CCL

Összesen 5 találat.
#/oldal:
Részletezés:
Rendezés:

1.

001-es BibID:BIBFORM118856
Első szerző:Szarvas József
Cím:Sequence analysis of partial tef1 and rpb2 genes of different Pleurotus eryngii isolates by means of PCR-RFLP / Szarvas József; Pál Károly; Geösel András
Dátum:2014
Megjegyzések:The Pleurotus eryngii species complex includes several varieties and certain groups with ambiguous taxonomic position. At the moment the following taxa are recognized: var. eryngii; var. ferulae /syn.: P. fuscus var. ferulae/; var. elaeoselini; var. nebrodensis; var. tingitanus; var. tuoliensis; P. hadamardii; P. fossulatus. In the course of our studies sequence analysis of the tef1á and rpb2 genes was performed, in order to distinguish varieties and reveal variability between isolates. Specific regions of the tef1á and rpb2 genes were amplified in PCR experiments. The fragments were cleaned, sequenced, aligned then BLAST searched against the NCBI GenBank nucleotide database. Some point mutations were detected in the sequences, which were used for selection of differentiating restriction enzymes for subsequent PCR-RFLP experiments. The tef1? gene sequences showed 100% identity in each strain. In contrast to that, point mutations were detected in the 21, 372 and 957 positions of the rpb2 sequences. In silico digestion was performed on the sequences and two restriction endonucleases, the BsmAI and TspDTI were selected for PCR-RFLP experiments. As a result of the digestions, the isolates could be grouped into two groups with both enzymes. At the same time, BLAST search with both amplified sequences did not give reliable information neither on varietas, nor on species level. The rpb2 locus, with its higher level of polymorphism, is a potential candidate for differentiation between varieties or identification on varietas level. Based on the results we plan to investigate which loci and molecular methods may be suitable for differentiation of isolates.
Tárgyszavak:Agrártudományok Élelmiszertudományok előadáskivonat
könyvrészlet
Pleurotus eryngii
tef1a
rpb2
PCR-RFLP
Megjelenés:Proceedings of 8th International Conference on Mushroom Biology and Mushroom Products Vol. 1. / ed. Manjit Singh. - p. 151-159
További szerzők:Pál Károly (1978-) (mikrobiológus) Geösel András
Internet cím:Szerző által megadott URL
Intézményi repozitóriumban (DEA) tárolt változat
Borító:

2.

001-es BibID:BIBFORM118852
Első szerző:Szarvas József
Cím:Comparative studies on the cultivation and phylogenetics of King Oyster Mushroom (Pleurotus eryngii (DC.: Fr.) Quél.) strains / Szarvas József; Pál Károly; Geösel András; Győrfi Júlia
Dátum:2011
Megjegyzések:The king oyster mushroom (Pleurotus eryngii) is still not a well known species amongst producers, despite its excellent taste and relatively easy cultivation technologies. Nevertheless, European growers show growing interest towards it. We collected king oyster mushroom strains from various habitats to get a better view about their vegetative growth, molecular based taxonomic relationship and cultivation parame- ters. In the in vitro experiments we investigated the growth of vegetative mycelia at various temperatures and pH. All strains were cultivated on lignocellulose substrate for 60 days under adequate climatic conditions and yield quantity, flushes, number and average weight of fruiting bodies were determined. Biological Efficiency (BE%) and Productivity (P%) were calculated for each strains. We investigated taxonomic relationships among P. eryngii isolates by means of RAPD-PCR method. Twenty-five random primers were tested and six of them supplied us with sufficient data for generation of a neighbour-joining tree. Results of the cultivation experiments showed that environmental conditions resulted in a very different growth rate of the various P. eryn- gii strains on different temperature and pH. Calculated for 100 kg sub- strate the highest yield was produced by the Ple-4V strain (41.5 kg), whereas the lowest yield was found at the PEL isolate (9 kg). The av- erage yield of the investigated strains was 27.53 kg. Average weight per fruiting body was 19.95 g. We found the highest BE% at the Ple-4V and the lowest BE% at the PEL strains, 156.18% and 28.52%, respectively. These results showed that cultivation properties of the isolates are very different. The neighbour-joining tree revealed the taxonomic relations amongst the Hungarian, Malaysian, Italian and Dutch isolates. No cor- relation was found between the taxonomical position and growth rate of the investigated isolates. The average yield showed that the P. eryn- gii might be a suitable alternative of the popular tree oyster mushroom (P. ostreatus). Our results can be useful for subsequent cross breeding experiments and the dataset might support further investigations.
Tárgyszavak:Agrártudományok Növénytermesztési és kertészeti tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
vegetative mycelium
RAPD-PCR
neighbour-joining tree
biological efficiency
Megjelenés:Acta Universitatis Sapientiae: Agriculture and Environment. - 3 (2011), p. 18-34. -
További szerzők:Pál Károly (1978-) (mikrobiológus) Geösel András Győrfi Júlia
Internet cím:Szerző által megadott URL
Intézményi repozitóriumban (DEA) tárolt változat
Borító:

3.

