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001-es BibID:BIBFORM108313
035-os BibID:(WOS)000264270700005 (Scopus)59649102642
Első szerző:Diepeningen, Anne D. van
Cím:The het-c heterokaryon incompatibility gene in Aspergillus niger / Anne D. van Diepeningen, Károly Pál, Theo A. J. van der Lee, Rolf F. Hoekstra, Alfons J. M. Debets
Dátum:2009
ISSN:0953-7562
Megjegyzések:Heterokaryon incompatibility among Aspergillus niger strains is a widespread phenomenon that is observed as the inability to form stable heterokaryons. The genetic basis of heterokaryon incompatibility reactions is well established in some sexual filamentous fungi but largely unknown in presumed asexual species, such as A. niger. To test whether the genes that determine heterokaryon incompatibility in Neurospora crassa, such as het-c, vib-1 and pin-c, have a similar function in A. niger, we performed a short in silico search for homologues of these genes in the A. niger and several related genomes. For het-c, pin-c and vib- 1 we did indeed identify putative orthologues. We then screened a genetically diverse worldwide collection of incompatible black Aspergilli for polymorphisms in the het-c orthologue. No size variation was observed in the variable het-c indel region that determines the specificity in N. crassa. Sequence comparison showed only minor variation in the number of glutamine coding triplets. However, introduction of one of the three N. crassa alleles (hetc2) in A. niger by transformation resulted in an abortive phenotype, reminiscent of the heterokaryon incompatibility in N. crassa. We conclude that although the genes required are present and the het-c homologue could potentially function as a heterokaryon incompatibility gene, het-c has no direct function in heterokaryon incompatibility in A. niger because the necessary allelic variation is absent.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Mycological Research. - 113 : 2 (2009), p. 222-229. -
További szerzők:Pál Károly (1978-) (mikrobiológus) Lee, Theo A. J. van der Hoekstra, Rolf F. Debets, Alfons J. M.
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001-es BibID:BIBFORM117557
Első szerző:Pál Károly (mikrobiológus)
Cím:Sexual genes in the asexual filamentous fungus Aspergillus niger and related Aspergilli / Károly Pál, Anne D. van Diepeningen, János Varga, Alfons J. M. Debets, Rolf F. Hoekstra
Dátum:2008
Megjegyzések:Sexual reproduction is a general feature in higher eukaryotes, although the genetic machinery to produce sexually derived offspring can be very different. Contrary to the megabase-sized sexual chromosomes of animals, fungal genomes harbour much shorter sequences located at the MAT locus which governs the mating processes. For heterothallic fungi, the two mating-types are characterised by the presence of either of the two idiomorphs of the MAT locus, MAT-1 or MAT-2, while homothallic species may contain both MAT idiomorphs. The black mould Aspergillus niger is known as an asexual species, but some related presumed asexual species have recently been shown to have a cryptic sexual cycle. The scope of this work was to screen for and compare mating and meiosis related genes in the two sequenced A. niger genomes and to compare them to those in related Aspergilli. The MAT-1 mating type gene was found in both sequenced A. niger strains, a MAT-2 homologue was not detected in either, indicating that this species is either heterothallic or truly asexual. In comparison, the sequenced homothallic A. nidulans genome contains both MAT genes on different chromosomes, whereas the A. fumigatus, A. oryzae, and A. terreus genomes contain one MAT gene per sequenced genome. Many of the proteins known from Saccharomyces cerevisiae to be involved in meiosis proved well conserved in all five tested sexual and presumed asexual Aspergillus species. The Aspergilli also proved to have very similar sets of pheromone processing and response pathway proteins. Comparison of the two A. niger genomes for their gene sets show that many of these proteins may have essential pleiotropic functions next to mating and meiosis.
ISBN:978-90-8686-065-4
Tárgyszavak:Természettudományok Biológiai tudományok könyvfejezet
könyvrészlet
asexuality
mating type genes
meiosis related genes
Megjelenés:Aspergillus in the genomic era / ed. by János Varga, Robert A. Samson. - p. 107-131. -
További szerzők:Diepeningen, Anne D. van Varga János Debets, Alfons J. M. Hoekstra, Rolf F.
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3.

001-es BibID:BIBFORM108314
035-os BibID:(Scopus)38549136607 (WOS)000257097300004
Első szerző:Pál Károly (mikrobiológus)
Cím:Sexual and vegetative compatibility genes in the aspergilli / K. Pál, A. D. van Diepeningen, J. Varga, R. F. Hoekstra, P. S. Dyer, A. J. M. Debets
Dátum:2007
ISSN:0166-0616
Megjegyzések:Gene flow within populations can occur by sexual and/or parasexual means. Analyses of experimental and in silico work are presented relevant to possible gene flow within the aspergilli. First, the discovery of mating-type (MAT) genes within certain species of Aspergillus is described. The implications for self-fertility, sexuality in supposedly asexual species and possible uses as phylogenetic markers are discussed. Second, the results of data mining for heterokaryon incompatibility (het) and programmed cell death (PCD) related genes in the genomes of two heterokaryon incompatible isolates of the asexual species Aspergillus niger are reported. Het-genes regulate the formation of anastomoses and heterokaryons, may protect resources and prevent the spread of infectious genetic elements. Depending on the het locus involved, hetero-allelism is not tolerated and fusion of genetically different individuals leads to growth inhibition or cell death. The high natural level of heterokaryon incompatibility in A. niger blocks parasexual analysis of the het-genes involved, but in silico experiments in the sequenced genomes allow us to identify putative het-genes. Homologous sequences to known het- and PCD-genes were compared between different sexual and asexual species including different Aspergillus species, Sordariales and the yeast Saccharomyces cerevisiae. Both het- and PCD-genes were well conserved in A. niger. However some point mutations and other small differences between the het-genes in the two A. niger isolates examined may hint to functions in heterokaryon incompatibility reactions.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Studies In Mycology. - 59 (2007), p. 19-30. -
További szerzők:Diepeningen, Anne D. van Varga J. Hoekstra, Rolf F. Dyer, Paul S. Debets, Alfons J. M.
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Intézményi repozitóriumban (DEA) tárolt változat
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