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001-es BibID:BIBFORM111915
035-os BibID:(cikkazonosító)109385 (scopus)85161297715
Első szerző:Arianti, Rini (biokémikus)
Cím:Availability of abundant thiamine determines efficiency of thermogenic activation in human neck area derived adipocytes / Arianti Rini, Vinnai Boglárka Ágnes, Győry Ferenc, Guba Andrea, Csősz Éva, Kristóf Endre, Fésüs László
Dátum:2023
ISSN:0955-2863
Megjegyzések:Brown/beige adipocytes express uncoupling protein-1 (UCP1) that enables them to dissipate energy as heat. Systematic activation of this process can alleviate obesity. Human brown adipose tissues are interspersed in distinct anatomical regions including deep neck. We found that UCP1 enriched adipocytes differentiated from precursors of this depot highly expressed ThTr2 transporter of thiamine and consumed thiamine during thermogenic activation of these adipocytes by cAMP which mimics adrenergic stimulation. Inhibition of ThTr2 led to lower thiamine consumption with decreased proton leak respiration reflecting reduced uncoupling. In the absence of thiamine, cAMP-induced uncoupling was diminished but restored by thiamine addition reaching the highest levels at thiamine concentrations larger than present in human blood plasma. Thiamine is converted to thiamine pyrophosphate (TPP) in cells; the addition of TPP to permeabilized adipocytes increased uncoupling fueled by TPP-dependent pyruvate dehydrogenase. ThTr2 inhibition also hampered cAMP-dependent induction of UCP1, PGC1a, and other browning marker genes, and thermogenic induction of these genes was potentiated by thiamine in a concentration dependent manner. Our study reveals the importance of amply supplied thiamine during thermogenic activation in human adipocytes which provides TPP for TPP-dependent enzymes not fully saturated with this cofactor and by potentiating the induction of thermogenic genes.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
human adipocytes
thiamine
thiamine transporter
pyruvate dehydrogenase
thermogenesis
UCP1 expression
Megjelenés:Journal Of Nutritional Biochemistry. - 119 (2023), p. 1-13. -
További szerzők:Vinnai Boglárka Ágnes (1996-) (molekuláris biológus) Győry Ferenc (1964-) (sebész) Guba Andrea (1975-) (Okleveles vegyész) Csősz Éva (1977-) (biokémikus, molekuláris biológus) Kristóf Endre (1987-) (általános orvos) Fésüs László (1947-) (orvos biokémikus)
Pályázati támogatás:GINOP-2.3.2-15-2016-00006
GINOP
FK131424
OTKA
K129139
OTKA
ÚNKP-22-3-I
Egyéb
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Intézményi repozitóriumban (DEA) tárolt változat
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2.

001-es BibID:BIBFORM096051
035-os BibID:(WoS)000676122000001 (Scopus)85111301797
Első szerző:Arianti, Rini (biokémikus)
Cím:ASC-1 transporter-dependent amino acid uptake is required for the efficient thermogenic response of human adipocytes to adrenergic stimulation / Rini Arianti, Boglarka Agnes Vinnai, Beata B. Toth, Abhirup Shaw, Eva Csosz, Attila Vamos, Ferenc Gyory, Pamela Fischer-Posovszky, Martin Wabitsch, Endre Kristof, Laszlo Fesus
Dátum:2021
ISSN:0014-5793
Megjegyzések:Brown and beige adipocytes dissipate energy by uncoupling protein 1 (UCP1)-dependent and UCP1-independent thermogenesis, which may be utilized to develop treatments against obesity. We have found that mRNA and protein expression of the alanine/serine/cysteine transporter-1 (ASC-1) was induced during adipocyte differentiation of human brown-prone deep neck and beige-competent subcutaneous neck progenitors, and SGBS preadipocytes. cAMP stimulation of differentiated adipocytes led to elevated uptake of serine, cysteine, and glycine, in parallel with increased oxygen consumption, augmented UCP1-dependent proton leak, increased creatine-driven substrate cycle-coupled respiration, and upregulation of thermogenesis marker genes and several respiratory complex subunits; these outcomes were impeded in the presence of the specific ASC-1 inhibitor, BMS-466442. Our data suggest that ASC-1-dependent consumption of serine, cysteine, and glycine is required for efficient thermogenic stimulation of human adipocytes.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
adipocytes
ASC-1 inhibition
gene expression
obesity
proton leak respiration
thermogenesis
uncoupling protein
Megjelenés:Febs Letters. - 595 : 16 (2021), p. 2085-2098. -
További szerzők:Vinnai Boglárka Ágnes (1996-) (molekuláris biológus) Bartáné Tóth Beáta (1970-) (molekuláris biológus) Shaw, Abhirup (1992-) Csősz Éva (1977-) (biokémikus, molekuláris biológus) Vámos Attila (1991-) (gyógyszer-biotechnológus) Győry Ferenc (1964-) (sebész) Fischer-Posovszky Pamela Wabitsch, Martin Kristóf Endre (1987-) (általános orvos) Fésüs László (1947-) (orvos biokémikus)
Pályázati támogatás:GINOP-2.3.2-15-2016-00006
GINOP
EFOP-3.6.3-VEKOP-16-2017-00009
EFOP
FK131424
OTKA
K129139
OTKA
ÚNKP-20-5-DE-12
Egyéb
ÚNKP-20-2-I-DE-187
Egyéb
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Intézményi repozitóriumban (DEA) tárolt változat
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3.

