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001-es BibID:BIBFORM097508
035-os BibID:(cikkazonosító)1078 (WoS)000724148200001 (Scopus)85117925325
Első szerző:Shaw, Abhirup
Cím:BMP7 increases UCP1-dependent and independent thermogenesis with a unique gene expression program in human neck area derived adipocytes / Shaw Abhirup, B. Tóth Beáta, Arianti Rini, Csomós István, Póliska Szilárd, Vámos Attila, Bacsó Zsolt, Győry Ferenc, Fésüs László, Kristóf Endre
Dátum:2021
ISSN:1424-8247
Megjegyzések:White adipocytes contribute to energy storage accumulating lipid droplets, whereas brown and beige adipocytes mainly function in dissipating energy as heat primarily via the action of uncoupling protein 1 (UCP1). Bone morphogenic protein 7 (BMP7) was shown to drive brown adipocyte differentiation in murine interscapular adipose tissue. Here, we performed global RNA-sequencing and functional assays on adipocytes obtained from subcutaneous (SC) and deep-neck (DN) depots of human neck and differentiated with or without BMP7. We found that BMP7 did not influence differentiation but upregulated browning markers, including UCP1 mRNA and protein in SC and DN derived adipocytes. BMP7 also enhanced mitochondrial DNA content, levels of oxidative phosphorylation complex subunits, along with PGC1? and p-CREB upregulation, and fragmentation of mitochondria. Furthermore, both UCP1-dependent proton leak and UCP1-independent, creatine driven substrate cycle coupled thermogenesis were augmented upon BMP7 addition. The gene expression analysis shed light also on possible role of genes unrelated to thermogenesis so far, including ACAN, CRYAB, and ID1, which were amongst the highest upregulated ones by BMP7 treatment in both types of adipocytes. Together, our study shows that BMP7 strongly upregulates thermogenesis in human neck area derived adipocytes, along with genes, which might have a supporting role in energy expenditure.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Pharmaceuticals. - 14 : 11 (2021), p. 1-21. -
További szerzők:Bartáné Tóth Beáta (1970-) (molekuláris biológus) Arianti, Rini (1991-) (biokémikus) Csomós István (1983-) (molekuláris biológus) Póliska Szilárd (1978-) (biológus) Vámos Attila (1991-) (gyógyszer-biotechnológus) Bacsó Zsolt (1963-) (biofizikus) Győry Ferenc (1964-) (sebész) Fésüs László (1947-) (orvos biokémikus) Kristóf Endre (1987-) (általános orvos)
Pályázati támogatás:GINOP-2.3.2-15-2016-00006
GINOP
FK131424
OTKA
K129139
OTKA
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
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2.

001-es BibID:BIBFORM096387
035-os BibID:(cikkazonosító)737872
Első szerző:Shaw, Abhirup
Cím:Irisin stimulates the release of CXCL1 from differentiating human subcutaneous and deep-neck derived adipocytes via upregulation of NF[kappa]B pathway / Abhirup Shaw, Beáta B. Tóth, Róbert Király, Rini Arianti, István Csomós, Szilárd Póliska, Attila Vámos, Ilma R. Korponay-Szabó, Zsolt Bacso, Ferenc Győry, László Fésüs, Endre Kristóf
Dátum:2021
ISSN:2296-634X
Megjegyzések:Thermogenic brown and beige adipocytes might open up new strategies in combating obesity. Recent studies in rodents and humans have indicated that these adipocytes release cytokines, termed "batokines". Irisin was discovered as a polypeptide regulator of beige adipocytes released by myocytes, primarily during exercise. We performed global RNA sequencing on adipocytes derived from human subcutaneous and deep-neck precursors, which were differentiated in the presence or absence of irisin. Irisin did not exert an effect on the expression of characteristic thermogenic genes, while upregulated genes belonging to various cytokine signaling pathways. Out of the several upregulated cytokines, CXCL1, the highest upregulated, was released throughout the entire differentiation period, and predominantly by differentiated adipocytes. Deep-neck area tissue biopsies also showed a significant release of CXCL1 during 24 hours irisin treatment. Gene expression data indicated upregulation of the NF?B pathway upon irisin treatment, which was validated by an increase of p50 and decrease of I?B? protein level, respectively. Continuous blocking of the NF?B pathway, using a cell permeable inhibitor of NF?B nuclear translocation, significantly reduced CXCL1 release. The released CXCL1 exerted a positive effect on the adhesion of endothelial cells. Together, our findings demonstrate that irisin stimulates the release of a novel adipokine, CXCL1, via upregulation of NF?B pathway in neck area derived adipocytes, which might play an important role in improving tissue vascularization.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Frontiers in Cell and Developmental Biology. - 9 (2021), p. 1-19. -
További szerzők:Bartáné Tóth Beáta (1970-) (molekuláris biológus) Király Róbert (1975-) (biológus) Arianti, Rini (1991-) (biokémikus) Csomós István (1983-) (molekuláris biológus) Póliska Szilárd (1978-) (biológus) Vámos Attila (1991-) (gyógyszer-biotechnológus) Korponay-Szabó Ilma (1959-) (gyermekgyógyász) Bacsó Zsolt (1963-) (biofizikus) Győry Ferenc (1964-) (sebész) Fésüs László (1947-) (orvos biokémikus) Kristóf Endre (1987-) (általános orvos)
Pályázati támogatás:GINOP-2.3.2-15-2016-00006
GINOP
FK131424
OTKA
K129139
OTKA
K120392
OTKA
ÚNKP-20-5-DE-12
Egyéb
BO/00042/18/8
MTA
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Intézményi repozitóriumban (DEA) tárolt változat
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3.

