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001-es BibID:BIBFORM106081
035-os BibID:(cikkazonosító)15653 (WoS)000901106200001 (Scopus)85144543882 (PubMed)36555292
Első szerző:Singlár Zoltán (biotechnológus)
Cím:Genetic Manipulation of CB1 Cannabinoid Receptors Reveals a Role in Maintaining Proper Skeletal Muscle Morphology and Function in Mice / Singlár Zoltán, Ganbat Nyamkhuu, Szentesi Péter, Osgonsandag Nomin, Szabó László, Telek Andrea, Fodor János, Dienes Beatrix, Gönczi Mónika, Csernoch László, Sztretye Mónika
Dátum:2022
ISSN:1422-0067
Megjegyzések:The endocannabinoid system (ECS) refers to a widespread signaling system and its alteration is implicated in a growing number of human diseases. Cannabinoid receptors (CBRs) are highly expressed in the central nervous system and many peripheral tissues. Evidence suggests that CB1Rs are expressed in human and murine skeletal muscle mainly in the cell membrane, but a subpopulation is present also in the mitochondria. However, very little is known about the latter population. To date, the connection between the function of CB1Rs and the regulation of intracellular Ca2+ signaling has not been investigated yet. Tamoxifen-inducible skeletal muscle-specific conditional CB1 knock-down (skmCB1-KD, hereafter referred to as Cre(+/-)) mice were used in this study for functional and morphological analysis. After confirming CB1R down-regulation on the mRNA and protein level, we performed in vitro muscle force measurements and found that peak twitch, tetanus, and fatigue were decreased significantly in Cre(+/-) mice. Resting intracellular calcium concentration, voltage dependence of the calcium transients as well as the activity dependent mitochondrial calcium uptake were essentially unaltered by Cnr1 gene manipulation. Nevertheless, we found striking differences in the ultrastructural architecture of the mitochondrial network of muscle tissue from the Cre(+/-) mice. Our results suggest a role of CB1Rs in maintaining physiological muscle function and morphology. Targeting ECS could be a potential tool in certain diseases, including muscular dystrophies where increased endocannabinoid levels have already been described.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
endocannabinoid system (ECS)
cannabinoid receptor of type 1 (CBR1)
skeletal muscle force
contractility
excitation-contraction coupling (ECC)
mitochondria
intracellular calcium
Megjelenés:International Journal Of Molecular Sciences. - 23 : 24 (2022), p. 1-21. -
További szerzők:Ganbat, Nyamkhuu Szentesi Péter (1967-) (élettanász) Osgonsandag, Nomin Szabó László (1994-) (molekuláris biológus) Telek Andrea (1977-) (élettanász) Fodor János (1973-) (élettanász, biotechnológus) Dienes Beatrix (1972-) (élettanász, molekuláris biológus) Gönczi Mónika (1974-) (élettanász) Csernoch László (1961-) (élettanász) Sztretye Mónika (1981-) (élettanász, elektrofiziológus)
Pályázati támogatás:NKFIH-FK-142481
Egyéb
NKFIH K-137600
Egyéb
NKFIH 2019-2.1.11-TÉT-2019-00063
Egyéb
ÚNKP-22-3-II-DE-239
Egyéb
EFOP-3.6.3-VEKOP-16-2017-00009
EFOP
TKP2021-EGA-18
Egyéb
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DOI
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2.

001-es BibID:BIBFORM083077
035-os BibID:(cikkazonosító)98 (WOS)000519877100052 (Scopus)85078738942 (PMID)31979219
Első szerző:Sztretye Mónika (élettanász, elektrofiziológus)
Cím:Improved Tetanic Force and Mitochondrial Calcium Homeostasis by Astaxanthin Treatment in Mouse Skeletal Muscle / Mónika Sztretye, Zoltán Singlár, László Szabó, Ágnes Angyal, Norbert Balogh, Faranak Vakilzadeh, Péter Szentesi, Beatrix Dienes, László Csernoch
Dátum:2020
ISSN:2076-3921
Megjegyzések:BACKGROUND: Astaxanthin (AX) a marine carotenoid is a powerful natural antioxidant which protects against oxidative stress and improves muscle performance. Retinol and its derivatives were described to affect lipid and energy metabolism. Up to date, the effects of AX and retinol on excitation-contraction coupling (ECC) in skeletal muscle are poorly described. METHODS: 18 C57Bl6 mice were divided into two groups: Control and AX supplemented in rodent chow for 4 weeks (AstaReal A1010). In vivo and in vitro force and intracellular calcium homeostasis was studied. In some experiments acute treatment with retinol was employed. RESULTS: The voltage activation of calcium transients (V50) were investigated in single flexor digitorum brevis isolated fibers under patch clamp and no significant changes were found following AX supplementation. Retinol shifted V50 towards more positive values and decreased the peak F/F0 of the calcium transients. The amplitude of tetani in the extensor digitorum longus was significantly higher in AX than in control group. Lastly, the mitochondrial calcium uptake was found to be less prominent in AX. CONCLUSION: AX supplementation increases in vitro tetanic force without affecting ECC and exerts a protecting effect on the mitochondria. Retinol treatment has an inhibitory effect on ECC in skeletal muscle.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
skeletal muscle
intracellular calcium
mitochondrial calcium
excitation contraction coupling
force
astaxanthin
retinol
Megjelenés:Antioxidants. - 9 : 2 (2020), p. 98. -
További szerzők:Singlár Zoltán (1994-) (biotechnológus) Szabó László (1994-) (molekuláris biológus) Angyal Ágnes (1987-) (molekuláris biológus) Balogh Norbert (1988-) (molekuláris biológus) Vakilzadeh, Faranak Szentesi Péter (1967-) (élettanász) Dienes Beatrix (1972-) (élettanász, molekuláris biológus) Csernoch László (1961-) (élettanász)
Pályázati támogatás:NKFIH-1150-6/2019
Egyéb
NKFIH PD-128370
Egyéb
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
DOI
Borító:
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