CCL

Összesen 2 találat.
#/oldal:
Részletezés:
Rendezés:

1.

001-es BibID:BIBFORM117884
035-os BibID:(cikkazonosító)342162 (WoS)001147540500001 (Scopus)85181709291
Első szerző:Nagy Cynthia (1994-)
Cím:CZE-MS peptide mapping : To desalt or not to desalt? / Cynthia Nagy, Melinda Andrasi, Ruben Szabo, Attila Gaspar
Dátum:2024
ISSN:0003-2670
Megjegyzések:Background: In "shotgun" approaches involving high-performance liquid chromatography or capillary zone electrophoresis (CZE), matrix removal prior to sample analysis is considered as an indispensable tool. Despite the fact that CZE offers a high tolerance towards salts, most publications reported on the use of desalting. There seems to be no clear consensus on the utilization of desalting in the CZE-MS community, most probably due to the absence of works addressing the comparison of desalted and non-desalted digests. Our aim was to fill this research gap using protein samples of varying complexity in different sample matrices. Results: First, standard protein digests were analyzed to build the knowledge on the effect of sample clean-up by solid-phase extraction (SPE) pipette tips and the possible stacking phenomena induced by different sample matrices. Desalting led to a somewhat altered peptide profile, the procedure affected mostly the hydrophilic peptides (although not to a devastating extent). Nevertheless, desalting samples allowed remarkable stacking efficiency owing to their low-conductivity sample background, enabling a so-called field-amplified sample stacking phenomenon. Non-desalted samples also produced a stacking event, the mechanism of which is based on transient-isotachophoresis due to the presence of high-mobility ions in the digestion buffer itself. Adding either extra ammonium ions or acetonitrile into the non-desalted digests enhanced the stacking efficiency. A complex sample (yeast cell lysate) was also analyzed with the optimal conditions, which yielded similar tendencies. Significance: Based on these results, we propose that sample clean-up in the bottom-up sample preparation process prior to CZE-MS analysis can be omitted. The preclusion of desalting can even enhance detection sensitivity, separation efficiency or sequence coverage.
Tárgyszavak:Természettudományok Kémiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Bottom-up proteomics
Capillary zone electrophoresis
Desalting
Mass spectrometry
Peptide mapping
Megjelenés:Analytica Chimica Acta. - 1288 (2024), p. 1-9. -
További szerzők:Andrási Melinda (1979-) (gyógyszerész) Szabó Ruben (1998-) Gáspár Attila (1970-) (vegyész, kémikus)
Pályázati támogatás:K-142134
Egyéb
ÚNKP-22-3-II
Egyéb
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:

2.

001-es BibID:BIBFORM103039
035-os BibID:(WOS)000522084100009 (Scopus)85080151996
Első szerző:Nagy Cynthia (1994-)
Cím:Fabrication of immobilized enzyme reactors with pillar arrays into polydimethylsiloxane microchip / Cynthia Nagy, Adam Kecskemeti, Attila Gaspar
Dátum:2020
ISSN:0003-2670
Megjegyzések:This paper demonstrates the design, efficiency and applicability of a simple and inexpensive microfluidic immobilized enzymatic reactor (IMER) for rapid protein digestion. The high surface-to-volume ratio (S/V) of the reactor was achieved by forming pillars in the channel. It was found that pillar arrays including dimensions of 40 mu m x 40 mu m as pillar diameter and interpillar distance can provide both relatively high S/V and flow rate in the PDMS chip, the fabrication of which was performed by means of soft lithography using average research laboratory infrastructure. CZE peptide maps of IMER-based digestions were compared to peptide maps obtained from standard in-solution digestion of proteins. The peak patterns of the electropherograms and the identified proteins were similar, however, digestion with the IMER requires less than 10 min, while in-solution digestion takes 16 h.
Tárgyszavak:Természettudományok Kémiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Immobilization
Digestion
Peptide mapping
Enzyme reactor
Array of pillars
poly(dimethylsiloxane)
Megjelenés:Analytica Chimica Acta. - 1108 (2020), p. 70-78. -
További szerzők:Kecskeméti Ádám (1992-) (vegyész) Gáspár Attila (1970-) (vegyész, kémikus)
Pályázati támogatás:GINOP-2.3.2-15-2016-00008
GINOP
GINOP-2.3.3-15-2016-00004
GINOP
NKFIH-K127931
Egyéb
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:
Rekordok letöltése1