001-es BibID:BIBFORM116662
035-os BibID:(Scopus)85171172955 (WoS)001023372700001 (cikkazonosító)1249909
Első szerző:Vámos Attila (gyógyszer-biotechnológus)
Cím:Corrigendum : Human abdominal subcutaneous-derived active beige adipocytes carrying FTO rs1421085 obesity-risk alleles exert lower thermogenic capacity / Vámos Attila, Arianti Rini, Vinnai Boglárka Ágnes, Alrifai Rahaf, Shaw Abhirup, Póliska Szilárd, Guba Andrea, Csősz Éva, Csomós István, Mocsár Gábor, Lányi Cecilia, Balajthy Zoltán, Fésüs László, Kristóf Endre
Dátum:2023
ISSN:2296-634X
Megjegyzések:In the published article, there was an error. In the published article, the Reference "Bjune et al., 2005" was cited with an incorrect year of publication. The correct year of publication is 2019. In the published article "Bjune, J. I., Haugen, C., Gudbrandsen, O., Nordb?, O. P., Nielsen, H. J., V?age, V., et al. (2019). IRX5 regulates adipocyte amyloid precursor protein and mitochondrial respiration in obesity. Int J Obes (Lond)., 43(11), 2151?2162. https://doi.org/10.1038/s41366-018-0275-y" was not referenced in the article. The reference has now been inserted into the article. A correction has been made to the Introduction. This sentence previously stated: "In addition, IRX5 silencing increased the mitochondrial respiration in isolated mouse adipocytes (Bjune et al., 2005)." The corrected sentence appears below: "In addition, IRX5 silencing increased the mitochondrial respiration in isolated mouse adipocytes (Bjune et al., 2019)." The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated. Copyright ? 2023 Vámos, Arianti, Vinnai, Alrifai, Shaw, Póliska, Guba, Csősz, Csomós, Mocsár, Lányi, Balajthy, Fésüs and Kristóf.
Tárgyszavak:Orvostudományok Elméleti orvostudományok hozzászólás
folyóiratcikk
adipocytes
beige
FTO rs1421085
obesity
SLC7A10
thermogenesis
UCP 1
Megjelenés:Frontiers in Cell and Developmental Biology. - 11 (2023), p. 1-2. -
További szerzők:Arianti, Rini (1991-) (biokémikus) Vinnai Boglárka Ágnes (1996-) (molekuláris biológus) Alrifai, Rahaf Shaw, Abhirup (1992-) Póliska Szilárd (1978-) (biológus) Guba Andrea (1975-) (Okleveles vegyész) Csősz Éva (1977-) (biokémikus, molekuláris biológus) Csomós István (1983-) (molekuláris biológus) Mocsár Gábor (1981-) (biofizikus) Lányi Cecília Balajthy Zoltán (1957-) (biokémikus, sejtbiológus) Fésüs László (1947-) (orvos biokémikus) Kristóf Endre (1987-) (általános orvos)
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Intézményi repozitóriumban (DEA) tárolt változat
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4.

