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001-es BibID:BIBFORM073109
Első szerző:Nagy Zsolt
Cím:MICAN, a new fluorophore for vital and non-vital staining of human cells / Nagy Zsolt, Nagy Miklós, Kiss Alexandra, Rácz Dávid, Barna Beatrix, Könczöl Péter, Bankó Csaba, Bacsó Zsolt, Kéki Sándor, Banfalvi Gaspar, Szemán-Nagy Gábor
Dátum:2018
ISSN:0887-2333
Megjegyzések:Fluorescence time-lapse microscopy is in connection with the invasive properties of fluorochrome applied, andwith the toxicity of the excitation energy and wavelength of the dye itself. Experiments with the newly synthesizedfluorescent dye 1-N-methylamino-5-isocyanonaphthalene (MICAN) served to test its cytotoxicity onhuman HaCaT keratinocyte cell cultures. Experiments related to staining capability were performed with paraformaldehyde(PFA) fixed cells and observed with fluorescence microscope. It was assumed that the fluorophore1-amino-5-isocyanonaphthalene (ICAN) and especially its N-methylamino derivative MICAN, containingcondensed aromatic rings could serve as a nonselective fluorescent dye capable to stain cellular structures offixed, living, damaged and dead cells. This notion was confirmed by the MICAN staining of cytoplasmic proteinsprimarily rough endoplasmic reticulum (RER), smooth endoplasmic reticulum (SEM) and less efficiently nuclearproteins suggesting the involvement of staining of subcellular structures involved in protein synthesis. MICANwas not only well tolerated by living cells but turned out to be a strong heterochromatin and RER staining agent.This led to the development of a MICAN staining protocol for native and living samples. Relative to otherfluorescent dyes, MICAN is not only useful but also cost-effective. Toxicology tests were performed using 30, 10,5, 0.5 ?g/ml MICAN concentrations. Time-lapse videomicroscopy at near-infrared (NIR) illumination has beenused for the examination of MICAN effect on cell division. It was found that MICAN as a vital stain had nosignificant harmful effect on HaCaT cells. MICAN turned out to be a non-toxic, highly quantum-efficient vitalstain with minimal, or no photobleaching, and can be applied to co-stain with propidium-iodide due the strongspectral separation.
Tárgyszavak:Természettudományok Kémiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Fluorescence imaging
Solvatochromism
Vital staining
Isocyanide
MICAN
Megjelenés:Toxicology In Vitro. - 48 (2018), p. 137-145. -
További szerzők:Nagy Miklós (1976-) (vegyész) Kiss Alexandra (1994-) (gyógyszer-biotechnológus) Rácz Dávid (1987-) (vegyész) Barna Beatrix Könczöl Péter (1972-) (nemesítő) Bankó Csaba (1989-) (molekuláris biológus) Bacsó Zsolt (1963-) (biofizikus) Kéki Sándor (1964-) (polimer kémikus) Bánfalvi Gáspár (1943-) (sejtbiológus, gyógyszerész) Szemán-Nagy Gábor (1975-) (biológia tanár-molekuláris biológus)
Pályázati támogatás:GINOP-2.3.2-15-2016-00041
GINOP
116465
OTKA
János Bolyai Research Scholarship of the Hungarian Academy of Sciences
MTA
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