001-es BibID:BIBFORM118851
Első szerző:Szarvas József
Cím:Cultivation experiments of wild "King oyster mushroom" (Pleurotus eryngii) strains / Szarvas József; Geösel András; Kovács Violetta; Pál Károly; Győrfi Júlia
Dátum:2009
ISBN:978-963-503-397-3
Tárgyszavak:Agrártudományok Élelmiszertudományok előadáskivonat
könyvrészlet
king oyster mushroom
Pleurotus eryngii
mushroom production
Megjelenés:Lippay János - Ormos Imre - Vas Károly Tudományos Ülésszak : Összefoglalók : Élelmiszertudományi Kar. - p. 177. -
További szerzők:Geösel András Kovács Violetta Pál Károly (1978-) (mikrobiológus) Győrfi Júlia
Internet cím:Szerző által megadott URL
Intézményi repozitóriumban (DEA) tárolt változat
Borító:

4.

001-es BibID:BIBFORM118850
Első szerző:Szarvas József
Cím:Vad ördögszekér laskagomba (Pleurotus eryngii) törzsek termesztési vizsgálatai / Szarvas József; Geösel András; Kovács Violetta; Pál Károly; Győrfi Júlia
Dátum:2009
ISBN:978-963-503-397-3
Tárgyszavak:Agrártudományok Élelmiszertudományok előadáskivonat
könyvrészlet
ördögszekér laskagomba
Pleurotus eryngii
gombatermesztés
Megjelenés:Lippay János - Ormos Imre - Vas Károly Tudományos Ülésszak : Összefoglalók : Élelmiszertudományi Kar. - p. 176. -
További szerzők:Geösel András Kovács Violetta Pál Károly (1978-) (mikrobiológus) Győrfi Júlia
Internet cím:Szerző által megadott URL
Intézményi repozitóriumban (DEA) tárolt változat
Borító:

5.

001-es BibID:BIBFORM117333
035-os BibID:(WoS)000294637000020 (Scopus)84958184621
Első szerző:Szarvas József
Cím:Comparative studies of the cultivable king oyster mushroom [Pleurotus eryngii (DC.: Fr.) Quél.] isolates by RAPD-PCR method / J. Szarvas, A. Geösel, K. Pál, Z. Naár, J. Győrfi
Dátum:2011
ISSN:0139-3006 1588-2535
Megjegyzések:The king oyster mushroom (Pleurotus eryngii) is becoming more and more popular amongst the producers due to its excellent taste and relatively easy cultivation technology. Though investigations aiming to involve the mushroom in industrial cultivation had started in Hungary already in the 1950's, significant efforts were not made until 2002. In contrast to this, the volume of production in Europe and the United States has been growing continuously in the last decade. Although the species have been subjected to some taxonomical investigations, there are still a lot of contradictions in the taxonomic positioning of the P. eryngii species complex. In this study we investigated the genetic variability and taxonomic relationships among P. eryngii strains by using the RAPD-PCR method. Fifteen strains were analysed from our collection that represents mostly the Eastern-Hungarian habitats. Twenty-five random decamer primers were tested in the preliminary experiments and six were chosen that were used for binary coding. A neighbour-joining tree prepared from this matrix shows the coherence among the taxonomic relations and production sites of the potentially cultivable Hungarian strains.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény hazai lapban
folyóiratcikk
Pleurotus eryngii
species-complex
cultivation
RAPD-PCR
neighbour-joining tree
Megjelenés:Acta Alimentaria. - 40 : Suppl_1 (2011), p. 214-221. -
További szerzők:Geösel András Pál Károly (1978-) (mikrobiológus) Naár Zoltán Győrfi Júlia
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:
Rekordok letöltése1 
Corvina könyvtári katalógus v8.2.27 © 2023 Monguz kft. Minden jog fenntartva.