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001-es BibID:BIBFORM104367
035-os BibID:(WOS)000624483300001 (Scopus)85101959071
Első szerző:Ellouze, Mariem
Cím:Modeling Bacillus cereus Growth and Cereulide Formation in Cereal-, Dairy-, Meat-, Vegetable-Based Food and Culture Medium / Mariem Ellouze, Nathália Buss Da Silva, Katia Rouzeau-Szynalski, Laura Coisne, Frédérique Cantergiani, József Baranyi
Dátum:2021
ISSN:1664-302X
Megjegyzések:This study describes the simultaneous Bacillus cereus growth and cereulide formation, in culture medium and cereal-, dairy-, meat-, and vegetable-based food matrices. First, bacterial growth experiments were carried out under a wide range of temperatures (from 9 to 45 C), using the emetic reference strain F4810/72, in the above-mentioned matrices. Then, the generated data were put in a modeling framework where the response variable was a vector of two components: the concentration of B. cereus and that of its toxin, cereulide. Both were considered time-, temperature- and matrixdependent. The modeling was carried out in a series of steps: the parameters fitted in one step became the response variable of the following step. Using the square root link function, the maximum specific growth rate of the organism and the time to the appearance of quantifiable cereulide were modeled against temperature by cardinal parameters models (CPM), for each matrix. Finally, a validation study was carried out on an independent data set obtained in the same matrices and using various Bacillus cereus strains. Results showed that both growth and toxin-formation depended on the food matrix and on the environment but not in the same way. Thus, the matrix (culture medium), where the highest growth rate of B. cereus was observed, was not the medium where the shortest time to quantifiable cereulide occurred. While the cerealbased matrix generated the smallest growth rates (0.41-times smaller than culture medium did), quantifiable cereulide appeared in it at earlier times compared to the other tested matrices. In fact, three groups of matrices could be distinguished based on their ability to support cereulide formation (1) the cereal-based matrix (highest), (2) the culture medium and the dairy-based matrix (intermediate), and (3) the meat- and vegetablebased matrices (lowest). This ranking between the matrices is quite different from that based on their suitability to the growth of the organism. Our models can be used in HACCP studies, to improve shelf-life predictions and, generally, microbiological food safety assessments of products for which B. cereus is the main concern.
Tárgyszavak:Agrártudományok Élelmiszertudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Frontiers in Microbiology. - 12 (2021), p. 1-13. -
További szerzők:Buss da Silva, Nathália Rouzeau-Szynalski, Katia Coisne, Laura Cantergiani, Frédérique Baranyi József (1956-) (biomatematikus)
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2.

001-es BibID:BIBFORM104359
035-os BibID:(WOS)000704698300001 (Scopus)85116585425 (cikkazonosító)755736
Első szerző:Ellouze, Mariem
Cím:Corrigendum: Modeling Bacillus cereus Growth and Cereulide Formation in Cereal-, Dairy-, Meat-, Vegetable-Based Food and Culture Medium / Mariem Ellouze, Nathália Buss Da Silva, Katia Rouzeau-Szynalski, Laura Coisne, Frédérique Cantergiani, József Baranyi
Dátum:2021
ISSN:1664-302X
Tárgyszavak:Agrártudományok Élelmiszertudományok idegen nyelvű folyóiratközlemény hazai lapban
folyóiratcikk
Bacillus cereus
growth
cereulide formation
predictive microbiology
food
culture medium
Megjelenés:Frontiers in Microbiology. - 12 (2021), p. 1. -
További szerzők:Buss da Silva, Nathália Rouzeau-Szynalski, Katia Coisne, Laura Cantergiani, Frédérique Baranyi József (1956-) (biomatematikus)
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DOI
Intézményi repozitóriumban (DEA) tárolt változat
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3.

001-es BibID:BIBFORM107477
035-os BibID:(cikkazonosító)901484 (WOS)000886959400001 (Scopus)85134918411
Első szerző:Rukit, Junyaluck
Cím:Roles of alternative sigma factors in invasion and growth characteristics of Listeria monocytogenes 10403S into human epithelial colorectal adenocarcinoma Caco-2 cell / Junyaluck Rukit, Atsadang Boonmee, Teeratas Kijpornyongpan, Kan Tulsook, József Baranyi, Soraya Chaturongakul
Dátum:2022
ISSN:1664-302X
Megjegyzések:Listeria monocytogenes is a Gram-positive facultative intracellular bacterium with a broad host range. With its housekeeping sigma factor and four alternative ones (namely SigB, SigC, SigH, and SigL), L. monocytogenes can express genes in response to changing environments. However, the roles of these sigma factors in intracellular survival are still unclear. The objectives of this study were to characterize the role of each alternative sigma factor on L. monocytogenes invasion and growth inside human epithelial colorectal adenocarcinoma Caco-2 cells. We used L. monocytogenes 10403S wild type and its 15 alternative sigma factor deletion mutants at a multiplicity of infection of 100 and 1 in invasion and intracellular growth assays in the Caco-2 cells, respectively. At 1.5, 2, 4, 6, 8, 10, and 12 h post-infection, Caco-2 cells were lysed, and intracellular L. monocytogenes were enumerated on brain-heart infusion agar. Colony-forming and growth rates were compared among strains. The results from phenotypic characterization confirmed that (i) SigB is the key factor for L. monocytogenes invasion and (ii) having only SigA (Delta sigBCHL strain) is sufficient to invade and multiply in the host cell at similar levels as the wild type. Our previous study suggested the negative role of SigL in bile stress response. In this study, we have shown that additional deletion of the rpoN (or sigL) gene to Delta sigB, Delta sigC, or Delta sigH could restore the impaired invasion efficiencies of the single mutant, suggesting the absence of SigL could enhance host invasion. Therefore, we further investigated the role of SigL during extracellular and intracellular life cycles. Using RNA sequencing, we identified 118 and 16 SigL-dependent genes during the extracellular and intracellular life cycles, respectively. The sigL gene itself was induced by fivefolds prior to the invasion, and 5.3 folds during Caco-2 infection, further suggesting the role of SigL in intracellular growth.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Listeria monocytogenes
invasion
transcriptomics
RNA sequencing
Caco-2 cell
Megjelenés:Frontiers in Microbiology. - 13 (2022), p. 1-13. -
További szerzők:Boonmee, Atsadang Kijpornyongpan, Teeratas Tulsook, Kan Baranyi József (1956-) (biomatematikus) Chaturongakul, Soraya
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Intézményi repozitóriumban (DEA) tárolt változat
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