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001-es BibID:BIBFORM071070
035-os BibID:(WOS)000410115800448
Első szerző:Mihály Johanna (biológus, vegyész)
Cím:Assessment of the anti-inflammatory effects of fluorinated semi-synthetic phytocannabinoids in human in vitro inflammatory keratinocyte model systems / Mihaly J., Miltner N., Tubak V., Mechoulam R., Russo E., Biro T.
Dátum:2017
ISSN:0022-202X
Megjegyzések:It is common wisdom in pharmacology that fluorination can significantly increase the efficacy of the active components in pharmaceuticals ? actually, ca. 30% of the best-selling drugs worldwide contain fluorinated compounds. The laboratory of Prof. Mechoulam has recently synthesized a series of fluorinated derivatives of cannabidiol (CBD), the major non-psychoactive component of the plant Cannabis sativa. The goal of the current study was to assess the potential cutaneous anti-inflammatory actions of these compounds (F-CBDs).Effects of CBD and F-CBDs (HUF-101, HUF-103 and HU-559a) were investigated in seven, previously established in vitro human epidermal keratinocyte models (employing the immortalized HaCaT and HPV-KER keratinocyte cell lines) which mimic, as closely as possible, various human inflammatory skin conditions and diseases (e.g. microbial, UVB-induced, allergic, contact, and atopic dermatitis). Gene expression and protein release were assessed by RT-qPCR and ELISA, respectively. As expected, expressions of certain pro-inflammatory cytokines (e.g. interleukin [IL]-1?, IL-1?, IL-6 and IL-8) were significantly down-regulated upon the administration of CBD and F-CBDs in most models. Of great importance, however, all F-CBDs exhibited significantly higher efficacies in comparison to the non-fluorinated counterpart CBD, with the rank order of efficacy in the in vitro human epidermal keratinocyte models being HUF103 > HU559a > HU101.Our study provides the first evidence that F-CBDs exert remarkable anti-inflammatory actions on human epidermal keratinocytes. These intriguing data invite further pre-clinical and clinical studies to exploit the therapeutic potential of certain F-CBDs in a various cutaneous inflammatory conditions.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idézhető absztrakt
folyóiratcikk
anti-inflammatory
fluorinated phytocannabinoids
Megjelenés:Journal Of Investigative Dermatology. - 137 : 10 (2017), p. S271. -
További szerzők:Miltner Noémi (1990-) (molekuláris biológus) Tubak Vilmos Mechoulam, Raphael Russo, Ethan Bíró Tamás (1968-) (élettanász) (absztraktok)
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DOI
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2.

001-es BibID:BIBFORM077349
Első szerző:Miltner Noémi (molekuláris biológus)
Cím:Assessment of the anti-inflammatory effects of fluorinated semi-synthetic phytocannabinoids in human in vitro inflammatory keratinocyte model systems / Noémi Miltner, Johanna Mihály, Vilmos Tubak, Raphael Mechoulam, Ethan Russo, Tamás Bíró
Dátum:2017
ISSN:2061-0203
Tárgyszavak:Orvostudományok Elméleti orvostudományok idézhető absztrakt
Megjelenés:Immunológiai Szemle. - 3 : 3 (2017), p. 42. -
További szerzők:Mihály Johanna (1982-) (biológus, vegyész) Tubak Vilmos Mechoulam, Raphael Russo, Ethan Bíró Tamás (1968-) (élettanász)
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
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3.

001-es BibID:BIBFORM077347
Első szerző:Miltner Noémi (molekuláris biológus)
Cím:Investigation of anti-inflammatory effect of β-caryophyllene in human in vitro inflammatory keratinocyte model systems / Miltner Noémi, Mihály Johanna, Tubak Vilmos, Mechoulam Raphael, Bíró Tamás
Dátum:2018
ISSN:0006-7768 2064-261X
Tárgyszavak:Orvostudományok Elméleti orvostudományok idézhető absztrakt
Megjelenés:Bőrgyógyászati és Venerológiai Szemle. - 94 : 6 (2018), p. 309. -
További szerzők:Mihály Johanna (1982-) (biológus, vegyész) Tubak Vilmos Mechoulam, Raphael Bíró Tamás (1968-) (élettanász)
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
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4.

001-es BibID:BIBFORM074173
035-os BibID:(WOS)000431498600282
Első szerző:Miltner Noémi (molekuláris biológus)
Cím:Assessment of the anti-inflammatory effects of cannabidiol and its fluorinated derivative in in vitro and in vivo models of atopic dermatitis / Miltner Noémi, Béke Gabriella, Angyal Ágnes, Kemény Ágnes, Pintér Erika, Helyes Zsuzsanna, Bíró Tamás, Mihály Johanna
Dátum:2018
ISSN:0022-202X 1523-1747
Megjegyzések:Noémi Miltner1, Gabriella Béke1, Ágnes Angyal1, Ágnes Kemény2, Erika Pintér2, Zsuzsanna Helyes2, Tamás Bíró1,3, Johanna Mihály1Assessment of the anti-inflammatory effects of cannabidiol and its fluorinated derivative in in vitro and in vivo models of atopic dermatitis1Department of Immunology, Faculty of Medicine, University of Debrecen, Hungary2Department of Pharmacology and Pharmacotherapy, Faculty of Medicine, University of Pécs, Hungary3Phytecs Inc., Los Angeles, CA, USAIt is common wisdom in pharmacology that fluorination can significantly increase the efficacy of the active components in pharmaceuticals ? actually, ca. 30% of the best-selling drugs worldwide contain fluorinated compounds. The aim of the current study was to assess the potential anti-inflammatory effects of cannabidiol (CBD), the major non-psychoactive component of the pant Cannabis sativa, and its fluorinated derivative (HUF-101) in various experimental systems modeling atopic dermatitis (AD). For the in vitro AD model, human epidermal keratinocytes were challenged with the combination of Staphylococcus aureus enterotoxin B (SEB) and thymic stromal lymphopoietin (TSLP), and expressions of certain marker molecules were assessed by RT-qPCR and ELISA. For the in vivo model, mice were sensitized with 2% oxazolone (OXA) before elicitation. Test compounds were applied topically (1 and 10 ?M) after inducing skin inflammation and edema formation (in the ears) was measured with an engineer's micrometer.In the in vitro model, expressions of certain pro-inflammatory cytokines (e.g. interleukin [IL]-1?, IL-1?, IL-6 and IL-8) were significantly down-regulated upon the administration of CBD and HUF-101. Of great importance, however, HUF-101 exhibited significantly higher potency in comparison to CBD. In the in vivo model, topical application of 1 ?M CBD significantly reduced the OXA-induced ear edema; however, 10 ?M CBD exerted insignificant effect. In contrast, HUF-101 attenuated OXA-induced edema formation at both concentrations. Intriguingly, similar to the in vitro conditions, the anti-inflammatory potency of HUF-101 was significantly greater than that of CBD. Our study provides the first evidence that CBD and its fluorinated derivative exert significant anti-inflammatory actions in models of AD. These intriguing data invite further pre-clinical and clinical studies to exploit the therapeutic potential of certain CBD derivatives in cutaneous inflammatory conditions.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idézhető absztrakt
folyóiratcikk
Megjelenés:Journal of Investigative Dermatology. - 138 : 5S (2018), p. S173. -
További szerzők:Béke Gabriella (1987-) (molekuláris biológus) Angyal Ágnes (1987-) (molekuláris biológus) Kemény Ágnes Pintér Erika Helyes Zsuzsanna Bíró Tamás (1968-) (élettanász) (absztraktok) Mihály Johanna (1982-) (biológus, vegyész)
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
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