001-es BibID:BIBFORM097508
035-os BibID:(cikkazonosító)1078 (WoS)000724148200001 (Scopus)85117925325
Első szerző:Shaw, Abhirup
Cím:BMP7 increases UCP1-dependent and independent thermogenesis with a unique gene expression program in human neck area derived adipocytes / Shaw Abhirup, B. Tóth Beáta, Arianti Rini, Csomós István, Póliska Szilárd, Vámos Attila, Bacsó Zsolt, Győry Ferenc, Fésüs László, Kristóf Endre
Dátum:2021
ISSN:1424-8247
Megjegyzések:White adipocytes contribute to energy storage accumulating lipid droplets, whereas brown and beige adipocytes mainly function in dissipating energy as heat primarily via the action of uncoupling protein 1 (UCP1). Bone morphogenic protein 7 (BMP7) was shown to drive brown adipocyte differentiation in murine interscapular adipose tissue. Here, we performed global RNA-sequencing and functional assays on adipocytes obtained from subcutaneous (SC) and deep-neck (DN) depots of human neck and differentiated with or without BMP7. We found that BMP7 did not influence differentiation but upregulated browning markers, including UCP1 mRNA and protein in SC and DN derived adipocytes. BMP7 also enhanced mitochondrial DNA content, levels of oxidative phosphorylation complex subunits, along with PGC1? and p-CREB upregulation, and fragmentation of mitochondria. Furthermore, both UCP1-dependent proton leak and UCP1-independent, creatine driven substrate cycle coupled thermogenesis were augmented upon BMP7 addition. The gene expression analysis shed light also on possible role of genes unrelated to thermogenesis so far, including ACAN, CRYAB, and ID1, which were amongst the highest upregulated ones by BMP7 treatment in both types of adipocytes. Together, our study shows that BMP7 strongly upregulates thermogenesis in human neck area derived adipocytes, along with genes, which might have a supporting role in energy expenditure.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Pharmaceuticals. - 14 : 11 (2021), p. 1-21. -
További szerzők:Bartáné Tóth Beáta (1970-) (molekuláris biológus) Arianti, Rini (1991-) (biokémikus) Csomós István (1983-) (molekuláris biológus) Póliska Szilárd (1978-) (biológus) Vámos Attila (1991-) (gyógyszer-biotechnológus) Bacsó Zsolt (1963-) (biofizikus) Győry Ferenc (1964-) (sebész) Fésüs László (1947-) (orvos biokémikus) Kristóf Endre (1987-) (általános orvos)
Pályázati támogatás:GINOP-2.3.2-15-2016-00006
GINOP
FK131424
OTKA
K129139
OTKA
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
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4.