001-es BibID:BIBFORM112108
035-os BibID:(cikkazonosító)1155673 (scopus)85164495947 (wos)001023372700001
Első szerző:Vámos Attila (gyógyszer-biotechnológus)
Cím:Human Abdominal Subcutaneous-Derived Active Beige Adipocytes Carrying FTO rs1421085 Obesity-Risk Alleles Exert Lower Thermogenic Capacity / Vámos Attila, Arianti Rini, Vinnai Boglárka Ágnes, Alrifai Rahaf, Shaw Abhirup, Póliska Szilárd, Guba Andrea, Csősz Éva, Csomós István, Mocsár Gábor, Lányi Cecília, Balajthy Zoltán, Fésüs László, Kristóf Endre
Dátum:2023
ISSN:2296-634X
Megjegyzések:White adipocytes store lipids, have a large lipid droplet and few mitochondria. Brown and beige adipocytes, which produce heat, are characterized by high expression of uncoupling protein (UCP) 1, multilocular lipid droplets, and large amounts of mitochondria. The rs1421085 T-to-C single-nucleotide polymorphism (SNP) of the human FTO gene interrupts a conserved motif for ARID5B repressor, resulting in adipocyte type shift from beige to white. We obtained abdominal subcutaneous adipose tissue from donors carrying FTO rs1421085 TT (risk-free) or CC (obesity-risk) genotypes, isolated and differentiated their preadipocytes into beige adipocytes (driven by the PPAR? agonist rosiglitazone for 14 days), and activated them with dibutyryl-cAMP for 4 hours. Then, either the same culture conditions were applied for additional 14 days (active beige adipocytes) or it was replaced by a white differentiation medium (inactive beige adipocytes). White adipocytes were differentiated by their medium for 28 days. RNA-sequencing was performed to investigate the gene expression pattern of adipocytes carrying different FTO alleles and found that active beige adipocytes had higher brown adipocyte content and browning capacity compared to white or inactive beige ones when the cells were obtained from risk-free TT but not from obesity-risk CC genotype carriers. Active beige adipocytes carrying FTO CC had lower thermogenic gene (e.g., UCP1, PM20D1, CIDEA) expression and thermogenesis measured by proton leak respiration as compared to TT carriers. In addition, active beige adipocytes with CC alleles exerted lower expression of ASC-1 neutral amino acid transporter (encoded by SLC7A10) and less consumption of Ala, Ser, Cys, and Gly as compared to risk-free carriers. We did not observe any influence of the FTO rs1421085 SNP on white and inactive beige adipocytes highlighting its exclusive and critical effect when adipocytes were activated for thermogenesis.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
adipocytes
beige
obesity
FTO rs1421085
thermogenesis
UCP1
SLC7A10
Megjelenés:Frontiers in Cell and Developmental Biology. - 11 (2023), p. 1-18. -
További szerzők:Arianti, Rini (1991-) (biokémikus) Vinnai Boglárka Ágnes (1996-) (molekuláris biológus) Alrifai, Rahaf Shaw, Abhirup (1992-) Póliska Szilárd (1978-) (biológus) Guba Andrea (1975-) (Okleveles vegyész) Csősz Éva (1977-) (biokémikus, molekuláris biológus) Csomós István (1983-) (molekuláris biológus) Mocsár Gábor (1981-) (biofizikus) Lányi Cecília Balajthy Zoltán (1957-) (biokémikus, sejtbiológus) Fésüs László (1947-) (orvos biokémikus) Kristóf Endre (1987-) (általános orvos)
Pályázati támogatás:FK131424
OTKA
K129139
OTKA
ÚNKP-22-3-I-DE-30
Egyéb
ÚNKP-22-3-II-DE-25
Egyéb
Internet cím:Szerző által megadott URL
Intézményi repozitóriumban (DEA) tárolt változat
DOI
Borító:

5.

001-es BibID:BIBFORM101024
035-os BibID:(cikkazonosító)363 (WoS)000774617800001 (Scopus)85127543748
Első szerző:Vámos Attila (gyógyszer-biotechnológus)
Cím:Mitophagy Mediates the Beige to White Transition of Human Primary Subcutaneous Adipocytes Ex Vivo / Vámos Attila, Shaw Abhirup, Varga Klára, Csomós István, Mocsár Gábor, Balajthy Zoltán, Lányi Cecília, Bacso Zsolt, Szatmári-Tóth Mária, Kristóf Endre
Dátum:2022
ISSN:1424-8247
Megjegyzések:Brown and beige adipocytes have multilocular lipid droplets, express uncoupling protein (UCP) 1, and promote energy expenditure. In rodents, when the stimulus of browning subsides, parkin-dependent mitophagy is activated and dormant beige adipocytes persist. In humans, however, the molecular events during the beige to white transition have not been studied in detail. In this study, human primary subcutaneous abdominal preadipocytes were differentiated to beige for 14 days, then either the beige culture conditions were applied for an additional 14 days or it was replaced by a white medium. Control white adipocytes were differentiated by their specific cocktail for 28 days. Peroxisome proliferator-activated receptor ?-driven beige differentiation resulted in increased mitochondrial biogenesis, UCP1 expression, fragmentation, and respiration as compared to white. Morphology, UCP1 content, mitochondrial fragmentation, and basal respiration of the adipocytes that underwent transition, along with the induction of mitophagy, were similar to control white adipocytes. However, white converted beige adipocytes had a stronger responsiveness to dibutyril-cAMP, which mimics adrenergic stimulus, than the control white ones. Gene expression patterns showed that the removal of mitochondria in transitioning adipocytes may involve both parkin-dependent and -independent pathways. Preventing the entry of beige adipocytes into white transition can be a feasible way to maintain elevated thermogenesis and energy expenditure.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
obesity
beige adipocytes
mitophagy
thermogenesis
uncoupling protein 1
parkin
Megjelenés:Pharmaceuticals. - 15 : 3 (2022), p. 1-21. -
További szerzők:Shaw, Abhirup (1992-) Varga Klára Csomós István (1983-) (molekuláris biológus) Mocsár Gábor (1981-) (biofizikus) Balajthy Zoltán (1957-) (biokémikus, sejtbiológus) Lányi Cecília Bacsó Zsolt (1963-) (biofizikus) Szatmári-Tóth Mária (1987-) (molekuláris biológus) Kristóf Endre (1987-) (általános orvos)
Pályázati támogatás:FK131424
OTKA
EFOP-3.6.3-VEKOP-16-2017-00009
EFOP
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
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