Krisztián (1975-) (zoológus)
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001-es BibID:BIBFORM070932
Első szerző:Vili Nóra
Cím:Factors affecting DNA quality in feathers used for non-invasive sampling / Vili Nóra, Nemesházi Edina, Kovács Szilvia, Horváth Márton, Kalmár Lajos, Szabó Krisztián
Dátum:2013
ISSN:2193-7192 2193-7206
Megjegyzések:The development of genetic methods broadens the scope of non-invasive sample types. Large shed feathers are good material for genetic analyses, since they are easy to collect and a single feather can provide a sufficient amount of DNA for PCR-based methods. Previous studies have demonstrated that feather quality and type affect the DNA quality extracted from the feather tips. Besides the tip, the superior umbilicus part of the shaft is also proposed as an appropriate source of DNA. In our study, we examined whether some feather parameters (physical condition, type and size) and storage time affect amplification success of DNA extracted from the superior umbilicus of shed Eastern Imperial Eagle (Aquila heliaca) feathers. We also tested the effects of sunlight, temperature and humidity on DNA extracted from Domestic Goose (Anser anser domesticus) feathers with amplification of fragments of various sizes, modelling the environmental conditions of the moulting season. While good quality feathers usually provided sufficient DNA, the usability of the DNA extracted from moderate quality feathers were affected by feather type. DNA quality was influenced in order of importance by humidity, direct sunlight and heat. Our findings support the usability of DNA samples derived from the superior umbilicus of shed feathers, and help to schedule field work by careful consideration of our results about feather quality and environmental factors.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Aquila heliaca
Anser anser
Shed feathers
Non-invasive sampling
DNA quality
Megjelenés:Journal of Ornithology. - 154 : 2 (2013), p. 587-595. -
További szerzők:Nemesházi Edina Kovács Szilvia Horváth Márton Kalmár Lajos Szabó Krisztián (1975-) (zoológus)
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