001-es BibID:BIBFORM096387
035-os BibID:(cikkazonosító)737872
Első szerző:Shaw, Abhirup
Cím:Irisin stimulates the release of CXCL1 from differentiating human subcutaneous and deep-neck derived adipocytes via upregulation of NF[kappa]B pathway / Abhirup Shaw, Beáta B. Tóth, Róbert Király, Rini Arianti, István Csomós, Szilárd Póliska, Attila Vámos, Ilma R. Korponay-Szabó, Zsolt Bacso, Ferenc Győry, László Fésüs, Endre Kristóf
Dátum:2021
ISSN:2296-634X
Megjegyzések:Thermogenic brown and beige adipocytes might open up new strategies in combating obesity. Recent studies in rodents and humans have indicated that these adipocytes release cytokines, termed "batokines". Irisin was discovered as a polypeptide regulator of beige adipocytes released by myocytes, primarily during exercise. We performed global RNA sequencing on adipocytes derived from human subcutaneous and deep-neck precursors, which were differentiated in the presence or absence of irisin. Irisin did not exert an effect on the expression of characteristic thermogenic genes, while upregulated genes belonging to various cytokine signaling pathways. Out of the several upregulated cytokines, CXCL1, the highest upregulated, was released throughout the entire differentiation period, and predominantly by differentiated adipocytes. Deep-neck area tissue biopsies also showed a significant release of CXCL1 during 24 hours irisin treatment. Gene expression data indicated upregulation of the NF?B pathway upon irisin treatment, which was validated by an increase of p50 and decrease of I?B? protein level, respectively. Continuous blocking of the NF?B pathway, using a cell permeable inhibitor of NF?B nuclear translocation, significantly reduced CXCL1 release. The released CXCL1 exerted a positive effect on the adhesion of endothelial cells. Together, our findings demonstrate that irisin stimulates the release of a novel adipokine, CXCL1, via upregulation of NF?B pathway in neck area derived adipocytes, which might play an important role in improving tissue vascularization.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Frontiers in Cell and Developmental Biology. - 9 (2021), p. 1-19. -
További szerzők:Bartáné Tóth Beáta (1970-) (molekuláris biológus) Király Róbert (1975-) (biológus) Arianti, Rini (1991-) (biokémikus) Csomós István (1983-) (molekuláris biológus) Póliska Szilárd (1978-) (biológus) Vámos Attila (1991-) (gyógyszer-biotechnológus) Korponay-Szabó Ilma (1959-) (gyermekgyógyász) Bacsó Zsolt (1963-) (biofizikus) Győry Ferenc (1964-) (sebész) Fésüs László (1947-) (orvos biokémikus) Kristóf Endre (1987-) (általános orvos)
Pályázati támogatás:GINOP-2.3.2-15-2016-00006
GINOP
FK131424
OTKA
K129139
OTKA
K120392
OTKA
ÚNKP-20-5-DE-12
Egyéb
BO/00042/18/8
MTA
Internet cím:Szerző által megadott URL
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Intézményi repozitóriumban (DEA) tárolt változat
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5.

001-es BibID:BIBFORM114025
035-os BibID:(cikkazonosító)1207394
Első szerző:Vinnai Boglárka Ágnes (molekuláris biológus)
Cím:Extracellular thiamine concentration influences thermogenic competency of differentiating neck area-derived human adipocytes / Vinnai Boglárka Ágnes, Arianti Rini, Győry Ferenc, Bacsó Zsolt, Fésüs László, Kristóf Endre
Dátum:2023
ISSN:2296-861X
Megjegyzések:Brown adipose tissue (BAT) dissipates energy in the form of heat majorly via the mitochondrial uncoupling protein 1 (UCP1). The activation of BAT, which is enriched in the neck area and contains brown and beige adipocytes in humans, was considered as a potential therapeutic target to treat obesity. Therefore, finding novel agents that can stimulate the differentiation and recruitment of brown or beige thermogenic adipocytes are important subjects for investigation. The current study investigated how the availability of extracellular thiamine (vitamin B1), an essential cofactor of mitochondrial enzyme complexes that catalyze key steps in the catabolism of nutrients, affects the expression of thermogenic marker genes and proteins and subsequent functional parameters during ex vivo adipocyte differentiation. Therefore, we differentiated primary human adipogenic progenitors that were cultivated from subcutaneous (SC) or deep neck (DN) adipose tissues in the presence of gradually increasing thiamine concentrations during their 14? day long differentiation program. Higher thiamine levels resulted in increased expression of thiamine transporter 1 and 2 both at mRNA and protein levels in human neck area-derived adipocytes. Gradually increasing concentrations of thiamine led to increased basal, cAMP-stimulated, and proton-leak respiration along with elevated mitochondrial biogenesis of the differentiated adipocytes. The extracellular thiamine availability during adipogenesis determined the expression levels of UCP1, PGC1a, CKMT2, and other browning-related genes and proteins in primary SC and DN-derived adipocytes in a concentration-dependent manner. Providing abundant amounts of thiamine further increased the thermogenic competency of the adipocytes. Our study raises the possibility of a novel strategy with long-term thiamine supplementation, which can enhance the thermogenic competency of differentiating neck area-derived adipocytes for preventing or combating obesity.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
obesity
adipocytes
beige
thermogenesis
thiamine
UCP1
Megjelenés:Frontiers in Nutrition. - 10 (2023), p. 1-15. -
További szerzők:Arianti, Rini (1991-) (biokémikus) Győry Ferenc (1964-) (sebész) Bacsó Zsolt (1963-) (biofizikus) Fésüs László (1947-) (orvos biokémikus) Kristóf Endre (1987-) (általános orvos)
Pályázati támogatás:FK131424
OTKA
K129139
OTKA
ÚNKP-22-3-I
Egyéb
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
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