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001-es BibID:BIBFORM103405
035-os BibID:(cikkazonosító)2060 (WoS)000858038100001 (Scopus)85138606448
Első szerző:Beke-Varga Alexandra Edit (molekuláris biológus)
Cím:Suppressing the PI3K/AKT Pathway by miR-30d-5p Mimic Sensitizes Ovarian Cancer Cells to Cell Death Induced by High-Dose Estrogen / Varga Alexandra, Márton Éva, Markovics Arnold, Penyige András, Balogh István, Nagy Bálint, Szilágyi Melinda
Dátum:2022
ISSN:2227-9059
Megjegyzések:MicroRNAs are short non-coding RNA molecules that are involved in tumor development and are considered to be promising candidates in cancer therapy. Here, we studied the role of miR-30s in the pathophysiology of ovarian cancer. According to our results miR-30a-5p, miR-30d-5p, and miR-30e-5p were overexpressed in the estrogen receptor alpha (ER alpha)-expressing PEO1 cell line compared to A2780 that lacks this receptor. Furthermore, the expression of miR-30a-5p, miR-30d-5p, and miR-30e-5p were induced in response to high-dose estrogen treatment in PEO1 where intensive cell death was observed according to the induction of apoptosis and autophagy. Lacking or blocking ER alpha function reduced tolerance to high-dose estrogen that suggests the importance of ER alpha-mediated estrogen response in the maintenance of proliferation. MiR-30d-5p mimic reduced cell proliferation in both A2780 and PEO1. Furthermore, it decreased the tolerance of PEO1 cells to high-dose estrogen by blocking the ER alpha-mediated estrogen response. This was accompanied by decreased SOX4 expression that is thought to be involved in the regulation of the PI3K/AKT pathway. Blocking this pathway by AZD8835 led to the same results. MiR-30d-5p or AZD8835 sensitized PEO1 cells to tamoxifen. We suggest that miR-30d-5p might be a promising candidate in the therapy of ovarian cancer.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
miR-30
ovarian cancer
estrogen receptor
high-dose estrogen
cell proliferation
cell death
sox4
PI3K/AKT
cancer therapy
tamoxifen
Megjelenés:Biomedicines. - 10 : 9 (2022), p. 1-19. -
További szerzők:Márton Éva (1992-) (biológus) Markovics Arnold (1988-) (molekuláris biológus) Penyige András (1954-) (molekuláris genetikus) Balogh István (1972-) (molekuláris biológus, genetikus) Nagy Bálint (1956-) (molekuláris genetikus) Szilágyi Melinda (1984-) (biológus)
Pályázati támogatás:EFOP-3.6.3-VEKOP-16-2017-00009
EFOP
138021
OTKA
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
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2.

001-es BibID:BIBFORM108635
035-os BibID:(cikkazonosító)140 (Scopus)85148964475
Első szerző:Márton Éva (biológus)
Cím:Comparative Analysis of Transcriptomic Changes including mRNA and microRNA Expression Induced by the Xenoestrogens Zearalenone and Bisphenol A in Human Ovarian Cells / Éva Márton, Alexandra Varga, András Penyige, Zsuzsanna Birkó, István Balogh, Bálint Nagy, Melinda Szilágyi
Dátum:2023
ISSN:2072-6651
Megjegyzések:Xenoestrogens are natural or synthetic compounds that mimic the effect of endogenous estrogens and might cause cancer. We aimed to compare the global transcriptomic response to zearalenone (ZEA; mycotoxin) and bisphenol A (BPA; plastic additive) with the effect of physiological estradiol (E2) in the PEO1 human ovarian cell line by mRNA and microRNA sequencing. Estrogen exposure induced remarkable transcriptomic changes: 308, 288 and 63 genes were upregulated (log2FC > 1); 292, 260 and 45 genes were downregulated (log2FC < ?1) in response to E2 (10 nM), ZEA (10 nM) and BPA (100 nM), respectively. Furthermore, the expression of 13, 11 and 10 miRNAs changed significantly (log2FC > 1, or log2FC < ?1) after exposure to E2, ZEA and BPA, respectively. Functional enrichment analysis of the significantly differentially expressed genes and miRNAs revealed several pathways related to the regulation of cell proliferation and migration. The effect of E2 and ZEA was highly comparable: 407 genes were coregulated by these molecules. We could identify 83 genes that were regulated by all three treatments that might have a significant role in the estrogen response of ovarian cells. Furthermore, the downregulation of several miRNAs (miR-501- 5p, let-7a-2-3p, miR-26a-2-3p, miR-197-5p and miR-582-3p) was confirmed by qPCR, which might support the proliferative effect of estrogens in ovarian cells.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
xenoösztrogén
zearalenon
mikotoxin
biszfenol A
petefészekrák
transzkriptomika
mikroRNS
RNS szekvenálás
Megjelenés:Toxins. - 15 : 2 (2023), p. 1-22. -
További szerzők:Beke-Varga Alexandra Edit (1994-) (molekuláris biológus) Penyige András (1954-) (molekuláris genetikus) Hádáné Birkó Zsuzsanna (1971-) (molekuláris genetikus) Balogh István (1972-) (molekuláris biológus, genetikus) Nagy Bálint (1956-) (molekuláris genetikus) Szilágyi Melinda (1984-) (biológus)
Pályázati támogatás:FK138021
OTKA
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:
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