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001-es BibID:	BIBFORM106081
035-os BibID:	(cikkazonosító)15653 (WoS)000901106200001 (Scopus)85144543882 (PubMed)36555292
Első szerző:	Singlár Zoltán (biotechnológus)
Cím:	Genetic Manipulation of CB1 Cannabinoid Receptors Reveals a Role in Maintaining Proper Skeletal Muscle Morphology and Function in Mice / Singlár Zoltán, Ganbat Nyamkhuu, Szentesi Péter, Osgonsandag Nomin, Szabó László, Telek Andrea, Fodor János, Dienes Beatrix, Gönczi Mónika, Csernoch László, Sztretye Mónika
Dátum:	2022
ISSN:	1422-0067
Megjegyzések:	The endocannabinoid system (ECS) refers to a widespread signaling system and its alteration is implicated in a growing number of human diseases. Cannabinoid receptors (CBRs) are highly expressed in the central nervous system and many peripheral tissues. Evidence suggests that CB1Rs are expressed in human and murine skeletal muscle mainly in the cell membrane, but a subpopulation is present also in the mitochondria. However, very little is known about the latter population. To date, the connection between the function of CB1Rs and the regulation of intracellular Ca2+ signaling has not been investigated yet. Tamoxifen-inducible skeletal muscle-specific conditional CB1 knock-down (skmCB1-KD, hereafter referred to as Cre(+/-)) mice were used in this study for functional and morphological analysis. After confirming CB1R down-regulation on the mRNA and protein level, we performed in vitro muscle force measurements and found that peak twitch, tetanus, and fatigue were decreased significantly in Cre(+/-) mice. Resting intracellular calcium concentration, voltage dependence of the calcium transients as well as the activity dependent mitochondrial calcium uptake were essentially unaltered by Cnr1 gene manipulation. Nevertheless, we found striking differences in the ultrastructural architecture of the mitochondrial network of muscle tissue from the Cre(+/-) mice. Our results suggest a role of CB1Rs in maintaining physiological muscle function and morphology. Targeting ECS could be a potential tool in certain diseases, including muscular dystrophies where increased endocannabinoid levels have already been described.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk endocannabinoid system (ECS) cannabinoid receptor of type 1 (CBR1) skeletal muscle force contractility excitation-contraction coupling (ECC) mitochondria intracellular calcium
Megjelenés:	International Journal Of Molecular Sciences. - 23 : 24 (2022), p. 1-21. -
További szerzők:	Ganbat, Nyamkhuu Szentesi Péter (1967-) (élettanász) Osgonsandag, Nomin Szabó László (1994-) (molekuláris biológus) Telek Andrea (1977-) (élettanász) Fodor János (1973-) (élettanász, biotechnológus) Dienes Beatrix (1972-) (élettanász, molekuláris biológus) Gönczi Mónika (1974-) (élettanász) Csernoch László (1961-) (élettanász) Sztretye Mónika (1981-) (élettanász, elektrofiziológus)
Pályázati támogatás:	NKFIH-FK-142481 Egyéb NKFIH K-137600 Egyéb NKFIH 2019-2.1.11-TÉT-2019-00063 Egyéb ÚNKP-22-3-II-DE-239 Egyéb EFOP-3.6.3-VEKOP-16-2017-00009 EFOP TKP2021-EGA-18 Egyéb
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001-es BibID:	BIBFORM096247
035-os BibID:	(cikkazonosító)1415 (WOS)000699319500001 (Scopus)85114236612
Első szerző:	Singlár Zoltán (biotechnológus)
Cím:	Assessing the Potential of Nutraceuticals as Geroprotectors on Muscle Performance and Cognition in Aging Mice / Zoltán Singlár, Péter Szentesi, János Fodor, Ágnes Angyal, László Csernoch, Mónika Sztretye
Dátum:	2021
ISSN:	2076-3921
Megjegyzések:	Aging and frailty are associated with a decline in muscle force generation, which is a direct consequence of reduced muscle quantity and quality. Among the leading contributors to aging is the generation of reactive oxygen species, the byproducts of terminal oxidation. Their negative effects can be moderated via antioxidant supplementation. Krill oil and astaxanthin (AX) are nutraceuticals with a variety of health promoting, geroprotective, anti-inflammatory, anti-diabetic and anti-fatigue effects. In this work, we examined the functional effects of these two nutraceutical agents supplemented via pelleted chow in aging mice by examining in vivo and in vitro skeletal muscle function, along with aspects of intracellular and mitochondrial calcium homeostasis, as well as cognition and spatial memory. AX diet regimen limited weight gain compared to the control group; however, this phenomenon was not accompanied by muscle tissue mass decline. On the other hand, both AX and krill oil supplementation increased force production without altering calcium homeostasis during excitation-contraction coupling mechanism or mitochondrial calcium uptake processes. We also provide evidence of improved spatial memory and learning ability in aging mice because of krill oil supplementation. Taken together, our data favors the application of antioxidant nutraceuticals as geroprotectors to improve cognition and healthy aging by virtue of improved skeletal muscle force production.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk skeletal muscle calcium homeostasis muscle performance excitation-contraction coupling astaxanthin krill oil nutraceutical cognition mitochondrial calcium aging
Megjelenés:	Antioxidants. - 10 : 9 (2021), p. 1415. -
További szerzők:	Szentesi Péter (1967-) (élettanász) Fodor János (1973-) (élettanász, biotechnológus) Angyal Ágnes (1987-) (molekuláris biológus) Csernoch László (1961-) (élettanász) Sztretye Mónika (1981-) (élettanász, elektrofiziológus)
Pályázati támogatás:	TKP2020-IKA-04 Egyéb 2020-4.1.1-TKP2020 Egyéb PD-128370 Egyéb GINOP-2.3.4-15-2016-00002 GINOP EFOP-3.6.3-VEKOP-16-2017-00009 EFOP OpenA/DEENK/230/2021 Egyéb
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