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001-es BibID:BIBFORM078411
035-os BibID:(PMID)30734834 (WoS)000472512500009 (Scopus)85061242629
Első szerző:Baksa Brigitta (fogorvos)
Cím:Characterization of functional subgroups among genetically identified cholinergic neurons in the pedunculopontine nucleus / Baksa B., Kovács A., Bayasgalan T., Szentesi P., Kőszeghy Á., Szücs P., Pál B.
Dátum:2019
ISSN:1420-682X
Megjegyzések:The pedunculopontine nucleus (PPN) is a part of the reticular activating system which is composed of cholinergic, glutamatergic and GABAergic neurons. Early electrophysiological studies characterized and grouped PPN neurons based on certain functional properties (i.e. the presence or absence of the A-current, spike latency, and low threshold spikes). Although other electrophysiological characteristics of these neurons were also described (as high threshold membrane potential oscillations, great differences in spontaneous firing rate and the presence or absence of the M-current), systematic assessment of these properties and correlation of them with morphological markers are still missing. In this work, we conducted electrophysiological experiments on brain slices of genetically identified cholinergic neurons in the PPN. Electrophysiological properties were compared with rostrocaudal location of the neuronal soma and selected morphometric features obtained with post hoc reconstruction. We found that functional subgroups had different proportions in the rostral and caudal subregions of the nucleus. Neurons with A-current can be divided to early-firing and late-firing neurons, where the latter type was found exclusively in the caudal subregion. Similar to this, different parameters of high threshold membrane potential oscillations also showed characteristic rostrocaudal distribution. Furthermore, based on our data we propose that high threshold oscillations rather emerge from neuronal somata and not from the proximal dendrites. In summary, we demonstrated the existence and spatial distribution of functional subgroups of genetically identified PPN cholinergic neurons, which are in accordance with differences found in projection and in vivo functional findings of the subregions. Being aware of functional differences of PPN subregions will help the design and analysis of experiments using genetically encoded opto- and chemogenetic markers for in vivo experiments.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
pedunculopontine nucleus
cholinergic neuron
spike delay
A-current
rostrocaudal gradient
high threshold oscillation
Megjelenés:Cellular And Molecular Life Sciences. - 76 : 14 (2019), p. 2799-2815. -
További szerzők:Kovács Adrienn (1989-) (molekuláris biológus) Bayasgalan, Tsogbadrakh (1983-) (Általános orvos) Szentesi Péter (1967-) (élettanász) Kőszeghy Áron (1983-) (Ph.D hallgató, élettanász) Szűcs Péter (1974-) (kutatóorvos) Pál Balázs (1975-) (élettanász)
Pályázati támogatás:KTIA_13_NAP-A-I/10
Egyéb
2017-1.2.1-NKP-2017-00002
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DOI
Intézményi repozitóriumban (DEA) tárolt változat
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2.

001-es BibID:BIBFORM082078
035-os BibID:(WoS)000509325000003 (Scopus)85071744080 (PubMed)31469725
Első szerző:Kovács Adrienn (molekuláris biológus)
Cím:Orexinergic actions modify occurrence of slow inward currents on neurons in the pedunculopontine nucleus / Kovács Adrienn, Baksa Brigitta, Bayasgalan Tsogbadrakh, Szentesi Péter, Csemer Andrea, Pál Balázs
Dátum:2019
ISSN:0959-4965
Megjegyzések:Orexins are neuromodulatory peptides of the lateral hypothalamus which regulate homeostatic mechanisms including sleep-wakefulness cycles. Orexinergic actions stabilize wakefulness by acting on the nuclei of the reticular activating system, including the pedunculopontine nucleus. Orexin application to pedunculopontine neurons produces a noisy tonic inward current and an increase in the frequency and amplitudes of excitatory postsynaptic currents. In the present project, we investigated orexinergic neuromodulatory actions on astrocyte-mediated neuronal slow inward currents of pedunculopontine neurons and their relationships with tonic currents by using slice electrophysiology on preparations from mice. We demonstrated that, in contrast to several other neuromodulatory actions and in line with literature data, orexin predominantly elicited a tonic inward current. A subpopulation of the pedunculopontine neurons possessed slow inward currents. Independently from the tonic currents, actions on slow inward currents were also detected, which resembled other neuromodulatory actions: if slow inward currents were almost absent on the neuron, orexin induced an increase of the charge movements by slow inward currents, whereas if slow inward current activity was abundant on the neurons, orexin exerted inhibitory action on it. Our data support the previous findings that orexin elicits only inward currents in contrast with cannabinoid, cholinergic or serotonergic actions. Similar to the aforementioned neuromodulatory actions, orexin influences slow inward currents in a way depending on control slow inward current activity. Furthermore, we found that orexinergic actions on slow inward currents are similarly independent from its actions on tonic currents, as it was previously found with other neuromodulatory agonists.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
neuromodulation
orexin
pedunculopontine nucleus
slow inward current
tonic inward current
Megjelenés:Neuroreport. - 30 : 14 (2019), p. 933-938. -
További szerzők:Baksa Brigitta (1989-) (fogorvos) Bayasgalan, Tsogbadrakh (1983-) (Általános orvos) Szentesi Péter (1967-) (élettanász) Csemer Andrea (1994-) (molekuláris biológus) Pál Balázs (1975-) (élettanász)
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:
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